Lipoxygenase activities during development of root and nodule of soybean

Abstract

-The objective of this work was to evaluate root and nodule soybean lipoxygenases in Doko cultivar and in a near isogenic line lacking seed lipoxygenases, inoculated and uninoculated with Bradyrhizobium elkanii. The lipoxygenase activities from roots collected at 3, 5, 9, 13, 18 and 28 days post-inoculation and from nodules collected at 13, 18 and 28 days post-inoculation were measured. The pH-activity profiles from root and nodules suggested that the lipoxygenases pool expressed in these organs from Doko cultivar and triple-null near isogenic lines are similar. The root lipoxygenase activity of Doko and triple-null lines, inoculated and uninoculated, reduced over time. The highest lipoxygenase activity observed at the beginning of root formation suggests the involvement of this enzyme in growth and development of this organ. However, for nodules an expressive increase of lipoxygenase activity was noticed 28 days post-inoculation. Root and nodule showed, at least, two mobility groups for lipoxygenases in immunoblottings, with approximately 94 and 97 kDa.Index terms: Glycine max, triple-null lines, nodulation. Atividade de lipoxigenases durante o desenvolvimento da raiz e do nódulo de plantas de sojaResumo -O objetivo deste trabalho foi avaliar lipoxigenases de raízes e nódulos de plantas de soja da variedade Doko e da linhagem triplo nula derivada, desprovida das lipoxigenases da semente, com e sem inoculação de Bradyrhizobium elkanii. A atividade de lipoxigenase foi avaliada em raízes coletadas aos 3, 5, 9, 13, 18 e 28 dias após a inoculação e em nódulos coletados aos 13, 18 e 28 dias após a inoculação. Os perfis de pH-atividade de raiz e nódulo sugerem que o "pool" de lipoxigenases expresso nesses órgãos não difere nos dois genótipos. A atividade de lipoxigenases nas raízes de Doko e Doko triplo nula, com e sem inoculação, declinou com o passar do tempo. A maior atividade de lipoxigenases no início de formação da raiz sugere o envolvimento desta enzima no crescimento e desenvolvimento deste órgão. Nos nódulos houve um aumento acentuado na atividade de lipoxigenase aos 28 dias após a inoculação. Dois grupos de mobilidade com aproximadamente 94 e 97 kDa foram encontrados nos "immunoblottings" para lipoxigenases de raiz e de nódulo.Termos para indexação: Glycine max, linhagem triplo nula, nodulação.

Quantitative Proteomics Reveals That Peroxidases Play Key Roles in Post-flooding Recovery in Soybean Roots

“…At the mRNA level, lipoxygenase was up-regulated under flooding but was steadily down-regulated during recovery. The slight increase in lipoxygenase at the protein level during recovery was considerably less than pre-flooding levels, confirming the role of this enzyme in growth and development of roots during germination, as reported earlier . The observed differences between protein abundance and mRNA expression suggest that post-translational modification affects the function of lipoxygenase.…”

“…The slight increase in lipoxygenase at the protein level during recovery was considerably less than pre-flooding levels, confirming the role of this enzyme in growth and development of roots during germination, as reported earlier. 44 The observed differences between protein abundance and mRNA expression suggest that post-translational modification affects the function of lipoxygenase. The slight increase in lipoxygenase suggests plant's attempt to resume growth suppressed by flooding.…”

Rice Overexpressing OsNUC1-S Reveals Differential Gene Expression Leading to Yield Loss Reduction after Salt Stress at the Booting Stage

“…Interestingly, the molecular function enrichment was found for only linoleate 13S-lipoxygenase activity. This enzyme is involved in plant growth and development [15], and also in the wounding response through jasmonic acid (JA) signaling [16,17] (Figure 7A).…”

Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development

“…The only observed difference is related to the presence of a band, of 95kDa molecular weight, in cultivar 'BRS267'. As studies dealing with electrophoresis of soybean grains proteins identified a lipoxygenase with molecular weight of 95kDa (JUNGHANS et al, 2004), it is possible that the band here verified corresponds to this protein. The catalytic action exerted by lipoxygenase on polyunsaturated fatty acids, linoleic and linolenic acid, of the soybean grains, is a major factor responsible for the appearance of carbonyl compounds, which promote the flavor of the grains (MARTINS et al, 2002).…”