Experimental reinfection of BALB/c mice with different recombinant type I/III strains of Toxoplasma gondii: involvement of IFN-³ and IL-10

Abstract

To assess reinfection of BALB/c mice with different

IL10, TGF Beta1, and IFN Gamma Modulate Intracellular Signaling Pathways and Cytokine Production to Control Toxoplasma gondii Infection in BeWo Trophoblast Cells1

“…Furthermore, IL10 and STAT3 are involved in the increased susceptibility to infections caused by Leishmania amazonensis, Leishmania donovani, the Newcastle disease virus, and Listeria monocytogenes [45][46][47][48]. According to our present data, IL10 also promoted higher infectivity during T. gondii infection in murine dendritic cells [49], and increased IL10 levels were associated with the high susceptibility to reinfection with different recombinant T. gondii strains in BALB/c mice [50]. Thus, IL10 and STAT3 are involved in the higher susceptibility to many types of infection, including T. gondii in the maternal-fetal interface, and our present findings are the first to show this type of effect in a human trophoblast model.…”

“…For this purpose, BeWo cells were cultured in 96-well plates (2 3 10 4 cells/well/200 ll) in RPMI 1640 medium with 10% FBS at 378C and 5% CO 2 for 24 h. Next, cells were infected with T. gondii tachyzoites (5:1) in RPMI 1640 medium with 2% FBS, and, after 3 h, cells were washed to remove extracellular parasites and treated with increasing concentrations (20,50,100,150, and 200 ng/ml) of rIFNG (Invitrogen Life Technologies, Carlsbad, CA) for an additional 24 h in RPMI 1640 medium with 2% FBS. Cells were analyzed for T. gondii intracellular proliferation using a colorimetric betagalactosidase assay as previously described [19].…”

Toxoplasma gondii: epidemiology, feline clinical aspects, and prevention

“…It is thought that infection to one genotype of T. gondii confers immunity to all genotypes, but this phenomenon has not been explored in cats. In a mouse model, immunity was more effective using homologous rather than heterologous strains of the parasite [7,8].…”

Pathological changes in acute experimental toxoplasmosis with Toxoplasma gondii strains obtained from human cases of congenital disease

“…For experimental infections, BALB/c and C57BL/6 mice received 10 cysts in a volume of 0.2 ml orally. The survivors at 30 dpi had serum samples tested for anti-T. gondii IgG using ELISA according to Brandão et al (2009). BALB/c and C57BL/6 non-infected mice were used as controls in all experiments.…”