α-N-acetylglucosamine-linked O-glycans of sialoglycoproteins (Tc-mucins) from Trypanosoma cruzi Colombiana strain

Abstract

Trypanosoma cruzi sialoglycoproteins (Tc-mucins)

Intraspecies Variation in Trypanosoma cruzi GPI-Mucins: Biological Activities and Differential Expression of α-Galactosyl Residues

“…15,51,52 A number of publications described intraspecies polymorphisms in the O-glycosilations of GPImucins from different T. cruzi strains (G, Colombiana, CLBrener, and Y). 15,[22][23][24][25]53 Extensive studies have reported on the biological activities of GPI-mucins from Y strain trypomastigotes, especially in the induction of NO and proinflammatory cytokines (IL-12 and TNF-α) by macrophages. 16,31,37 Furthermore, the molecular mechanisms of this induction were reported to involve members of the nuclear factor κB (NF-κB) pathway, including TLR2/TLR6, 17,18 MyD88, 54 extracellular signal-related (ERK) -1/ERK2 kinases, mitogen-activated protein kinase (MAPK), stress-activated protein kinase (SAPK-2/p38), and inhibitor of κB (IκB).…”

“…Recently, it was proposed to use structural data for taxonomic purposes for comparing the O-glycans of T. cruzi I and II lineages. 25,71 Here, we could also discriminate all lineages in T. cruzi I, II, and VI using the newly reported consensus. 26 We could not correlate those variations to the BZ resistance phenotype, and they are probably strain-specific.…”

“…These antibodies have protective lytic activity against trypomastigotes and are determinant in both acute and chronic phases of Chagas disease. 15,21 The O-linked sugar structures in the GPI-mucins have already been fully characterized in epimastigotes and metacyclic stages of Colombiana, Y, and CL [22][23][24][25] but only partially defined in trypomastigotes of the Y strain. 15 Currently, after a consensus, the molecular studies enabled the classification of T. cruzi strains into six (TcITcVI) discrete typing units (DTUs).…”

Expanding the knowledge of the chemical structure of glycoconjugates from Trypanosoma cruzi TcI genotype. Contribution to taxonomic studies

“…The HSQC spectra of all five alditols (Figures 2-6) showed the expected pattern for a furanose ring with C 4 being strongly deshielded and also confirmed the substitution positions hinted at by the ROESy experiments. The chemical shifts observed for these samples were compared with the ones found for the Colombian, Dm28 and G strains (Previato et al 1994, Todeschini et al 2009, Agrellos et al 2003). Galp-β-1-6-GlcNAc-ol; 3.…”

Sialic acid: a sweet swing between mammalian host and Trypanosoma cruzi

“…The structure of the oligosaccharides O -linked to the mucins of the non-infective epimastigote forms have been described (Previato et al, 1994, 1995; Todeschini et al, 2001, 2009; Agrellos et al, 2003; Jones et al, 2004). These O -glycans differ from those found in mammalian systems in three main aspects: (i) they are linked to the peptide backbone through an α- N -acetylglucosamine (α-GlcNAc) residue (Previato et al, 1998), rather than an α- N -acetylgalactosamine (α-GalNAc; Gill et al, 2011); (ii) they are further substituted by (Gal) on O -4 and O -6 rather than O -3 and O -6 as found in mammalian mucins (Varki et al, 2009); (iii) several strains carry a β-galactofuranose (β-Gal f ) attached to the GlcNAc O -4 (Previato et al, 1994; Agrellos et al, 2003; Jones et al, 2004).…”