In vitro and in vivo activity of meglumine antimoniate produced at Farmanguinhos-Fiocruz, Brazil, against Leishmania (Leishmania) amazonensis, L (L.) chagasi and L (Viannia) braziliensis

Morais-Teixeira, Eliane de; Carvalho, Alcione Silva de; Costa, Jorge; Duarte, Silvio Luiz; Mendonça, Jorge S.; Boechat, Núbia; Rabello, Ana

doi:10.1590/s0074-02762008000400008

Cited by 27 publications

(17 citation statements)

References 12 publications

(10 reference statements)

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“…The lower susceptibility of L. chagasi is frequently found also with respect to other drugs (Morais-Teixeira et al, 2008;Braga et al, 2007) including the standard drug Amphotericin B (Table 2). The mechanism of action by which EOLC inhibits the parasite growth is still unknown, however some authors (Wink, 2008;Cabral et al, 2009;Laouer et al, 2009) have shown the biological activity of the major compounds present in this EOLC against other eukaryotes parasites and prokaryote microorganisms, which are distant from Leishmania genus, as punctuated before, this work is the first one that demonstrates the activity of EOLC on promastigote culture of Leishmania, so it is relevant to consider some previous studies which enables the construction of few hypothesis to contribute for explanation of EOLC activity on this protozoa culture.…”

Section: Resultsmentioning

confidence: 95%

Essential oil from leaves of Lantana camara: a potential source of medicine against leishmaniasis

Machado

Valente²,

Lesche³

et al. 2012

Rev. bras. farmacogn.

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Abstract:Leishmaniasis is an infection of viscera or tegument caused by protozoa Leishmania sp. The extensive period required for the treatment, which involves the use of toxic medicines, leads patients to drop treatment increasing the development of resistant forms of Leishmania sp. Lantana camara L., Verbenaceae, is a tropical plant native from America. Folk uses have been described for treatment of tumors, tetanus, rheumatism and malaria. This study evaluates the leishmanicidal activity of the essential oil of leaves from L. camara on promastigote forms of Leishmania chagasi and L. amazonensis and its toxic effects on Artemia salina (brine shrimp test), macrophage cultures and BALB/c mice. The chemical composition was evaluated using the gas chromatography coupled with mass spectrometer (GC-MS). Thirty substances, mostly mono and sesquiterpenes were identifi ed. The most representative constituents were: germacrene D (24.90%), farnesene derivatives (22%) and (E)-cariophylene (14.31%). Bioassays revealed a signifi cant leishmanicidal activity of essential oil against L. amazonensis (IC50 0.25 μg/ mL) and a potential toxic effect on Brine shrimp (LC50 10 μg/mL) and macrophage assays (CC50 4 μg/mL), while there was no toxic manifestation on mice. The data show the relevant potential of L. camara as a source of medicine for leishmaniasis treatment.

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Section: Resultsmentioning

confidence: 95%

Essential oil from leaves of Lantana camara: a potential source of medicine against leishmaniasis

Machado

Valente²,

Lesche³

et al. 2012

Rev. bras. farmacogn.

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show abstract

“…However, this is somewhat time consuming since the amastigotes must be isolated from infected animals. Alternatively, amastigotes may be obtained by in vitro transformation of promastigotes with minimal changes to the growth conditions and temperature [45]. This method provides effective drug susceptibility assays with persistent infection in macrophages.…”

Section: Classical Methodsmentioning

confidence: 99%

Current Approaches to Discover Marine Antileishmanial Natural Products

2011

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“…This experiment was carried out as described by Morais-Teixeira et al, with some modifications [56]. Hamsters were randomly split into two groups of six animals.…”

Section: Chronic Infection Model Of CL In Golden Hamstermentioning

confidence: 99%

Evaluating the Potential of Ursolic Acid as Bioproduct for Cutaneous and Visceral Leishmaniasis

et al. 2020

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Leishmaniasis affects around 12 million people worldwide and is estimated to cause the ninth-largest disease burden. There are three main forms of the disease, visceral (VL), cutaneous (CL), and mucocutaneous (MCL), leading to more than one million new cases every year and several thousand deaths. Current treatments based on chemically synthesized molecules are far from ideal. In this study, we have tested the in vitro and in vivo efficacy of ursolic acid (UA), a multifunctional triterpenoid with well-known antitumoral, antioxidant, and antimicrobial effects on different Leishmania strains. The in vitro antileishmanial activity against the intracellular forms was six and three-fold higher compared to extracellular forms of L. amazonensis and L. infantum, respectively. UA also showed to be a potent antileishmanial drug against both VL and CL manifestations of the disease in experimental models. UA parenterally administered at 5 mg/kg for seven days significantly reduced the parasite burden in liver and spleen not only in murine acute infection but also in a chronic-infection model against L. infantum. In addition, UA ointment (0.2%) topically administered for four weeks diminished (50%) lesion size progression in a chronic infection model of CL caused by L. amazonensis, which was much greater than the effect of UA formulated as an O/W emulsion. UA played a key role in the immunological response modulating the Th1 response. The exposure of Leishmania-infected macrophages to UA led to a significant different production in the cytokine levels depending on the Leishmania strain causing the infection. In conclusion, UA can be a promising therapy against both CL and VL.

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In vitro and in vivo activity of meglumine antimoniate produced at Farmanguinhos-Fiocruz, Brazil, against Leishmania (Leishmania) amazonensis, L (L.) chagasi and L (Viannia) braziliensis

Abstract: The leishmanicidal activity of four batches of meglumine antimoniate, produced in Farmanguinhos-Fiocruz, Brazil (TAMs)

Cited by 27 publications

References 12 publications

Essential oil from leaves of Lantana camara: a potential source of medicine against leishmaniasis

Essential oil from leaves of Lantana camara: a potential source of medicine against leishmaniasis

Current Approaches to Discover Marine Antileishmanial Natural Products

Evaluating the Potential of Ursolic Acid as Bioproduct for Cutaneous and Visceral Leishmaniasis

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