Variabilidade genética de Sugarcane mosaic virus, causando mosaico em milho no Brasil

Gonçalves, Marcos César; Galdeano, Diogo Manzano; Maia, Ivan de Godoy; Chagas, C. M.

doi:10.1590/s0100-204x2011000400004

Cited by 18 publications

(2 citation statements)

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“…4b , group 2). Cloning, sequencing, and restriction digestion analysis of SCMV infecting sugarcane in India [ 56 ] and maize in Brazil [ 57 ] showed that the N terminus of the coat protein is hypervariable. A similar analysis showed genetic diversity in SCMV coat protein sequence in Cameroon and Congo [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Metagenomic analysis of viruses associated with maize lethal necrosis in Kenya

Wamaitha

Nigam

Maina

et al. 2018

Virol J

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BackgroundMaize lethal necrosis is caused by a synergistic co-infection of Maize chlorotic mottle virus (MCMV) and a specific member of the Potyviridae, such as Sugarcane mosaic virus (SCMV), Wheat streak mosaic virus (WSMV) or Johnson grass mosaic virus (JGMV). Typical maize lethal necrosis symptoms include severe yellowing and leaf drying from the edges. In Kenya, we detected plants showing typical and atypical symptoms. Both groups of plants often tested negative for SCMV by ELISA.MethodsWe used next-generation sequencing to identify viruses associated to maize lethal necrosis in Kenya through a metagenomics analysis. Symptomatic and asymptomatic leaf samples were collected from maize and sorghum representing sixteen counties.ResultsComplete and partial genomes were assembled for MCMV, SCMV, Maize streak virus (MSV) and Maize yellow dwarf virus-RMV (MYDV-RMV). These four viruses (MCMV, SCMV, MSV and MYDV-RMV) were found together in 30 of 68 samples. A geographic analysis showed that these viruses are widely distributed in Kenya. Phylogenetic analyses of nucleotide sequences showed that MCMV, MYDV-RMV and MSV are similar to isolates from East Africa and other parts of the world. Single nucleotide polymorphism, nucleotide and polyprotein sequence alignments identified three genetically distinct groups of SCMV in Kenya. Variation mapped to sequences at the border of NIb and the coat protein. Partial genome sequences were obtained for other four potyviruses and one polerovirus.ConclusionOur results uncover the complexity of the maize lethal necrosis epidemic in Kenya. MCMV, SCMV, MSV and MYDV-RMV are widely distributed and infect both maize and sorghum. SCMV population in Kenya is diverse and consists of numerous strains that are genetically different to isolates from other parts of the world. Several potyviruses, and possibly poleroviruses, are also involved.Electronic supplementary materialThe online version of this article (10.1186/s12985-018-0999-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Metagenomic analysis of viruses associated with maize lethal necrosis in Kenya

Wamaitha

Nigam

Maina

et al. 2018

Virol J

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“…Sugarcane streak mosaic (SCSM) is a newly emerging disease in the country, which has been infecting that crop since 2017. The virus was known about fifteen years ago in Asian and Latino American sugarcane producing countries where it was first reported in 2004 and 2005 respectively in Brazil (Gonçalves et al, 2004;Gonçalves et al, 2007a;Gonçalves et al, 2007b;Gonçalves et al, 2011;Gonçalves et al, 2012) and Java, Indonesia (Chatenet et al, 2005;Kristini et al, 2006). It was reported that at least two strains of SCSMV were infecting sugarcane in Asia (Putra et al, 2015a).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Cane Genotypes Under Sprinkler Irrigation at Early Selection Stage for Tolerance to Sugarcane Streak Mosaic Virus (SCSMV) at Ferké Sugar Estates in Ivory Coast

Péné¹,

Béhou²

2020

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Sugarcane streak mosaic virus (SCSMV) became the major endemic disease of economic importance in Ivorian sugar estates almost two years ago, which spreads very fast across plantations and varieties. The study aimed to determine resistant sugarcane genotypes against SCSMV in Ferké sugar estates. It involved five experiments conducted at first selection stage under sprinkler irrigation, following a randomized complete block design (RCBD) with 20 to 30 different genotypes, two check varieties included, all in 4 replicates. Experiments were planted in October or December 2018, and expected to be harvested in November/December 2019 and 2020 as plant cane and first ratoon, respectively. Disease incidence and severity across all experiments were determined at 3 to 4 months, i.e. at early formative growth stage where symptoms due to SCSMV could be easily observed and recognized in the field. In each of the five selection trials conducted on both Ferké 1 & 2 sugar estates, highly significant differences in disease incidence and severity were observed between genotypes as well as crop cycles (plant cane and first ratoon). Except for one trial (B3-24 in Ferké 1), Genotype x crop cycle interactions were significant or highly significant, which showed that the majority of genotypes tested behaved differently from plant cane to first ratoon towards the disease. Particularly, the percentage of resistant genotypes decreased from 50 to 3.4% whereas that of highly susceptible ones increased from 4.2 to 92.4%. This shows the level of challenge to be tackled in the control of SCSM disease through sugarcane breeding and selection. At the end of the current selection stage under way, i.e. after harvest of first ratoon, only the best yielding genotypes among the resistant ones will undergo the advanced selection stage.

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Discovery of the first maize-infecting mastrevirus in the Americas using a vector-enabled metagenomics approach

et al. 2017

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The genus Mastrevirus (family Geminiviridae) is composed of single-stranded DNA viruses that infect mono- and dicotyledonous plants and are transmitted by leafhoppers. In South America, there have been only two previous reports of mastreviruses, both identified in sweet potatoes (from Peru and Uruguay). As part of a general viral surveillance program, we used a vector-enabled metagenomics (VEM) approach and sampled leafhoppers (Dalbulus maidis) in Itumbiara (State of Goiás), Brazil. High-throughput sequencing of viral DNA purified from the leafhopper sample revealed mastrevirus-like contigs. Using a set of abutting primers, a 2746-nt circular genome was recovered. The circular genome has a typical mastrevirus genome organization and shares <63% pairwise identity with other mastrevirus isolates from around the world. Therefore, the new mastrevirus was tentatively named "maize striate mosaic virus". Seventeen maize leaf samples were collected in the same field as the leafhoppers, and ten samples were found to be positive for this mastrevirus. Furthermore, the ten genomes recovered from the maize samples share >99% pairwise identity with the one from the leafhopper. This is the first report of a maize-infecting mastrevirus in the Americas, the first identified in a non-vegetatively propagated mastrevirus host in South America, and the first mastrevirus to be identified in Brazil.

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Variabilidade genética de Sugarcane mosaic virus, causando mosaico em milho no Brasil

Cited by 18 publications

References 19 publications

Metagenomic analysis of viruses associated with maize lethal necrosis in Kenya

Metagenomic analysis of viruses associated with maize lethal necrosis in Kenya

Evaluation of Cane Genotypes Under Sprinkler Irrigation at Early Selection Stage for Tolerance to Sugarcane Streak Mosaic Virus (SCSMV) at Ferké Sugar Estates in Ivory Coast

Discovery of the first maize-infecting mastrevirus in the Americas using a vector-enabled metagenomics approach

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