Molecular phylogeny of the Myzorhynchella Section of Anopheles (Nyssorhynchus) (Diptera: Culicidae): genetic support for recently described and resurrected species

Bourke, Brian P.; Nagaki, Sandra Sayuri; Bergo, Eduardo Sterlino; Cardoso, Jáder da Cruz; Sallum, Maria Anice Mureb

doi:10.1590/s0074-02762011000600010

Cited by 11 publications

(8 citation statements)

References 35 publications

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“…pristinus was described by Nagaki and Sallum (in Nagaki et al 2010, [41]). Recently, Bourke et al (2011) [54] suggested that individuals identified as An. lutzii A325 and An.…”

Section: Resultsmentioning

confidence: 99%

Phylogenetic Analysis and DNA-based Species Confirmation in Anopheles (Nyssorhynchus)

et al. 2013

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Specimens of neotropical Anopheles (Nyssorhynchus) were collected and identified morphologically. We amplified three genes for phylogenetic analysis–the single copy nuclear white and CAD genes, and the COI barcode region. Since we had multiple specimens for most species we were able to test how well the single or combined genes were able to corroborate morphologically defined species by placing the species into exclusive groups. We found that single genes, including the COI barcode region, were poor at confirming species, but that the three genes combined were able to do so much better. This has implications for species identification, species delimitation, and species discovery, and we caution that single genes are not enough. Higher level groupings were partially resolved with some well-supported groupings, whereas others were found to be either polyphyletic or paraphyletic. There were examples of known groups, such as the Myzorhynchella Section, which were poorly supported with single genes but were well supported with combined genes. From this we can infer that more sequence data will be needed in order to show more higher-level groupings with good support. We got unambiguously good support (0.94–1.0 Bayesian posterior probability) from all DNA-based analyses for a grouping of An. dunhami with An. nuneztovari and An. goeldii, and because of this and because of morphological similarities we propose that An. dunhami be included in the Nuneztovari Complex. We obtained phylogenetic corroboration for new species which had been recognised by morphological differences; these will need to be formally described and named.

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“…pristinus was described by Nagaki and Sallum (in Nagaki et al 2010, [41]). Recently, Bourke et al (2011) [54] suggested that individuals identified as An. lutzii A325 and An.…”

Section: Resultsmentioning

confidence: 99%

Phylogenetic Analysis and DNA-based Species Confirmation in Anopheles (Nyssorhynchus)

et al. 2013

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“…You can freely access the chapter at the Web Viewer here. Subgenus Kerteszia Morphology [44] Subgenus Nyssorhynchus ITS2 [159] Albimanus Section Morphology [24] Argyritarsis Section Morphology [25] Myzorhynchella Section ITS2, COI, white [160] Anopheles mosquitoes -New insights into malaria vectors 40…”

Section: Subgenus Anophelesmentioning

confidence: 99%

The Phylogeny and Classification of Anopheles

Harbach¹

2013

Anopheles Mosquitoes - New Insights Into Malaria Vectors

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Please use Adobe Acrobat Reader to read this book chapter for free. Just open this same document with Adobe Reader. If you do not have it, you can download it here. You can freely access the chapter at the Web Viewer here. 1 Geological ages of eras and periods follow the geological timescale determined by the International Commission on Stratigraphy (http://www.stratigraphy.org). Anopheles mosquitoes-New insights into malaria vectors 8 Please use Adobe Acrobat Reader to read this book chapter for free. Just open this same document with Adobe Reader. If you do not have it, you can download it here. You can freely access the chapter at the Web Viewer here.

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“…The chloroplast gene ndhF has about twice as many . Many studies have used this gene to reconstruct phylogenies or as a molecular marker within medicinal plant families [7][8][9][10][11][12][13] . A study about Bang Gi was reported by Sano et al that compared Boi (Sinomeni Caulis et Rhizoma) and Seifuto (Caulis Sinomenii) using internal transcribed spacer region sequence analysis 14) .…”

Section: Introduction 1)mentioning

confidence: 99%

Genetic identification of Sinomenium acutum based on chloroplast gene ndhF sequences

Ryuk¹,

Lee²,

Ko³

2013

The Korea Journal of Herbology

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Objectives : This study was conducted to identify the original Sinomini Caulis et Rhizoma plant among Stephania tetrandra, Cocculus trilobus, and Aristolochiae fangchi to develop the genetic marker for Sinomini Caulis et Rhizoma. Methods : Sinomenium acutum was identified by the classification and identification committee of the National Center for Standardization of Herbal Medicines. The chloroplast ndhF gene was amplified. We performed sequences alignment analysis of Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi using BioEdit program. The SFR markers designed were consisted of SF01, SR04, and SR05 primers. Results : Many variations of Sinomeni Caulis et Rhizoma are currently commercialized as herbal medicine. We compared the base sequences of the ndhF intergenic space of chloroplast DNA with Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi. According to the results, it showed that the nucleotide variations were seen in 30 genes of four species. Phylogenetic analysis revealed that 4 species were classified into five groups based on an inter-group divergence in nucleotide sequence of 9%. We developed SFR marker nucleotides enough to authenticate respective species and confirmed its application on the band size at 419 base pair. These sequence differences at corresponding positions were available genetic markers to identity the Sinomeni Caulis et Rhizoma. Conclusions : Base on these results, the ndhF region was effective in distinguishing Sinomini Caulis et Rhizoma The SFR genetic marker was useful for identifying Sinomini Caulis et Rhizoma with other species.

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Molecular phylogeny of the Myzorhynchella Section of Anopheles (Nyssorhynchus) (Diptera: Culicidae): genetic support for recently described and resurrected species

Abstract: Phylogenetic relationships among species of the Myzorhynchella Section of

Cited by 11 publications

References 35 publications

Phylogenetic Analysis and DNA-based Species Confirmation in Anopheles (Nyssorhynchus)

Phylogenetic Analysis and DNA-based Species Confirmation in Anopheles (Nyssorhynchus)

The Phylogeny and Classification of Anopheles

Genetic identification of Sinomenium acutum based on chloroplast gene ndhF sequences

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