2011
DOI: 10.1590/s0074-02762011000400021
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: In the current study, we evaluated the mechanism of action of miltefosine, which is the first effective and safe oral treatment for visceral leishmaniasis, in Leishmania amazonensis promastigotes. Miltefosine induced a process of programmed cell death, which was determined by the externalization of phosphatidylserine, the incorporation of propidium iodide, cell-cycle arrest at the sub-G0/G1 phase and DNA fragmentation into oligonucleosome-sized fragments. Despite the intrinsic variation that is detected in Lei… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

0
18
0
1

Year Published

2013
2013
2023
2023

Publication Types

Select...
5
3

Relationship

1
7

Authors

Journals

citations
Cited by 42 publications
(19 citation statements)
references
References 13 publications
0
18
0
1
Order By: Relevance
“…Non-treated cells were Annexin-V- and PI-negative (Figure 4), and the same result was seen in cells treated only with DMSO, the vehicle of drug (data not shown), which confirms the viability of cells in these conditions. Alternatively, promastigotes treated with miltefosine were used as a positive control in this experiment [23], [24]. After 24 h of incubation with miltefosine at 40 µM, 1.02% of cells were apoptotic-like (Annexin-V-positive and PI-negative) and 81.8% of cells were already in late apoptotic-like phase or necrosis (Annexin-V-positive and PI-positive) (Figure 4).…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…Non-treated cells were Annexin-V- and PI-negative (Figure 4), and the same result was seen in cells treated only with DMSO, the vehicle of drug (data not shown), which confirms the viability of cells in these conditions. Alternatively, promastigotes treated with miltefosine were used as a positive control in this experiment [23], [24]. After 24 h of incubation with miltefosine at 40 µM, 1.02% of cells were apoptotic-like (Annexin-V-positive and PI-negative) and 81.8% of cells were already in late apoptotic-like phase or necrosis (Annexin-V-positive and PI-positive) (Figure 4).…”
Section: Resultsmentioning
confidence: 99%
“…The intensity of labeling of Annexin-V conjugated to Alexa-Fluor was recorded in a FACSCalibur (BD Biosciences) flow cytometer and analyzed with Summit software, and the percentage of positive cells was assessed for each histogram. Miltefosine, an established inducer of apoptosis in L. amazonensis promastigotes (at 40 µM for 24 h) was used as a positive control [23], [24].…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Miltefosine is an inhibitor of Akt in Leishmania (15), and perifosine also inhibits Akt protein in human cancer cells (16). These facts lead us to believe that perifosine has the same Akt-inhibiting capacity in Leishmania, which leads to the induction of an apoptosis-like cell death that is known to occur with miltefosine in L. amazonensis (17) and L. donovani (18).…”
mentioning
confidence: 99%
“…It is known that both miltefosine and Sb III can induce programmed cell death (PCD) or necrosis (43)(44)(45)(46). We used propidium iodide (PI) and annexin V to stain promastigotes after miltefosine or Sb III exposure.…”
Section: (D) Ultrastructure Of the Flagellar Pocket Of L Donovani Prmentioning
confidence: 99%