Diversidad genética de piracanjuba usada en programas de repoblación con marcadores microsatélites

Rodríguez-Rodríguez, Maria del Pilar; Lopera-Barrero, Nelson Maurício; Ribeiro, Ricardo Pereira; Povh, Jayme Aparecido; Vargas, Lauro; Sirol, Rodolfo Nardez; Jacometo, Carolina Bespalhok

doi:10.1590/s0100-204x2010000100008

Cited by 13 publications

(16 citation statements)

References 16 publications

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“…These markers have already been characterized and tested in natural mangaba tree populations from Brazilian Northeast [10] [13].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Accessions and First Generation Progeny of the Mangaba Genebank

Soares¹,

Clivati²,

Melo³

et al. 2018

AJPS

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Mangaba (Hancornia speciosa Gomes-Apocynaceae) is an important fruit tree in Brazil and has an unexplored stock germplasm collection. Commercial varieties are unavailable and little is known about its potential as a source of genetic diversity for molecular breeding programs. Progeny tests may improve studies on commercial variability and facilitate genetic improvement of the species. Our aim was to evaluate the genetic diversity and structure of accessions and progeny from the Mangaba Genebank of Embrapa Coastal Tablelands. We estimated the genetic diversity of 96 individuals using 4 SSR markers. The mean observed heterozygosity was lower than expected, showing an excess of homozygotes, corroborated by the positive coefficient of endogamy. The genetic diversity indexes (F st ) and Nei's unbiased genetic identity (GI) revealed, in general, moderate genetic diversity between accessions. The relationship between matrices and progenies showed that there may be a greater degree of genetic information sharing between the ABP1 and ABP2 accessions. The Bayesian analysis suggested the presence of two distinct groups of genotypes (K = 2). These results will assist future breeding programs and facilitate the conservation of mangaba.

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“…These markers have already been characterized and tested in natural mangaba tree populations from Brazilian Northeast [10] [13].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Accessions and First Generation Progeny of the Mangaba Genebank

Soares¹,

Clivati²,

Melo³

et al. 2018

AJPS

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“…Several studies have revealed that the formation of broodstocks with low genetic variability may cause difficulties related to endogamy cycles, adaptation capacity and survival of offspring (LOPERA-BARRERO et al, 2010a;RODRIGUEZ-RODRIGUEZ et al, 2010;LOPERA-BARRERO et al, 2014) and may affect the genetic potential of the species (MOREIRA et al, 2007). Low genetic diversity is negatively correlated with adaptation potential to changes in environmental conditions (RASHID et al, 2012;LYNCH et al, 1995;LOEW, 2000).…”

Section: Introductionmentioning

confidence: 99%

Genetic characteristics of Tambaqui broodstocks in the state of Rondônia, Brazil: implications on production and conservation

Ribeiro

Rodríguez-Rodríguez

Resende

et al. 2016

SCA

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In recent years, the genetic monitoring of broodstocks in fish farming has been greatly highlighted due to its importance in the management and improvement of their production and conservation. Current study evaluates the genetic diversity of four Tambaqui broodstocks (Colossoma macropomum) in the state of Rondônia (Brazil) and discusses activities towards the species´s correct production management and conservation. Nine primers were employed to analyze 94 specimens from four fish farms in the municipalities of Ji-Paraná (JP), Ouro Preto do Oeste (OP), Presidente Médici (PM) and Rolim de Moura (RM). Differences in the frequency of 38 fragments, with an exclusive fragment in JP and OP, were reported. High polymorphism (52.40 to 64.60%) and Shannon Index (0.313 to 0.382) rates were observed. The analysis of molecular variance (AMOVA) demonstrated that most variation is within each stock. The identity and genetic distance between the groups ranged between 0.927 and 0.954 and between 0.047 and 0.076 respectively, with shortest distance between the OPxPM and JPxRM groups. Genetic differentiation ranged from moderate to high (Fst = 0.081 to 0.179) and the number of migrants per generation was moderate (Nm = 3.83 to 6.24). As a rule, stocks showed high genetic variability and moderate / high differentiation and genetic distance between them. The results allowed direct conservation programs and increase the productivity of this species in fish farming through breeding programs. ResumoNos últimos anos, o monitoramento genético de estoques na piscicultura tem recebido grande destaque devido a sua importância no gerenciamento e melhora da sua produção e conservação. Dessa forma, o objetivo deste estudo foi avaliar de forma inédita a diversidade genética de quatro estoques de reprodutores Tambaqui (Colossoma macropomum) do estado de Rondônia (Brasil) e através dela, discutir ações que contribuam com o correto manejo produtivo e conservação dessa espécie. Utilizaram-se nove iniciadores para analisar 94 indivíduos de quatro pisciculturas dos municípios Ji-Paraná (JP), Ouro Preto do Oeste (OP), Presidente Medici (PM) e Rolim de Moura (RM). Foram encontradas diferenças nas frequências de 38 fragmentos, com um fragmento exclusivo em JP e OP. Altos valores de polimorfismo (52,40 a 64,60%) e índice de Shannon (0,313 a 0,382) foram observados. A análise de variância molecular (AMOVA) demostrou que a maior parte da variação está dentro de cada estoque. A identidade e distância genética entre os agrupamentos variou de 0,927 a 0,954 e 0,047 a 0,076, respectivamente, com menor distância entre os agrupamentos OPxPM e JPxRM. A diferenciação genética variou de moderada a alta (Fst = 0,081 a 0,179) e o número de migrantes por geração foi moderado (Nm = 3,83 a 6,24). De forma geral, os estoques apresentaram alta variabilidade genética e moderada/alta diferenciação e distancia genética entre eles. Os resultados permitirão direcionar programas de conservação e aumentar a produtividade dessa espécie na piscicultura através de program...

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“…Several strategies have been deployed to mitigate the environmental impact, among which may be mentioned fish restocking and increase in fish production. These events have made fish genetic studies highly necessary while molecular markers have proved to be useful for this end (JACOMENTO et al, 2010;LOPERA-BARRERO et al, 2015;POVH et al, 2011;RODRIGUEZ-RODRIGUEZ et al, 2010). There is a lack of information on the genetic assessment of stock enhancement programs for the tambaqui.…”

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“…Depending on the adopted reproduction management, the situation may occur in fish cultures mainly because of mating between kin fish, small N e and founding effect (LOPERA-BARRERO et al, 2015;RODRIGUEZ-RODRIGUEZ et al, 2010). Genetic divergence reached 0.1120 between the stocks, whereas genetic similarity featured 0,894.…”

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confidence: 99%

Genetic diversity of tambaqui broodstocks in stock enhancement programs

Neto

Ayres

Streit

et al. 2017

SCA

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Natural populations of tambaqui (Colossoma macropomum) have significantly decreased in recent decades especially due to human extraction activities. So that the environmental impact may be reduced, the restocking of fish and increase in fish production are enhanced. Genetic evaluations using molecular markers are essential for this purpose. Current study evaluates the genetic variability of two tambaqui broodstocks used in restocking programs. Sixty-five samples (33 samples from broodstock A and 32 samples from broodstock B) were collected. DNA was extracted from caudal fin samples, with the amplification of four microsatellite loci: Cm1A11 (EU685307) Cm1C8 (EU685308) Cm1F4 (EU685311) and Cm1H8 (EU685315). Fourteen alleles in the stock of broodstock A were produced, five alleles for Cm1A11 locus (230, 255, 260, 270 and 276 bp), three alleles Cm1C8 (239, 260, and 273 bp), two alleles Cm1F4 (211 and 245 bp), four alleles for Cm1H8 (275, 290, 320 and 331 bp) and two unique alleles were found for Cm1A11 loci (alleles 270 and 276 bp) and Cm1H8 (alleles 275 and 331 bp). In broodstock B, ten alleles were produced, the same alleles of the first stock except for alleles 270 and 276 bp in Cm1A11 locus and 275 and 331 bp in Cm1H8 locus. Broodstock A revealed low frequency alleles in Cm1A11 loci, Cm1C8, Cm1F4 and Cm1H8, whereas broodstock B had no locus with low allelic frequency. Loci Cm1A11, Cm1C8 and Cm1H8 exhibited significant deficit of heterozygotes in both broodstocks, revealing changes in Hardy-Weinberg equilibrium. Genetic diversity between stocks was 0.1120, whilst genetic similarity was 0.894, with F ST rate = 0.05, and Nm = 3.93, indicating gene flow between the two broodstocks. Results show that broodstocks are genetically closely related, with no great genetic variability. Strategies such as a previous genetic analysis of breeding with its marking, use of a large Ne crossing between the most genetically divergent specimens, and the introduction of new genetic material to broodstocks may maximize genetic diversity and minimize inbreeding within the next generation.

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Diversidad genética de piracanjuba usada en programas de repoblación con marcadores microsatélites

Cited by 13 publications

References 16 publications

Genetic Diversity of Accessions and First Generation Progeny of the Mangaba Genebank

Genetic Diversity of Accessions and First Generation Progeny of the Mangaba Genebank

Genetic characteristics of Tambaqui broodstocks in the state of Rondônia, Brazil: implications on production and conservation

Genetic diversity of tambaqui broodstocks in stock enhancement programs

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