Absorção de macronutrientes por explantes de bananeira in vitro

Diniz, Josefa Diva Nogueira; Gonçalves, Antônio Natal; Hernández, Fernando Felipe Ferreyra

doi:10.1590/s0100-204x1999000700012

Cited by 12 publications

(9 citation statements)

References 12 publications

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“…Diniz et al (1999) pointed out that low levels of phosphorous in in vitro culture media may be responsible for the low growth rates observed in some species. This author considered the interaction of phosphorous with other medium salts (the latter often in excess in the medium) to be responsible for the small amounts of phosphorous present in plants maintained in this type of environment.…”

Section: Resultsmentioning

confidence: 99%

Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

et al. 2010

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Annona glabra is a tropical species that has significant agronomic potential in terms of furnishing fruits for in natura consumption and for the production of phyto-pharmaceuticals. In vitro cultivation has been considered the most promising form of propagation for this species, although large scale utilization of this technique is currently limited by high rates of leaf abscission, reduced rates of explant multiplication and slow bud growth. The present work evaluated the effects of different cytokinins on mineral accumulation in shoots of A. glabra cultivated in vitro, and their effects on growth and survival of these plants. Buds of A. glabra were cultivated in Wood Plant Medium (WPM) in the presence of 6-benzilaminopurine (BAP), thidiazuron (TDZ), kinetin (KIN), and zeatin (ZEA). KIN and BAP use resulted in the greatest growth, largest accumulation of dry mass and leaf area development, as well as the greatest survival rate during in vitro cultivation of this species. All cytokinins tested stimulated large accumulations of nitrogen and boron in shoots, but diminished levels of calcium as compared to controls.Index terms: Growth regulators, Annonaceae, mineral nutrition. RESUMOAnnona glabra é uma espécie frutífera tropical que apresenta elevado potencial agronômico pelo fornecimento de frutos para o consumo in natura e pela produção de fitofármacos. O cultivo in vitro tem sido preconizado como a forma mais adequada de propagação para essa espécie, embora sua utilização em larga escala ainda seja limitada pela elevada taxa de abscisão foliar, reduzida taxa de multiplicação dos explantes e crescimento lento das brotações. Objetivou-se, neste trabalho, avaliar o efeito de citocininas sobre o acúmulo de minerais nas brotações de A. glabra cultivadas in vitro e seus reflexos sobre o crescimento e sobrevivência das plantas nesse tipo de ambiente. Brotações de A. glabra foram cultivadas em meio Wood Plant Medium (WPM), na presença de 6-benzilaminopurina (BAP), thidiazuron (TDZ), cinetina (KIN) e zeatina (ZEA). A utilização de KIN e BAP induziu maior crescimento, maior acúmulo de massa seca, maior desenvolvimento da área foliar e maior taxa de sobrevivência das plantas durante o cultivo in vitro dessa espécie. Todas as fontes de citocininas testadas estimularam maiores acúmulos de nitrogênio e boro nas brotações e menores de cálcio.Termos para indexação: Reguladores de crescimento, Annonaceae, nutrição mineral.

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Section: Resultsmentioning

confidence: 99%

Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

et al. 2010

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“…Ciência Rural, v.40, n.2, fev, 2010. livres de doenças, também possibilita produção de um número elevado de mudas em curto espaço de tempo (SOUZA et al, 1999). Um dos fatores que ainda prejudica a utilização de mudas micropropagadas é o alto custo para produção, e muitos trabalhos apresentam alternativas, visando a otimizar o processo de multiplicação in vitro de mudas de bananeira (DINIZ et al, 1999;KODYM & ZAPATA-ARIAS 1999;KODYM & ZAPATA-ARIAS, 2001;BERNARDI et al, 2004;MADHULATHA et al, 2004;ERIG & SCHUCH, 2005).…”

Section: Introductionunclassified

Composição do meio de cultura e condições ambientais para germinação de grãos de pólen de porta-enxertos de pereira

Chagas¹,

Pio²,

Chagas³

et al. 2010

Cienc. Rural

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Volume do frasco e consistência do meio de cultura na multiplicação in vitro da bananeira 'Maçã'.Volume do frasco e consistência do meio de cultura na multiplicação in vitro da bananeira 'Maçã' Flask size and growth medium consistence in the in vitro multiplication of 'Maçã' banana tree RESUMO A propagação in vitro da bananeira possibilita produzir, em larga escala, mudas livres de doenças em curto espaço de tempo. Para otimizar o processo de micropropagação, pode-se modificar a metodologia de produção das mudas, levando-se em conta também a manutenção da qualidade. O objetivo deste trabalho foi avaliar a influência do volume do frasco e da consistência do meio de cultura na taxa de multiplicação e no desenvolvimento de mudas de bananeira 'Maçã', propagadas in vitro. A partir de ápices caulinares, foram realizados quatro subcultivos (S2, S3, S4 e S5) em meio MS modificado com metade da concentração dos macronutrientes, acrescido de 30g L -1 de sacarose e 2,5mg L -1 de BAP (6 benzilaminopurina), em tratamento fatorial 2x2: dois tipos de meio de cultivo (semissólido por adição de 1,6g L -1 de Phytagel™ e líquido estacionário) e frascos de dois volumes (250 e 500mL). As plântulas foram induzidas ao enraizamento in vitro e aclimatizadas em estufas, em bandejas de 72 células, e posteriormente cultivadas em sacos de polipropileno de 17x18cm. O cultivo meio líquido em frasco de 500mL pode ser adotado com sucesso para multiplicação de mudas de banana 'Maçã', tendo sido observada maior taxa de multiplicação (25,27 brotos/frasco), melhor adaptação no início da aclimatização e desempenho similar aos demais tratamentos após 60 dias de cultivo em sacos de polipropileno.Palavras-chave: micropropagação, Musa spp., cultura de tecidos, meio líquido. ABSTRACTThe in vitro propagation of the banana is accomplished to produce large quantities of seedlings free from diseases. Micropropagation process can be optimized changing the method, but also considering plantlet quality. The objective of this work was to evaluate the influence of the flask size and of the growth medium consistence on the multiplication rate and on the initial plantlet development of in vitro propagated 'Maçã' banana tree. Starting from stem apexes, four subcultures were accomplished (S2, S3, S4 and S5) in MS medium modified with the half of the concentration of the macronutrients, added of 30g L -1 of sucrose, 2.5mg L -1 of BAP (6-benzylaminopurine) in factorial design 2x2: two types of growth media (semi solid by addition of 1.6g L -1 Phytagel™ and stationary liquid), and two flask sizes (250 and 500mL). Plantlets were induced in vitro rooting and acclimatization in greenhouse using trays with 72 cells and later grown in 17x18cm polypropylene bags. Cultivation in liquid medium bottle of 500mL can be successfully adopted for propagation of 'Maçã' banana plantlets because it had larger multiplication rate (25.27), better adaptation in the initial stage of acclimatization and similar performance to the other treatments after 60 days of cultivation in the bags.

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“…Freitas et al (2015), when working with 'Cleopatra' mandarin plantlets, reported that 1.0 mg L -1 brassinosteroids associated with arbuscular mycorrhizal fungi led to a 15.4% increase in the aerial dry mass nitrogen content. Diniz et al (1999) evaluated in vitro macronutrient absorption by banana explants, and reported that nitrogen content was higher during the first 10 days, decreasing as a function of culture time. According to the authors, this decrease in nitrogen content in tissues may occur as a function of the dilution effect, due to higher dry mass production.…”

Section: Variation Causes F Valuesmentioning

confidence: 99%

Humic acids and brassinosteroid application effects on pineapple plantlet growth and nutrition during the aclimatization phase

Santos¹,

Carvalho²,

Silva³

et al. 2018

Afr. J. Agric. Res.

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Humic acid and brassinosteroid applications may be an alternative to decrease the pineapple plantlet acclimatization in in vitro cultivation, since promising results have been observed when these substances were independently applied in other propagation methods. In this sense, the aim of the present study was to evaluate the effects of humic acids and brassinosteroid application on 'BRS Vitória' pineapple plantlets grown from in vitro cultivation during acclimatization. A randomized block design was used in a 5x2x4 factorial scheme, at five brassinosteroid doses (0, 0.25, 0.50, 0.75, 1.0 mg L-1) in the presence and absence of humic acids during four sampling periods (60, 90, 120 and 150 days after transplanting), with five replicates for each treatment. BIOBRÁS-16 was used as the brassinosteroid source, and the organic soil conditioner Agrolmin HF ® was used as the humic acid source. Plantlets were collected for evaluation every 30 days from 60 days after transplanting. The number of plantlet leaves, length and root mass were higher in the humic acid treatment without brassinosteroid application. Leaf, nitrogen, phosphorus, potassium, calcium and magnesium contents were of 13.04, 1.77, 40.2, 8.79 and 3.17 mg kg-1 , respectively. Nitrogen and potassium contents in the plantlets decreased, while phosphorus contents increased as a function of acclimatization time, regardless of treatment.

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Absorção de macronutrientes por explantes de bananeira in vitro

Cited by 12 publications

References 12 publications

Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

Composição do meio de cultura e condições ambientais para germinação de grãos de pólen de porta-enxertos de pereira

Humic acids and brassinosteroid application effects on pineapple plantlet growth and nutrition during the aclimatization phase

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