Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells

Andrade, Aron José Pazin de; Brasil, Bruno S. A. F.; Pereira, Anna C. T. C.; Ferreira, Paulo César Peregrino; Kroon, Erna Geessien; Bonjardim, Cláudio Antônio

doi:10.1590/s0074-02762010000300005

Cited by 2 publications

(1 citation statement)

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“…It is highly probable that radiospecificity will be impaired in infected individuals since stimulation of PBMC using phytohaemagglutinin stimulates p21 expression in vitro [1]. On the other hand, infections may also induce expression and activation of ATF-2, c-Myc, and CREB [13][14][15][16][17][18]. These markers could possibly help to reduce the risk of false p21 positivity in infected subjects.…”

Section: Discussionmentioning

confidence: 99%

EXPRESSION OF p21 AND EXPRESSION AND ACTIVATION OF MAPK REGULATED TRANSCRIPTION FACTORS IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN RATS AFTER WHOLE-BODY γ-RADIATION AND ITS USE IN BIODOSIMETRY

Pejchal¹,

Průchová²,

Kubelková³

et al. 2016

Mil. Med. Sci. Lett.

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The aim of our study was to examine the in vivo expression of p21 and expression and activation of ATF-2, c-Myc, and CREB in rat peripheral blood mononuclear cells (PBMC) after whole body γ-irradiation and to assess its contribution to biodosimetry. For Western blot experiments, male Wistar rats were whole-body irradiated by a single dose of 0, 0.5, 1, 3, and 5 Gy (60 Co, 1 m, 0.7 Gy/min). As a positive control, leukaemic MOLT-4 cells were used. For ELISA experiments, male Wistar rats were whole-body irradiated by a single dose of 0, 1, 2, 3, 4, and 5 Gy (60 Co, 1 m, 0.6 Gy/min). Blood samples were taken 4 h after the irradiation and PBMC were isolated using centrifugation on Histopaque-1077. Expressions of p21, ATF-2, phospho-ATF-2 Thr69/71 , c-Myc, phospho-c-Myc Thr-58/Ser62 , CREB, and phospho-CREB Ser133 were measured using Western blot method. Expression of p21 was also quantified using ELISA. We observed increase of p21 expression in rat PBMC 4 h after irradiation. According to ELISA, p21 levels increased 2.0-, 3.1-, 5.5-, 3.0-, and 3.1fold after irradiation by 1, 2, 3, 4, and 5 Gy, respectively. We did not detect any expression or activation of ATF-2, c-Myc and CREB. Protein p21 could be considered as a perspective biodosimetric marker of clinically significant irradiation (≥1 Gy) in vivo in unstimulated PBMC.

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