2010
DOI: 10.1590/s0074-02762010000700018
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Abstract: Real-time PCR for Toxoplasma in chicken • Cleiton Paulo Aigner et al.

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Cited by 23 publications
(15 citation statements)
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“…Although it is not possible to confirm the viability of the parasite using PCR testing, the results call into question whether the consumption of this type of meat could be a risk to the consumer, particularly if the meat or viscera are undercooked (MILLAR et al, 2012). Yan et al (2010) studied chickens in China and reported that the organs with the greatest quantity of T. gondii parasites were the heart and lungs, and Aigner et al (2010) demonstrated the amplification of T. gondii DNA in chicken brain and heart samples in Paraná (Brazil). The present study also confirmed positivity in lung and heart samples.…”
Section: Discussionmentioning
confidence: 99%
“…Although it is not possible to confirm the viability of the parasite using PCR testing, the results call into question whether the consumption of this type of meat could be a risk to the consumer, particularly if the meat or viscera are undercooked (MILLAR et al, 2012). Yan et al (2010) studied chickens in China and reported that the organs with the greatest quantity of T. gondii parasites were the heart and lungs, and Aigner et al (2010) demonstrated the amplification of T. gondii DNA in chicken brain and heart samples in Paraná (Brazil). The present study also confirmed positivity in lung and heart samples.…”
Section: Discussionmentioning
confidence: 99%
“…Espírito Santo of the strains prevalent in the environment, as FR chickens become infected mostly by feeding from soil contaminated with oocysts (Ruiz and Frenkel, 1980). In the last 12 years we and others have determined prevalence of T. gondii in FR chickens from different regions of Brazil (Peixoto and Lopes, 1990;Garcia et al, 2000;Dubey et al, 2002;da Silva et al, 2003;Dubey et al, 2003a,b;Brandão et al, 2006;Dubey et al, 2006Dubey et al, , 2007Aigner et al, 2010;de Oliveira et al, 2009;Soares et al, 2011).…”
Section: Introductionmentioning
confidence: 99%
“…Despite high levels of antibodies to be associated with higher levels of the parasite in tissues, mainly in the brain (Aigner et al, 2010), probably was not obtained isolating in these mice because there were few bradyzoites in the aliquot used what may have led to not capture these cells in DNA extraction and subsequent ampliϐication in PCR. Even if the parasite DNA was extracted from this suspension, the same may not have been captured in the sample pipetted.…”
Section: Resultsmentioning
confidence: 99%