Stomatal analysis of citrus somatic hybrids obtained by protoplast fusion

Costa, Mirco; Almeida, Weliton Antônio Bastos de; Filho, Francisco de Assis Alves Mourão; Mendes, Beatriz Madalena Januzzi; Rodriguez, Adriana Pinheiro Martinelli

doi:10.1590/s0100-204x2004000300015

Cited by 6 publications

(7 citation statements)

References 5 publications

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“…Scanning electron microscope analysis has been previously used as an effective method to confirm protoplast fusant. Costa et al (2004) applied the SEM analysis to evaluate leaf epidermis morphological characteristics of three citrus somatic hybrids, compared to their parents. Although morphological feature can be signs for primary identification of the fusants, the DNA based genetic markers could provide the detailed information of the nuclear constitution and genetic relationship between the fusant and its parents.…”

Section: Discussionmentioning

confidence: 99%

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Identification of protoplast fusion strain Fhhh by randomly amplified polymorphic DNA

Zhao

Zhang

et al. 2009

World J Microbiol Biotechnol

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A functional strain Fhhh was constructed through protoplast fusion of three parental strains (Phanerochaete chrysosporium, Saccharomyces cerevisiae and native bacterium YZ) to improve the degradation efficiency of purified terephthalic acid wastewater. Randomly amplified polymorphic DNA (RAPD) and scanning electron microscope (SEM) analysis were applied to identify and confirm the fusant Fhhh through phenotypic and genetic relationship. The result of SEM analysis demonstrated that the cell shape of fusant Fhhh differed from all three parental strains. RAPD analysis of 40 arbitrary primers generated a total of 1,135 bands. The genetic similarity indices between Fhhh and parental strains Phanerochaete chrysosporium (PC), Saccharomyces cerevisiae (SC) and native bacterium (YZ) were 34.01%, 33.16%, and 35.97%, respectively. The targeted-gene PCR results showed that Fhhh inherited the DNA fragments of mnp and lip genes from parental strain PC and FLO1 gene fragment from parental strain SC. Our results suggested protoplast fusion technique may be considered as a promising technique in environmental pollution control.

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Section: Discussionmentioning

confidence: 99%

“…Costa et al (2004) applied the technology to evaluate leaf epidermis morphological characteristics of three citrus somatic hybrids, compared to their parents. Random amplification polymorphic DNA (RAPD), which scans numerous loci in the genome is a useful genetic marker method (Welsh and McClelland 1990;Williams et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Identification of protoplast fusion strain Fhhh by randomly amplified polymorphic DNA

Zhao

Zhang

et al. 2009

World J Microbiol Biotechnol

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“…Examinando estatisticamente os dados (Tabela 2), utilizando análise de variância (ANOVA) a três fatores (cultivar, tempo e concentração), conclui-se que existe diferença significativa entre as cultivares (p = 0,039 (GANTAIT et al, 2011). A técnica mais precisa na análise de estômatos utiliza microscópio eletrônico de varredura (COSTA et al, 2004). Entretanto, a disponibilidade do equipamento limita o seu uso em determinados grupos de pesquisa.…”

Section: Resultsunclassified

Indução de poliploidia em mandioca

Silva¹

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The induction of polyploidy in cassava The polyploidy played an important role in the improvement of many species of plants, especially in cereals including intergeneric hybrids. The process of polyploidy usually produces new and desirable phenotypes or provides support for crosses of plants in breeding programs. This research work reports the result of polyploidy induction in two cassava cultivars ('Porquinho' and 'Vassourinha') by different colchicine concentrations (0.05 %, 0.10 % and 0.15 %) in culture medium exposed for 48 or 96 hours. Regenerated plants were examined cytologically by flow cytometry and chromosome counting. Additionally, analysis of the size and frequency of stomata were performed and the results showed that can be used for screening of the treated plants. Treatment with 0.1% colchicine in the culture medium for 96 hours showed a higher number of tetraploid plants produced about the number of regenerated plants in both cultivars evaluated. No significant differences among colchicine doses and duration times treatment were found in the number of tetraploids found in each treatment. However, we observed a significant difference in tetraploid production between the cultivars analyzed, indicating that there may be a genetic response to induction of chromosome doubling with colchicine.

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“…A densidade estomática e tamanho de células-guarda de estômatos, de folhas de híbridos somáticos tetraplóides de citros e das espécies parentais foram avaliados por Costa et al (2004).…”

Section: Identificação E Caracterização De Plantas Poliplóides De Citrosunclassified

Obtenção de plantas autotetraplóides de variedades de porta-enxertos de citros

Júnior¹,

da²

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Stomatal analysis of citrus somatic hybrids obtained by protoplast fusion

Cited by 6 publications

References 5 publications

Identification of protoplast fusion strain Fhhh by randomly amplified polymorphic DNA

Identification of protoplast fusion strain Fhhh by randomly amplified polymorphic DNA

Indução de poliploidia em mandioca

Obtenção de plantas autotetraplóides de variedades de porta-enxertos de citros

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