Field optimisation of MosquiTRAP sampling for monitoring Aedes aegypti Linnaeus (Diptera: Culicidae)

Resende, Marcelo Carvalho de; Ázara, Tatiana Mingote Ferreira de; Costa, Ione Oliveira; Heringer, Laila Costa; Andrade, Mateus Rezende; Acebal, José Luiz; Eiras, Álvaro Eduardo

doi:10.1590/s0074-02762012000300002

Cited by 20 publications

(15 citation statements)

References 24 publications

(38 reference statements)

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“…Nevertheless, there is little evidence to suggest that larval surveys are more accurate at estimating adult population sizes or transmission risk. Few studies have investigated the MosquiTRAP in this regard; however, available studies suggest that adult capture is associated with transmission risk ( Focks 2003 , de Resende et al 2012 ).…”

Section: Discussionmentioning

confidence: 99%

A comparison of larval, ovitrap and MosquiTRAP surveillance for Aedes (Stegomyia) aegypti

Resende

Silva

Ellis

et al. 2013

Mem. Inst. Oswaldo Cruz

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In Brazil, the entomological surveillance of Aedes (Stegomyia) aegypti is performed by government-mandated larval surveys. In this study, the sensitivities of an adult sticky trap and traditional surveillance methodologies were compared. The study was performed over a 12-week period in a residential neighbourhood of the municipality of Pedro Leopoldo, state of Minas Gerais, Brazil. An ovitrap and a MosquiTRAP were placed at opposite ends of each neighbourhood block (60 traps in total) and inspections were performed weekly. The study revealed significant correlations of moderate strength between the larval survey, ovitrap and MosquiTRAP measurements. A positive relationship was observed between temperature, adult capture measurements and egg collections, whereas precipitation and frequency of rainy days exhibited a negative relationship.

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Section: Discussionmentioning

confidence: 99%

A comparison of larval, ovitrap and MosquiTRAP surveillance for Aedes (Stegomyia) aegypti

Resende

Silva

Ellis

et al. 2013

Mem. Inst. Oswaldo Cruz

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“…We tested a commercially available synthetic mosquito odor lure tablet (AtrAedes), which contains odors identified from volatile grass infusions ( Panicum maximum , Jacq.) and has been used in other oviposition traps designed to lure gravid Aedes aegypti mosquitoes [5,13,14]. Teabags, organic water (from a local ditch) and oak leaf ( Quercus spp.)…”

Section: Methodsmentioning

confidence: 99%

Development and evaluation of a novel contamination device that targets multiple life-stages of Aedes aegypti

et al. 2014

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BackgroundThe increasing global threat of Dengue demands new and easily applicable vector control methods. Ovitraps provide a low-tech and inexpensive means to combat Dengue vectors. Here we describe the development and optimization process of a novel contamination device that targets multiple life-stages of the Aedes aegypti mosquito. Special focus is directed to the diverse array of control agents deployed in this trap, covering adulticidal, larvicidal and autodissemination impacts.MethodsDifferent trap prototypes and their parts are described, including a floater to contaminate alighting gravid mosquitoes. The attractiveness of the trap, different odor lures and floater design were studied using fluorescent powder adhering to mosquito legs and via choice tests. We demonstrate the mosquitocidal impacts of the control agents: a combination of the larvicide pyriproxyfen and the adulticidal fungus Beauveria bassiana. The impact of pyriproxyfen was determined in free-flight dissemination experiments. The effect on larval development inside the trap and in surrounding breeding sites was measured, as well as survival impacts on recaptured adults.ResultsThe developmental process resulted in a design that consists of a black 3 Liter water-filled container with a ring-shaped floater supporting vertically placed gauze dusted with the control agents. On average, 90% of the mosquitoes in the fluorescence experiments made contact with the gauze on the floater. Studies on attractants indicated that a yeast-containing tablet was the most attractive odor lure. Furthermore, the fungus Beauveria bassiana was able to significantly increase mortality of the free-flying adults compared to controls. Dissemination of pyriproxyfen led to >90% larval mortality in alternative breeding sites and 100% larval mortality in the trap itself, against a control mortality of around 5%.ConclusionThis ovitrap is a promising new tool in the battle against Dengue. It has proven to be attractive to Aedes aegypti mosquitoes and effective in contaminating these with Beauveria bassiana. Furthermore, we show that the larvicide pyriproxyfen is successfully disseminated to breeding sites close to the trap. Its low production and operating costs enable large scale deployment in Dengue-affected locations.

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“…The disease brings considerable health, social, and economic problems to endemic areas. With the ongoing search for an efficient vaccine and an antiviral drug, prevention is still the best way to control the disease, which is possible through vector control in several forms (Bäck and Lundkvist , Resende et al , Resende et al ). Various trap models to capture and measure the density of the vector have been designed, and their use in many countries has shown them to be a better strategy than classical surveillance methods for monitoring (Regis et al , Regis et al ).…”

Section: Introductionmentioning

confidence: 99%

Dengue virus detection in Aedes aegypti larvae from southeastern Brazil

Cecílio¹,

Júnior²,

Tótola

et al. 2015

Journal of Vector Ecology

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The transmission of dengue, the most important arthropod-borne viral disease in Brazil, has been intensified over the past decades, along with the accompanying expansion and adaptation of its Aedes vectors. In the present study, we mapped dengue vectors in Ouro Preto and Ouro Branco, Minas Gerais, by installing ovitraps in 32 public schools. The traps were examined monthly between September, 2011 through July, 2012 and November, 2012 to April, 2013. The larvae were reared until the fourth stadium and identified according to species. The presence of dengue virus was detected by real time PCR and agarose gel electrophoresis. A total of 1,945 eggs was collected during the 17 months of the study. The Ovitrap Positivity Index (OPI) ranged from 0 to 28.13% and the Eggs Density Index (EDI) ranged from 0 to 59.9. The predominant species was Aedes aegypti, with 84.9% of the hatched larvae. Although the collection was low when compared to other ovitraps studies, vertical transmission could be detected. Of the 54 pools, dengue virus was detected in four Ae. aegypti pools. Journal of Vector Ecology 40 (1): 71-74. 2015.

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Field optimisation of MosquiTRAP sampling for monitoring Aedes aegypti Linnaeus (Diptera: Culicidae)

Cited by 20 publications

References 24 publications

A comparison of larval, ovitrap and MosquiTRAP surveillance for Aedes (Stegomyia) aegypti

A comparison of larval, ovitrap and MosquiTRAP surveillance for Aedes (Stegomyia) aegypti

Development and evaluation of a novel contamination device that targets multiple life-stages of Aedes aegypti

Dengue virus detection in Aedes aegypti larvae from southeastern Brazil

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