Polycystic echinococcosis in the state of Acre, Brazil: contribution to patient diagnosis, treatment and prognosis

Siqueira, Nilton Ghiotti de; Siqueira, Cláudia Maria Villar Maziero de; Rodrigues-Silva, Rosângela; Soares, Manoel do Carmo Pereira; Póvoa, Marinete Marins

doi:10.1590/s0074-02762013000500001

Cited by 10 publications

(13 citation statements)

References 17 publications

(26 reference statements)

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“…To the best of our knowledge, this article contains the first report of molecularly detected E. vogeli infection, reinforcing the role of domestic dogs as sources of infection for humans in the municipality of Sena Madureira, where human polycystic echinococcosis has been previously reported [20, 45]. Given that E. granulosus ( s.s .)…”

Section: Discussionmentioning

confidence: 53%

“…In northern Brazil, polycystic echinococcosis has been reported in bush dogs [14, 18], pacas [43, 44] and humans [20, 45]. Although dogs play the double function of companion and hunting (mainly pacas) animals in these communities and are often fed raw paca viscera [14], the presence of E. vogeli has not yet been investigated.…”

Section: Discussionmentioning

confidence: 99%

“…The E. vogeli sylvatic cycle is based on a specific predator–prey relationship between the bush dog ( Speothos venaticus ), an indigenous canid in South America, and pacas [14, 18]. Within tropical forests, domestic dogs often accompany their owners during hunting [19] and are fed raw viscera as a reward for their activities [20]. Despite strong indications this practice can give rise to the synantropic cycle [14], it remains unclear whether such canids become infected with E. vogeli in the North Region of Brazil.…”

Section: Introductionmentioning

confidence: 99%

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First molecular identification of Echinococcus vogeli and Echinococcus granulosus (sensu stricto) G1 revealed in feces of domestic dogs (Canis familiaris) from Acre, Brazil

et al. 2017

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Background Echinococcus granulosus (sensu lato) (s.l.) and Echinococcus vogeli are causative agents of chronic zoonotic diseases such as cystic and polycystic echinococcosis, respectively. In Brazil, polycystic echinococcosis has a restricted geographical distribution in the North Region, while cystic echinococcosis is observed in the South Region. Domestic dogs (Canis familiaris) fed with raw viscera represent a risk factor for E. granulosus (s.l.) infection in the South Region. Although this practice is frequent, it remains unclear whether domestic dogs are infected with E. vogeli in the state of Acre, located in the Amazon basin in the North Region of Brazil. The aim of this study was to investigate this gap in the polycystic echinococcosis epidemiology.MethodsSixty-five fecal samples were collected from the ground in five municipalities (Sena Madureira, n = 14; Rio Branco, n = 06; Bujari, n = 06; Xapuri, n = 30; and Epitaciolândia, n = 09) located in the state of Acre, northern Brazil. The samples were screened for parasites by copro-PCR using the cox1 gene associated with automated sequencing.Results Echinococcus vogeli was molecularly confirmed in a sample from Sena Madureira and E. granulosus (sensu stricto) (s.s.) (G1) in a sample from Rio Branco.ConclusionsThese findings indicate that molecular assays are useful in typing Echinococcus taxa from fecal samples of dogs in northern Brazil. The present study is the first molecular record of E. vogeli in domestic dogs found in the state of Acre, reinforcing their role as a source of infection for humans. Because E. granulosus (s.s.) (G1) was detected for the first time in the North Region, from the epidemiological standpoint this finding is highly relevant, because it expands the known geographical distribution, which was previously restricted to the South Region of Brazil.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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First molecular identification of Echinococcus vogeli and Echinococcus granulosus (sensu stricto) G1 revealed in feces of domestic dogs (Canis familiaris) from Acre, Brazil

et al. 2017

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“…The complexity of echinococcosis and the heterogeneity of clinical cases require a thorough understanding of the parasite-host relationship and the parasite phenotype at each developmental stage, to choose the best available diagnostic tool acceptable for clinical practice. Early diagnosis of echinococcosis may decrease or even prevent the disease from worsening and minimize the risks to the patient (Siqueira et al, 2013). To date, several approaches have been described for laboratory diagnosis and follow-up of CE and alveolar echinococcosis (AE) infections, including detection of antibodies, antigens and cytokines (Siles-Lucas et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…All criteria had to be met to include the patient in the study. All samples were kindly supplied by Dr Nilton Guiotti Siqueira from the Federal University of Acre, and followed the inclusion criteria established in previous studies (D 'Alessandro, 1997;Siqueira et al, 2013).…”

Section: Serum Samplesmentioning

confidence: 99%

Evaluation of two heterologous recombinant antigens for the serological diagnosis of human polycystic echinococcosis

et al. 2022

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Polycystic echinococcosis (PE) is a zoonosis endemic in the Neotropical region of the Americas. It is caused by the larval stage of the cestode Echinococcus vogeli, which develops as harmful cysts that slowly grow in the liver, lungs and other organs of humans and other host species. Human PE diagnosis is usually based on clinical and epidemiological aspects and imaging techniques, often requiring confirmation by immunological assays. The currently available serological tests for detecting antibodies against Echinococcus spp. are mostly based on complex, variable and poorly characterized mixtures of native parasite antigens, which impairs specificity and/or sensitivity. In this scenario, the evaluation of well-characterized alternative antigens is urgently needed for the improvement of PE diagnosis. Here, two subunits (AgB8/1 and AgB8/2) of the major secretory antigen from Echinococcus granulosus (antigen B (AgB)), of diagnostic value for cystic echinococcosis, were validated for PE diagnosis. These antigens, produced as pure recombinant proteins (rAgB8/1 and rAgB8/2) in Escherichia coli, allowed detecting specific immunoglobulin G antibodies in sera from PE patients in an enzyme-linked immunosorbent assay, with sensitivities of 83.72% and 81.40%, respectively, and specificities of 83.12% and 80.09%, respectively. The use of recombinant proteins overcomes difficulties to obtain parasite material and reduced non-specific reactions and costs. Our results demonstrated reproducibility and accuracy high enough to be considered valid according to the acceptance criteria for Food and Drug Administration assay validation. This qualifies rAgB8/1 and rAgB8/2 as potential substitutes for the currently used parasite crude or partially purified antigens.

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Polycystic Echinococcosis (Polycystic Neotropical Echinococcus)

Coyle¹

2020

Hunter's Tropical Medicine and Emerging Infectious Diseases

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Polycystic echinococcosis in the state of Acre, Brazil: contribution to patient diagnosis, treatment and prognosis

Cited by 10 publications

References 17 publications

First molecular identification of Echinococcus vogeli and Echinococcus granulosus (sensu stricto) G1 revealed in feces of domestic dogs (Canis familiaris) from Acre, Brazil

First molecular identification of Echinococcus vogeli and Echinococcus granulosus (sensu stricto) G1 revealed in feces of domestic dogs (Canis familiaris) from Acre, Brazil

Evaluation of two heterologous recombinant antigens for the serological diagnosis of human polycystic echinococcosis

Polycystic Echinococcosis (Polycystic Neotropical Echinococcus)

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