The relationship between genetic variability and the susceptibility of Biomphalaria alexandrina snails to Schistosoma mansoni infection

Mohamed, Azza H.; El-Din, Ahmed T. Sharaf; Mohamed, A. M.; Habib, Mohamed R.

doi:10.1590/s0074-02762012000300006

Cited by 16 publications

(8 citation statements)

References 44 publications

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“…This prevalence rate is considerably higher than expected and has many indications. For example, it may indicate a recent human-water contact in spots with abundant occurrence of infected Biomphalaria alexandrina (Ehrenberg, 1831), the main intermediate host for S. mansoni in Egypt (lotFy et al 2005, MoHaMed et al 2012, abou el-naGa 2013. Thus, efforts should be directed to identifying such spots and to take appropriate measures.…”

Section: Introductionmentioning

confidence: 99%

Control of infection of Biomphalaria alexandrina (Ehrenberg, 1831) with Schistosoma mansoni Sambon, 1907 using Eucalyptus camaldulensis

Mossalem¹,

Habib²,

Ghareeb³

2018

Folia Malacol.

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Schistosomiasis infection can be interrupted if the development of miracidia to cercariae in Biomphalaria alexandrina (Ehrenberg) is blocked. This requires an efficient snail's antioxidant system which can be complemented with an exogenous antioxidant source to alleviate the oxidative stress of infection. For this purpose, leaves of Eucalyptus camaldulensis were air-dried, extracted with aqueous methanol (85%) and defatted with petroleum ether. The obtained defatted extract was used to prepare extracts with different solvents. Among these, ethyl acetate showed the highest antioxidant activity and was chosen for the experiments. The effect of ethyl acetate extract on the infection, survivorship, as well as levels of malondialdehyde (MDA), catalase (CAT) and reduced glutathione (GSH) in B. alexandrina were measured in control, untreated infected and treated infected snails on 1, 10, and 30 days post infection (dpi). The snails exposed to ethyl acetate extract showed a significant reduction in the infection rate compared to those infected and untreated. Moreover, the extract decreased the level of MDA and increased CAT and GSH activities in the haemolymph and tissues of the treated snails. The results suggest that ethyl acetate extract from leaves of E. camaldulensis can be used as an antiparasitic compound against the intramolluscan phase of S. mansoni Sambon, 1907.

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Section: Introductionmentioning

confidence: 99%

Control of infection of Biomphalaria alexandrina (Ehrenberg, 1831) with Schistosoma mansoni Sambon, 1907 using Eucalyptus camaldulensis

Mossalem¹,

Habib²,

Ghareeb³

2018

Folia Malacol.

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“…Biomphalaria which serves as an obligatory host for the larval stage, by which humans get infected. Among many identified species of Biomphalaria, Biomphalaria alexandrina is the intermediate host of S. mansoni in Egypt (Morgan et al, 2001;Lotfy, DeJong, Black, & Loker, 2005;Abou-El-Naga, El-Nassery, Allam, Shaat, & Mady, 2011;Mohamed, El-Din, Mohamed, & Habib, 2012).…”

mentioning

confidence: 99%

Total protein composition of young and adult Biomphalaria alexandrina snails with different compatibilities to Schistosoma mansoni infection

Sadaka

Abou-El-Naga

Amer

et al. 2016

RBT

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In the current study differentiation of the total proteins among Biomphalaria alexandrina snails at different ages was done to identify the proteins responsible for the snails’ compatibility outcome. The work was conducted on snails that differ in their age and the genetic backgrounds. Four subgroups from the progeny of self reproduced susceptible and resistant snails were studies. Infection rates of these subgroups (young susceptible, adult susceptible, young resistant and adult resistant) were 90%, 75%, 40% and 0% respectively. Using Sodium dodecyl sulphate polyacrylamide gel electrophoresis, differences in protein expression were shown between adult and young snails in different subgroups. Dice similarity coefficient was calculated to determine percentage band sharing among the experimental subgroups. The results revealed that the combination of similarities between both age and compatibility status of the snails has led to the highest similarity coefficient followed by the combination of similarities of both genetic origin and age although they differ in the compatibility status. On the other hand, the differences in the genetic background, age and compatibility status have led to the least similarity index. It was also found that in young snails the genetic background plays the main role in determination of their compatibility, while the internal defense system has the upper hand in determination of adult compatibility. Further characterization of the shared protein bands among the studied subgroups is needed to clarify their role in host-parasite relationship.

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“…These primers might amplify very restricted areas of the genomes of B. glabrata and B. tenagophila specific for resistance or susceptibility, which are not present in B. alexandrina snails. Interestingly, one of these primers, the OPA-01 primer, resulted in high genetic variability between B. alexandrina strains from different provinces in Egypt using the RAPD-PCR technique [37]. The present study showed that with five out of the eight utilized primers, specific bands of different lengths were amplified in the resistant and susceptible phenotype snails.…”

Section: Discussionmentioning

confidence: 73%

Genetic Variation betweenBiomphalaria alexandrinaSnails Susceptible and Resistant toSchistosoma mansoniInfection

el-Nassery

Abou-El-Naga

Allam

et al. 2013

BioMed Research International

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Much effort has been made to control schistosomiasis infection in Egypt. However, enduring effects from such strategies have not yet been achieved. In this study, we sought to determine the genetic variability related to the interaction between Biomphalaria alexandrina snails and Schistosoma mansoni. Using RAPD-PCR with eight (10 mers) random primers, we were able to determine the polymorphic markers that differed between snails susceptible and resistant to Schistosoma mansoni infection using five primers out of the eight. Our results suggest that the RAPD-PCR technique is an efficient means by which to compare genomes and to detect genetic variations between schistosomiasis intermediate hosts. The RAPD technique with the above-noted primers can identify genomic markers that are specifically related to the Biomphalaria alexandrina/Schistosoma mansoni relationship in the absence of specific nucleotide sequence information. This approach could be used in epidemiologic surveys to investigate genetic diversity among Biomphalaria alexandrina snails. The ability to determine resistant markers in Biomphalaria alexandrina snails could potentially lead to further studies that use refractory snails as agents to control the spread of schistosomiasis.

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The relationship between genetic variability and the susceptibility of Biomphalaria alexandrina snails to Schistosoma mansoni infection

Abstract: In the present study, Biomphalaria snails collected from five Egyptian governorates (Giza, Fayoum, Kafr ElSheikh, Ismailia and Damietta)

Cited by 16 publications

References 44 publications

Control of infection of Biomphalaria alexandrina (Ehrenberg, 1831) with Schistosoma mansoni Sambon, 1907 using Eucalyptus camaldulensis

Control of infection of Biomphalaria alexandrina (Ehrenberg, 1831) with Schistosoma mansoni Sambon, 1907 using Eucalyptus camaldulensis

Total protein composition of young and adult Biomphalaria alexandrina snails with different compatibilities to Schistosoma mansoni infection

Genetic Variation betweenBiomphalaria alexandrinaSnails Susceptible and Resistant toSchistosoma mansoniInfection

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