2003
DOI: 10.1590/s0100-204x2003000700015
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Abstract: -Even though much improvement has been made in plant transformation methods, the screening of transgenic plants is often a laborious work. Most approaches for detecting the transgene in transformed plants are still time-consuming, and can be quite expensive. The objective of this study was to search for a simpler method to screen for transgenic plants. The infiltration of kanamycin (100 mg/mL) into tobacco leaves resulted in conspicuous chlorotic spots on the non-transgenic plant leaves, while no spots were se… Show more

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Cited by 2 publications
(2 citation statements)
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“…Even though much improvement has been made, however, this is often a laborious work. Most approaches for detecting the transgene in transformed plants are time consuming and can be quite expensive (Freitas et al, 2003). Screening transformed plants for gene integration and expression is a process that involves different techniques, including DNA-and RNA-blot hybridization PCR and ELISA analysis (de Kochko et al, 1994).…”
Section: Transgenic Plants and Its Detectionmentioning
confidence: 99%
“…Even though much improvement has been made, however, this is often a laborious work. Most approaches for detecting the transgene in transformed plants are time consuming and can be quite expensive (Freitas et al, 2003). Screening transformed plants for gene integration and expression is a process that involves different techniques, including DNA-and RNA-blot hybridization PCR and ELISA analysis (de Kochko et al, 1994).…”
Section: Transgenic Plants and Its Detectionmentioning
confidence: 99%
“…As plantas que demonstraram total resistência à canamicina foram transferidas para vasos e cultivadas até a produção de frutos e obtenção de T2. (FREITAS-ASTUA et al, 2003; XIANG, HAN; OLIVER, 1999; WEIDE; KOORNNEEF; ZABEL, 1989). As análises foram executadas em termociclador de acordo com a seguinte programação: para as plantas transformadas com o gene CDR-1: 1 ciclo de 10 segundos a 98°C, seguido de 30 ciclos de 1 segundo a 98°C, 5 segundos a 58°C, 15 segundos a 72°C e por fim, 1 ciclo de 1 minuto a 72°C.…”
Section: Seleção Das Linhagens T1 E T2 Das Plantas Transgênicas Por Cunclassified