In vitro effects of citral on Trypanosoma cruzi metacyclogenesis

Cardoso, Josiane; Soares, Maurílio J.

doi:10.1590/s0074-02762010000800012

Cited by 43 publications

(29 citation statements)

References 42 publications

(53 reference statements)

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“…Third, secondary metabolites could alter metacyclogenesis, a critical process by which trypanosomes transition between amastigote and choanomastigote life stages [45]. Indeed, some efforts to combat Chagas Disease focus on the chemical interruption of metacyclogenesis [46]. Additional research is needed to identify the mechanisms responsible for the reduction of Crithidia infection by secondary metabolite ingestion by bees.…”

Section: Discussionmentioning

confidence: 99%

Secondary metabolites in floral nectar reduce parasite infections in bumblebees

et al. 2015

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The synthesis of secondary metabolites is a hallmark of plant defence against herbivores. These compounds may be detrimental to consumers, but can also protect herbivores against parasites. Floral nectar commonly contains secondary metabolites, but little is known about the impacts of nectar chemistry on pollinators, including bees. We hypothesized that nectar secondary metabolites could reduce bee parasite infection. We inoculated individual bumblebees with Crithidia bombi, an intestinal parasite, and tested effects of eight naturally occurring nectar chemicals on parasite population growth. Secondary metabolites strongly reduced parasite load, with significant effects of alkaloids, terpenoids and iridoid glycosides ranging from 61 to 81%. Using microcolonies, we also investigated costs and benefits of consuming anabasine, the compound with the strongest effect on parasites, in infected and uninfected bees. Anabasine increased time to egg laying, and Crithidia reduced bee survival. However, anabasine consumption did not mitigate the negative effects of Crithidia, and Crithidia infection did not alter anabasine consumption. Our novel results highlight that although secondary metabolites may not rescue survival in infected bees, they may play a vital role in mediating Crithidia transmission within and between colonies by reducing Crithidia infection intensities.

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Section: Discussionmentioning

confidence: 99%

Secondary metabolites in floral nectar reduce parasite infections in bumblebees

et al. 2015

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“…Esto sería posible porque se ha demostrado in vitro que el citral en concentración mayor de 60 µg/mL produce 100% de lisis de formas epimastigotes y tripomastigotes de T. cruzi (30) .…”

Section: Discussionunclassified

Efecto del aceite esencial de Aloysia triphylla britton (cedrón) sobre el Trypanosoma cruzi en ratones

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2014

Rev Peru Med Exp Salud Publica

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RESUMENObjetivos. Determinar la actividad anti-Trypanosoma cruzi in vivo del aceite esencial de Aloysia triphylla en ratones. Materiales y Métodos. Los animales fueron asignados aleatoriamente a los siguientes grupos (n = 15 por grupo): infectados y no tratados (G1), infectados y tratados con benznidazol 100 mg/kg (G2), infectados y tratados con aceite esencial de Aloysia triphylla 100 mg/kg (G3), infectados y tratados con aceite esencial de Aloysia triphylla 250 mg/kg (G4); no infectados y no tratados (G5), y no infectados y tratados con 250 mg/kg de Aloysia triphyla (G6). La infección con T. cruzi se realizó con 10 4 tripomastigotes sanguíneos y el tratamiento empezó en el octavo día postinfección (dpi) hasta el 28 dpi. La parasitemia se determinó con microscopía óptica cada dos días en 5 µL de sangre extraída de la cola. En el 14, 21 y 28 dpi se obtuvo sangre de la cola para el ensayo de creatina kinasa-MB (CK-MB), alanina aminotransferasa y creatinina; después, los animales fueron sacrificados y se extrajo el corazón para el estudio histopatológico. Resultados. El aceite esencial de cedrón produjo una reducción significativa de 85,4% del pico de parasitemia con la dosis de 250 mg/kg; también produjo reducción del número de amastigotes e infiltrados inflamatorios en el corazón. El nivel plasmático de CK-MB también disminuyó en el 28 dpi por efecto de dicho tratamiento. Conclusiones. En condiciones experimentales, el aceite esencial de Aloysia triphylla tiene efecto anti-Trypanosoma cruzi in vivo en ratones. Palabras clave: Trypanosoma cruzi; Cedrón; Aceites esenciales; Experimentación animal (fuente: DeCS BIREME). THE EFFECT OF THE ESSENTIAL OIL FROM Aloysia triphylla BRITTON (LEMON VERBENA) ON Trypanosoma cruzi IN MICE ABSTRACTObjectives. To determine the in-vivo anti-Trypanosoma cruzi activity of the essential oil from Aloysia triphylla in mice. Materials and methods. The mice (n = 15) in the study were randomly assigned to the following groups: infected and untreated (G1), infected and treated with benznidazole 100 mg/kg (G2), infected and treated with of Aloysia triphylla essential oil 100 mg/kg (G3), infected and treated with of Aloysia triphylla essential oil 250 mg/kg (G4), uninfected and untreated (G5), and uninfected and treated with 250 mg/kg of Aloysia triphyla (G6). The infection was conducted using 104 blood T. cruzi trypomastigotes and the treatment began on the 8th day post-infection (dpi) through 28 dpi. The parasitemia was determined through optical microscopy every 2 days using 5 µL of blood sampled from the tail. In dpi 14, 21 and 28, the blood sample was obtained from the queue for testing creatine kinase-MB, alanine aminotransferase and creatinine, then animals were euthanized and the heart was removed for histopathological study. Results. The essential oil of lemon verbena produced a significant reduction of the parasitemia, 85.4% with the peak at a the dose of 250 mg/ kg. It was also observed a reduction in the number of amastigotes and inflammatory infiltrates in the heart. The creatin...

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“…Along these lines, the essential oil from C. cajucara could be a useful source of novel drugs. T. cruzi is also susceptible to the action of the Cymbopogom citratus essential oil (Santoro et al, 2007a;Cardoso & Soares, 2010). The essential oil is effective in killing T. cruzi with a low 50% inhibitory concentration (IC 50 ) at 15 µg/mL for bloodstream trypomastigotes.…”

Section: Essential Oils: Mode Of Action Against Trypanosoma Cruzimentioning

confidence: 99%

“…In addition, the treatment of T. cruzi with citral ( Figure 10), the main constituent of C. citratus essential oil, results in epimastigote growth inhibition and decrease of viable trypomastigotes at 42 and 14.2 μg/mL, respectively, showing its high microbicidal activity (Santoro et al, 2007a). This monoterpene also exerts inhibitory effects on T. cruzi metacyclogenesis (Cardoso & Soares, 2010). Although no effect is observed at concentrations lower than 20 μg/mL, metacyclogenesis is almost totally abolished at 40 μg/mL after 24, 48 or 72 h of incubation in TAU3AAG (differentiation) medium.…”

Section: Essential Oils: Mode Of Action Against Trypanosoma Cruzimentioning

confidence: 99%