Transformação genética de laranja 'Valência' com o gene cecropin MB39

Paoli, Luis Gustavo de; Boscariol-Camargo, Raquel L.; Harakava, Ricardo; Mendes, Beatriz Madalena Januzzi; Filho, Francisco de Assis Alves Mourão

doi:10.1590/s0100-204x2007001100020

Cited by 12 publications

(7 citation statements)

References 9 publications

(10 reference statements)

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“…We have applied many of these strategies in an attempt to develop citrus with improved disease resistance, including genes to enhance SAR , and special studies on preferentially expressed gene promoters (Paoli et al 2007). Furthermore, in genetic engineering of citrus for resistance to Xcc, we and other researchers produced transgenic citrus expressing R-genes such as Xa21 from Oryza sativa (Omar et al 2007;Boscariol 2004;Mendes et al 2009) and the attacin A antimicrobial peptide gene (Boscariol et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Transgenic Sweet Orange (Citrus sinensis L. Osbeck) Expressing the attacin A Gene for Resistance to Xanthomonas citri subsp. citri

et al. 2009

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Genetic transformation with genes that code for antimicrobial peptides has been an important strategy used to control bacterial diseases in fruit crops, including apples, pears, and citrus. Asian citrus canker (ACC) caused by Xanthomonas citri subsp. citri Schaad et al. (Xcc) is a very destructive disease, which affects the citrus industry in most citrus-producing areas of the world. Here, we report the production of genetically transformed Natal, Pera, and Valencia sweet orange cultivars (Citrus sinensis L. Osbeck) with the insect-derived attacin A (attA) gene and the evaluation of the transgenic plants for resistance to Xcc. Agrobacterium tumefaciens Smith and Towns-mediated genetic transformation experiments involving these cultivars led to the regeneration of 23 different lines. Genetically transformed plants were identified by polymerase chain reaction, and transgene integration was confirmed by Southern blot analyses. Transcription of attA gene was detected by Northern blot analysis in all plants, except for one Natal sweet orange transformation event. Transgenic lines were multiplied by grafting onto Rangpur lime rootstock plants (Citrus limonia Osbeck) and sprayinoculated with an Xcc suspension (10 6 cfu mL −1 ). Experiments were repeated three times in a completely randomized design with seven to ten replicates. Disease severity was determined in all transgenic lines and in the control (nontransgenic) plants 30 days after inoculation. Four transgenic lines of Valencia sweet orange showed a significant reduction in disease severity caused by Xcc. These reductions ranged from 58.3% to 77.8%, corresponding to only 0.16-0.30% of leaf diseased area as opposed to 0.72% on control plants. One transgenic line of Natal sweet orange was significantly more resistant to Xcc, with a reduction of 45.2% comparing to the control plants, with only 0.14% of leaf diseased area. Genetically transformed Pera sweet orange plants expressing attA gene did not show a significant enhanced resistance to Xcc, probably due to its genetic background, which is naturally more resistant to this pathogen. The potential effect of attacin A antimicrobial peptide to control ACC may be related to the genetic background of each sweet orange cultivar regarding their natural resistance to the pathogen.

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Section: Discussionmentioning

confidence: 99%

Transgenic Sweet Orange (Citrus sinensis L. Osbeck) Expressing the attacin A Gene for Resistance to Xanthomonas citri subsp. citri

et al. 2009

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“…Research is ongoing to develop genetically modified varieties with resistance to X. fastidiosa (De Paoli et al, 2007), The effectiveness of resistant or tolerant varieties is high, but, considering the very wide host range of X. fastidiosa and the time needed to breed and introduce new resistant varieties, the feasibility is rated as low.…”

Section: Resistant or Less Susceptible Varietiesmentioning

confidence: 99%

Statement of EFSA on host plants, entry and spread pathways and risk reduction options for Xylella fastidiosa Wells et al.

2013

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Following a request from the European Commission, EFSA was asked to provide urgent scientific and technical assistance on the plant pathogenic bacterium Xylella fastidiosa. X. fastidiosa was detected in olive trees in Lecce province in Apulia, Italy, in October 2013. This is the first outbreak of X. fastidiosa under field conditions in the European Union. EFSA reviewed the host range and vectors, the pathways for entry and spread and the risk reduction options. Known hosts include many cultivated and spontaneous plants common in Europe, however a range of European wild plant species would meet this bacterium for the first time, increasing uncertainty on the host range. All xylem-fluid feeding insects in Europe should be regarded as potential vectors of X. fastidiosa and identification of the vector in the Apulian outbreak is pending. The main entry pathway for X. fastidiosa is the movement of plants for planting. Infective vectors of X. fastidiosa transported on plant consignments are also of concern. The only route for natural spread of X. fastidiosa is by insect vectors that generally fly short distances up to 100 metres, but can be transported by wind over long distance. The movement of infected plants for planting is the most efficient way for long-distance dispersal of X. fastidiosa. There is no record of successful eradication of X. fastidiosa once established outdoors due to the broad host range of the pathogen and of its vectors. Strategies for prevention of introduction from areas where the pathogen is present and for containment of outbreak should focus on the two main pathways and be based on integrated system approach combining, when applicable, the most effective options.

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“…(AZEVEDO, 2005). Recentemente, Paoli et al (2007) obtiveram plantas transgênicas de laranja doce com o gene da cecropina (cecropin MB39), dirigido pelo promotor do gene da fenilalanina amônia-liase que confere expressão gênica nos vasos do xilema, visando resistência a X. fastidiosa. Paoli (2007) avaliou plantas de laranja 'Valência' transformadas com o gene da cecropina (cecropin MB39) quanto à resistência a X. fastidiosa e uma planta revelou resistência ao patógeno.…”

Section: Transformação Genética Em Citros Para Resistência a Doençasunclassified

Avaliação da resistência a Xylella fastidiosa Wells et al. e Xanthomonas axonopodis pv. citri Vauterin et al. em plantas transgênicas de Citrus sinensis L. Osbeck expressando os genes atacina A ou Xa21

Cardoso¹

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Avaliação da resistência a Xylella fastidiosa Wells et al. e Xanthomonas axonopodis pv. citri Vauterin et al. em plantas transgênicas de Citrus sinensis L. Osbeck expressando os genes atacina A ou Xa21 Suane Coutinho Cardoso Tese apresentada para obtenção do título de Doutor em Agronomia. Área de concentração: Fitotecnia Piracicaba 2008 Suane Coutinho Cardoso Engenheiro Agrônomo Avaliação da resistência a Xylella fastidiosa Wells et al. e Xanthomonas axonopodis pv. citri Vauterin et al. em plantas transgênicas de Citrus sinensis L. Osbeck expressando os genes atacina A ou Xa21 Orientador: Prof. Dr. FRANCISCO DE ASSIS ALVES MOURÃO FILHO Tese apresentada para obtenção do título de Doutor em Agronomia. Área de concentração: Fitotecnia Piracicaba 2008 Dados Internacionais de Catalogação na Publicação (CIP) DIVISÃO DE BIBLIOTECA E DOCUMENTAÇÃO-ESALQ/USP Cardoso, Suane Coutinho Avaliação da resistência a Xylella fastidiosa Wells et al. e Xanthomonas axonopodis pv. citri Vauterin et al. em plantas transgênicas de Citrus sinensis L. Osbeck expressando os genes atacina A ou Xa21 / Suane Coutinho Cardoso.-Piracicaba, 2008. 99 p. : il.

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Transformação genética de laranja 'Valência' com o gene cecropin MB39

Cited by 12 publications

References 9 publications

Transgenic Sweet Orange (Citrus sinensis L. Osbeck) Expressing the attacin A Gene for Resistance to Xanthomonas citri subsp. citri

Transgenic Sweet Orange (Citrus sinensis L. Osbeck) Expressing the attacin A Gene for Resistance to Xanthomonas citri subsp. citri

Statement of EFSA on host plants, entry and spread pathways and risk reduction options for Xylella fastidiosa Wells et al.

Avaliação da resistência a Xylella fastidiosa Wells et al. e Xanthomonas axonopodis pv. citri Vauterin et al. em plantas transgênicas de Citrus sinensis L. Osbeck expressando os genes atacina A ou Xa21

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