Diferenciação molecular de cultivares elites de bananeira

Jesus, O. N. de; Câmara, Terezinha Rangel; Ferreira, Cláudia Fortes; Silva, Sebastião de Oliveira e; Pestana, Kátia Nogueira; Soares, Taliane Leila

doi:10.1590/s0100-204x2006001200008

Cited by 7 publications

(6 citation statements)

References 20 publications

(17 reference statements)

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“…Esse diplóide é amplamente utilizado em vários programas de melhoramento de banana como fonte de alelos para resistência à sigatoka-negra, causada por Mycosphaerella fi jiensis (Pillay et al, 2001 (Creste et al, 2003(Creste et al, , 2004Jesus et al, 2006). Atualmente, o programa de melhoramento genético de banana da Embrapa Mandioca e Fruticultura Tropical conta com 99 diplóides melhorados, com resistência genética às principais pragas, incluindo as sigatokas-amarela e negra e o mal-do-panamá, além de características agronômicas desejáveis, tais como: porte reduzido, alta produtividade, tolerância à seca etc.…”

Section: Resultsunclassified

Variabilidade genética estimada entre diplóides de banana por meio de marcadores microssatélites

Amorim¹,

Reis

Santos-Serejo³

et al. 2008

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi estimar a divergência genética entre 38 diplóides de banana do programa de melhoramento da Embrapa Mandioca e Fruticultura Tropical, incluindo genótipos melhorados, cultivados e selvagens, por meio de 15 marcadores microssatélites ou SSR. As similaridades genéticas, com base no coefi ciente de Jaccard, foram utilizadas para fazer o agrupamento dos genótipos pelo método UPGMA. O número de alelos obtidos foi 113, com média de 7,53 alelos por iniciador. A similaridade genética média foi de 0,22, e variou de 0,028 a 0,48, o que indica existência de variabilidade genética entre os genótipos. A análise de grupos, com base no polimorfi smo de microssatélites, não pôde separar completamente os híbridos melhorados, cultivados e selvagens. Alguns diplóides agruparam-se com base em sua origem geográfi ca, entre eles Musa ornata e IAC-1, e Tjau Lagada e Lidi, enquanto que, em outros, nenhuma relação foi estabelecida. Houve tendência de agrupamento entre os diplóides aparentados, como: SH3263 e 8694-20, 4279-01 e 9179-03, 1304-06 e 5854-03, 86B79-10 e 7341-03, 86B79-12 e 0337-02, e 9194-04 e 4154-08.Termos para indexação: Musa, diplóides, divergência genética, híbridos, marcadores moleculares. Genetic variability estimated in banana diploids through microsatellite markersAbstract -The objective of this work was to estimate the genetic diversity between 38 banana diploids from the banana breeding program of Embrapa Mandioca e Fruticultura Tropical (Embrapa Cassava and Tropical Fruits), including improved hybrids, cultivated and wild species, using fi fteen SSR markers. Genetic similarities were utilized to cluster genotypes through UPGMA, based on Jaccard coeffi cient. The average number of alleles per primer was 7.53, with a total of 113 alleles identifi ed. The average similarity was 0.22, and ranged from 0.028 to 0.48, indicating the existence of genetic variability between genotypes. The clustering analysis based on microsatellite polymorphism could not completely separate improved hybrids, cultivated and wild species. Some diploids grouped according to their geographic origins, among which Musa ornata and IAC-1, and Tjau Lagada and Lidi, while in others no relation was established. There was a trend of clustering among related diploids as: SH3263 and 8694-20; 4279-01 and 9179-03; 1304-06 and 5854-03; 86B79-10 and 7341-03; 86B79-12 and 0337-02; and 9194-04 and 4154-08.

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Section: Resultsunclassified

Variabilidade genética estimada entre diplóides de banana por meio de marcadores microssatélites

Amorim¹,

Reis

Santos-Serejo³

et al. 2008

Pesq. agropec. bras.

Self Cite

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“…Bhat and Jarret (1995) demonstrated the application of RAPD markers on the characterization of germplasm of Musa spp., and it has been possible to distinguish clones that were morphologically identical. Jesus et al (2006) working with the techniques of microsatellite and RAPD obtained similar responses with the two techniques, for the separation of genotypes according to the genomic group and origin of hybrids. This shows that the technique of RAPD has considerable reliability, since it repeats results generated by other techniques.…”

Section: Introductionmentioning

confidence: 78%

Contrastant banana accessions for resistance to the burrowing nematode, based on molecular markers RAPD

et al. 2009

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This work aimed to proceed molecular characterization of seven banana accessions (Borneo, Grand Naine, 1304-06, 4249-05, 0337-02, 0323-03 and 4279-06) resistance to the nematode Radopholus similis. These accessions were selected taking in account the reproduction factor (RF) among 26 banana genotypes from a working collection belonging to Embrapa Mandioca e Fruticultura Tropical.The genomic DNA of the seven accessions was extracted, and 36 decamere primers had been used to obtain RAPD markers. The resulting markers were converted into a matrix of binary data. From that matrix the genetic distance between the accessions were estimated, for further clustering and graphic dispersion analyses. From a total of 521 RAPD markers generated, 420 (81%) were polymorphic, including 140 (27%) potentially promising for application on works related to genetic mapping of the resistance to R. similis. OPE-15, OPH-17, and OPG-09 were the primers that contributed to the highest number of bands promising for genetic mapping of resistance (12, 8, and 8, respectively). The genetic distances between accessions ranged from 0.106 to 0.455, with the longest one observed between cv. Borneo and the genotype 4279-06, considered as highly susceptible and resistant, respectively, to the nematode according to the RF. The graphic dispersion distinguished three groups of accessions, and most of resistant genotypes clustered together in the same group. The most contrastant genotypes for resistance (Borneo and 4249-05) were separated by a genetic distance of 0.374, and possessed a total of 114 polymorphic bands promising for genetic mapping of resistance. In addition, the results of pathogenicity tests were congruent with those obtained by RAPD analyses.

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“…Studies have been performed in blueberries (Silva et al, 2008), Barbados cherries (Salla et al, 2002), avocados (Sharon et al, 1997;Borrone et al, 2009), and bananas (Jesus et al, 2006(Jesus et al, , 2009Amorim et al, 2009) with molecular markers. The use of these markers requires only small amounts of plant DNA, ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 11410-11428 (2015) and they are easily detected by PCR while having wide genomic coverage (Buso et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of microsatellite loci from libraries derived from the wild diploid 'Calcutta 4' and 'Ouro' banana cultivars

et al. 2015

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Diferenciação molecular de cultivares elites de bananeira

Cited by 7 publications

References 20 publications

Variabilidade genética estimada entre diplóides de banana por meio de marcadores microssatélites

Variabilidade genética estimada entre diplóides de banana por meio de marcadores microssatélites

Contrastant banana accessions for resistance to the burrowing nematode, based on molecular markers RAPD

Evaluation of microsatellite loci from libraries derived from the wild diploid 'Calcutta 4' and 'Ouro' banana cultivars

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