Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia

Cristiano, Fabio Aníbal; Pérez, Manuel A.; Nicholls, Rubén Santiago; Guerra, Ángela Patricia

doi:10.1590/s0074-02762008000500015

Cited by 19 publications

(20 citation statements)

References 17 publications

(15 reference statements)

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“…However, we could identify approximately seven haplotypes, which were previously described, including the C haplotype, which has the same digestion profile in all previous studies. When comparing the fragment profiles, the haplotypes similar to those described here were as follows: one from Colombia (Cristiano et al 2008), three from Peru (Sutton et al 2009), four from PNG (Bruce et al 2000) and six from Thailand (Cui et al 2003). These findings suggest a global distribution of parasites containing similar PvMSP-3α genotypes.…”

Section: Discussionsupporting

confidence: 73%

“…However, when taking into account only American isolates, this frequency decreased to 22%. When comparing the PvMSP-3α gene variability of Brazilian isolates with isolates solely from other South America locations (Peru, Venezuela, Colombia and French Guiana), the prevalence of PvMSP-3α gene types were more similar to those reported in Venezuela (Ord et al 2005) and were more distinct from data collected in Colombia (Cristiano et al 2008). In two localities (India and Pakistan), a rare type D allele (0.3-0.5 kb) was identified.…”

Section: Discussionmentioning

confidence: 56%

“…The total number of haplotypes was similar in Brazil (n = 11, herein), French Guiana (n = 11) (Veron et al 2009), Colombia (n = 9) (Cristiano et al 2008) and Venezuela (n = 9) (Ord et al 2005), but it was TABLE II Analysis of genetic diversity and population differentiation of Plasmodium vivax isolates from different Brazilian Amazon regions using polymerase chain reaction-restriction fragment length polymorphism of P. vivax merozoite surface protein-3αlpha…”

Section: Discussionmentioning

confidence: 95%

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Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates

Ribeiro¹,

Ladeira²,

Rezende

et al. 2011

Mem. Inst. Oswaldo Cruz

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 95%

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Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates

Ribeiro¹,

Ladeira²,

Rezende

et al. 2011

Mem. Inst. Oswaldo Cruz

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“…Some allele types of Alu I digestion are comparable to those reported in other parts of the world, such as allele types A2, A3, A4, and A5 isolated in Anhui Province of China [35], types A1, A2, A3, A4, A5, and A8 in Pakistan [11], types A1, A2, A3, A4, and A5 in Iran [37], and types A1, A2, and A5 in Colombia [38], which suggests that these allele types of P. vivax may have a global distribution. The allele variant B1 was a new allele identified in the present study.…”

Section: Discussionmentioning

confidence: 77%

Genetic diversity of Plasmodium vivax population before elimination of malaria in Hainan Province, China

Wang

Feng

et al. 2015

Malar J

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BackgroundHainan Province is one of the most severe endemic regions with high transmission of Plasmodium falciparum and Plasmodium vivax in China. However, the incidence of P. falciparum and P. vivax has dropped dramatically since 2007 and a national elimination malaria programme (NEMP) was launched after 2010. To better understand the genetic information on P. vivax population before elimination of malaria in Hainan Province, the extent of genetic diversity of P. vivax isolates in Hainan Province was investigated using four polymorphic genetic markers, including P. vivax merozoite surface proteins 1, 3α, and 3β (pvmsp-1, pvmsp-3α, and pvmsp-3β) and circumsporozoite protein (pvcsp).MethodsIsolates of P. vivax (n = 27) from Hainan Province were collected from 2009 to 2010 and pvmsp-1 and pvcsp were analysed by DNA sequencing, respectively. Using polymerase chain reaction-restriction fragment length polymorphism were analysed in pvmsp-3α, and pvmsp-3β.ResultsThe DNA sequencing analysis on pvmsp1 revealed that there were three allele types: Salvador-1 (Sal-1), Belem and recombinant (R) types. Among them, Sal-1 type was a dominant strain with eight variant subtypes (88.9%), whereas R- (3.7%) and Belem-type strains (7.4%) had one variant subtypes, respectively. All the isolates carried pvcsp with VK210 type accounting for 85.2% (23/27 isolates) and VK247 type accounting for 14.8% (4/27). Only type A and type B alleles were successfully amplified in pvmsp-3α gene, and a high level of polymorphism was observed in pvmsp-3α. Considering pvmsp-3β gene, type A was the predominant type in 17 isolates (63%), whereas type B was dominant in only ten isolates (37%).ConclusionThe present data indicate that there was high degree of genetic diversity among P. vivax population in Hainan Province of China during the pre-elimination stage of malaria, with 26 unique haplotypes observed among 27 samples.

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“…The gene coding for the MSP-3α (PvMSP-3α) is highly polymorphic and its variability has been assessed by restriction fragment length polymorphism-PCR analysis (Bruce et al 1999). The suitability of PvMSP-3α as a molecular marker has been confirmed in several geographic parasite populations: Papua New Guinea (Bruce et al 1999), Thailand (Cui et al 2003b, Mascorro et al 2005, Pakistan and Iran (Zakeri et al 2006, Khatoon et al 2010, Venezuela (Ord et al 2005), Peru (Sutton et al 2009), Colombia (Cristiano et al 2008), India (Prajapati et al 2010) and French Guiana (Veron et al 2009). However, classifying restriction fragments according to size after electrophoresis is relatively subjective, which hampers large-scale analyses and reduces comparisons between studies.…”

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confidence: 96%

Molecular markers and genetic diversity of Plasmodium vivax

Brito

Ferreira

2011

Mem. Inst. Oswaldo Cruz

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Enhanced understanding of the transmission dynamics and population genetics for Key words: malaria -Plasmodium vivax -genetic markers -genetic diversity -multiple-clone infections -microsatellitesMarkers and genetic diversity of P. vivax • Cristiana Ferreira Alves de Brito, Marcelo Urbano Ferreira 13 tual heterozygosity (H E ) values. Virtual H E is the average probability that a pair of alleles randomly obtained from the population is different. Whenever appropriate, in this review we provide H E estimates obtained using different markers for different populations.Molecular markers under selection -Until recently, most genetic markers available for characterising natural P. vivax populations were orthologues of previously identified P. falciparum antigen-coding genes. The wellcharacterised polymorphic regions in these genes have been extensively used to analyse genetic diversity patterns in P. falciparum (Roy et al. 2008). A similar approach has been explored in vivax-oriented studies (Cui et al. 2003a). The following single-copy genes in P. vivax have often been used in these studies: gam1, coding for the gametocyte antigen 1, csp, coding for the circumsporozoite protein (CSP), msp1 and msp3alpha, coding for the merozoite surface proteins (MSP)-1 and 3α, respectively, ama1, coding for the apical membrane antigen 1, and dbp, coding for the Duffy binding protein (DBP) ( Table I).A notable feature of several P. vivax surface antigens (including major vaccine-candidate molecules) is tandem arrays of relatively short amino acid motifs. The CSP in P. vivax (PvCSP), an abundant antigen on the surface of sporozoites that has been extensively used as a vaccine development target, has immunodominant B-cell epitopes that map to central repeats between nonrepetitive sequences (Nardin & Zavala 1998). PvCSP displays two major types of nonapeptide repeats [most commonly GDRA(D/A)GQPA and ANGA(G/D)(N/D)QPG], which define the variants known as VK210 and VK247, respectively (Arnot et al. 1985, Rosenberg et al. 1989 (Fig. 1). Both VK210 and VK247 variants are globally distributed, but geographic biases have been described (Cochrane et al. 1990, Qari et al. 1993a. Similar repetitive sequences are found in Brazil, Southeast Asia and Papua New Guinea (Qari et al. 1991(Qari et al. , 1992. However, markedly divergent sequence variants were found in isolates from China and North Korea (Mann et al. 1994). Although VK210 and VK247-type sequences often occur in sympatric parasite populations, there are no examples of a hybrid repeat array with both repeat types (Lim et al. 2005).A third type of repeat unit [APGANQ(E/G)GAA], identical to that described for Plasmodium simiovale, characterises the so-called P. vivax-like parasites (Qari et al. 1993a). Although P. vivax-like CSP repeats have been found in parasite isolates across the world, its global distribution remains unconfirmed (Gopinath et al. 1994). In Brazil, P. vivax-like parasites have been identified only in mixed-clone infections with VK210-type or VK247-type parasites (Machado...

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Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia

Cited by 19 publications

References 17 publications

Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates

Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates

Genetic diversity of Plasmodium vivax population before elimination of malaria in Hainan Province, China

Molecular markers and genetic diversity of Plasmodium vivax

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