Antimicrobial resistance profiles of enterococci isolated from poultry meat and pasteurized milk in Rio de Janeiro, Brazil

Fracalanzza, Suely Aparecida Pimenta; Scheidegger, Erica Miranda Damásio; Santos, Patricia Faria dos; Leite, Paola Cardarelli; Teixeira, Lúcia Martins

doi:10.1590/s0074-02762007005000120

Cited by 42 publications

(41 citation statements)

References 28 publications

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“…The witness for this claim is the frequent studies in which the presence of resistance, high-level aminoglycoside resistance, and other antibiotic resistance genes have been indicated in human, animals and food resources (25)(26)(27)(28). Overall, there are scarce reports on aminoglycoside resistance genes in Salmonella species (13).…”

Section: Discussionmentioning

confidence: 99%

Study of Aminoglycoside Resistance Genes in Enterococcus and Salmonella Strains Isolated From Ilam and Milad Hospitals, Iran

Samadi

Pakzad

Sefidan

et al. 2015

Jundishapur J Microbiol

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Background: Aminoglycosides are a group of antibiotics that have been widely used in the treatment of life-threatening infections of Gram-negative bacteria. Objectives: This study aimed to evaluate the frequency of aminoglycoside resistance genes in Enterococcus and Salmonella strains isolated from clinical samples by PCR. Materials and Methods: In this study, 140 and 79 isolates of Enterococcus and Salmonella were collected, respectively. After phenotypic biochemical confirmation, 117 and 77 isolates were identified as Enterococcus and Salmonella, respectively. After the biochemical identification of the isolates, antibiotic susceptibility for screening of resistance was done using the Kirby-Bauer method for gentamicin, amikacin, kanamycin, tobramycin and netilmycin. DNA was extracted from resistant strains and the presence of acc (3)-Ia, aac (3′)-Ib, acc (6)-IIa ,16SrRNA methylase genes (armA and rat) was detected by PCR amplification using special primers and positive controls. Results: Enterococcus isolates have the highest prevalence of resistance to both kanamycin and amikacin (68.4%), and Salmonella isolates have the highest prevalence of resistance against kanamycin (6.9%). Ninety-three and 26 isolates of Enterococcus and Salmonella at least were resistant against one of the aminoglycosides, respectively. Moreover, 72.04%, 66.7%, and 36.6% of the resistant strains of Enterococcus had the aac (3′)-Ia, aac (3′)-IIa, and acc (6′)-Ib genes, respectively. None of the Salmonella isolates have the studied aminoglycoside genes. Conclusions: Our results indicate that acetylation genes have an important role in aminoglycoside resistance of the Enterococcus isolates from clinical samples. Moreover, Salmonella strains indicate very low level of aminoglycoside resistance, and aminoglycoside resistance genes were not found in Salmonella isolates. These results indicate that other resistance mechanisms, including efflux pumps have an important role in aminoglycoside resistance of Salmonella.

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Section: Discussionmentioning

confidence: 99%

Study of Aminoglycoside Resistance Genes in Enterococcus and Salmonella Strains Isolated From Ilam and Milad Hospitals, Iran

Samadi

Pakzad

Sefidan

et al. 2015

Jundishapur J Microbiol

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“…There is a growing interest for the characterization of enterococci isolated from food since microbial AMR genes present in retail meat or dairies could possibly be transferred to the consumer (17,23,39,43,47,49). Some AMR genes have been described in the literature as species characteristic and consequently have been used for their identification (5, 6).…”

Section: Discussionmentioning

confidence: 99%

“…Widespread use of antimicrobial agents in livestock and poultry has raised concerns that bacterial pathogens, having food animal reservoirs, could develop drug resistance and transfer these antimicrobial resistance (AMR) genes to humans through the consumption of retail meat (1,49,51). Consequently, there is an increasing interest in characterizing virulence factors and antibiotic resistance in enterococci causing nosocomial infections in hospitals or isolated from food products (17,23,25,39,43,44,47). Rapid and accurate identification of infection-related enterococcal species is crucial in choosing the appropriate treatment for patients (2).…”

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confidence: 99%

Development of a DNA Microarray for Enterococcal Species, Virulence, and Antibiotic Resistance Gene Determinations among Isolates from Poultry

Champagne

Diarra

Rempel

et al. 2011

Appl Environ Microbiol

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A DNA microarray (Enteroarray) was designed with probes targeting four species-specific taxonomic identifiers to discriminate among 18 different enterococcal species, while other probes were designed to identify 18 virulence factors and 174 antibiotic resistance genes. In total, 262 genes were utilized for rapid species identification of enterococcal isolates, while characterizing their virulence potential through the simultaneous identification of endogenous antibiotic resistance and virulence genes. Enterococcal isolates from broiler chicken farms were initially identified by using the API 20 Strep system, and the results were compared to those obtained with the taxonomic genes atpA, recA, pheS, and ddl represented on our microarray. Among the 171 isolates studied, five different enterococcal species were identified by using the API 20 Strep system: Enterococcus faecium, E. faecalis, E. durans, E. gallinarum, and E. avium. The Enteroarray detected the same species as API 20 Strep, as well as two more: E. casseliflavus and E. hirae. Species comparisons resulted in 15% (27 isolates) disagreement between the two methods among the five API 20 Strep identifiable species and 24% (42 isolates) disagreement when considering the seven Enteroarray identified species. The species specificity of key antibiotic and virulence genes identified by the Enteroarray were consistent with the literature adding further robustness to the redundant taxonomic probe data. Sequencing of the cpn60 gene further confirmed the complete accuracy of the microarray results. The new Enteroarray should prove to be a useful tool to accurately genotype strains of enterococci and assess their virulence potential.Enterococci are commensal bacteria found in the gastrointestinal tracts of humans, animals, and birds, as well as in soil and water. Sequenced Enterococcus faecalis and E. faecium genomes revealed the presence of virulence factors and mobile and/or exogenously acquired DNA giving some insight on how these bacteria made the transition from a commensal organism to a nosocomial pathogen (21,46,53). Enterococci can infect the endocardium, abdomen, urinary tract, burn or surgical wounds, and numerous other tissues, especially in immunocompromised patients (24, 59). E. faecalis causes the majority of infections, followed by E. faecium (53). Other enterococci such as E.

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“…Este resultado foi similar aos observados por Fracalanzza et al (2007) faecalis, E. faecium e Lactobacillus reuteri no trato digestivo de camundongos e transferido entre cepas de L. curvatus em salsichas fermentadas, corroborando com a hipótese de que Enterococcus resistentes podem contaminar os alimentos, entrar no trato gastrointestinal humano e colonizar os seres humanos e/ou passar seus genes de resistência para bactérias comensais presentes no trato intestinal humano, situação de risco para a saúde pública (Riboldi et al 2009). …”

Section: Resultados E Discussão Isolamento Microbiológico Em Amostrasunclassified

Resistência antimicrobiana em Enterococcus faecalis e Enterococcus faecium isolados de carcaças de frango

et al. 2013

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(B), vanC-1, aph(3')-llla, ant(6)-la, vanB, vanA, aac(6')--le-aph(2'')-la, erm(A) and tet(M) resistance genes, the last one most often verified. The results might suggest problems for public health due the high resistance, since these microorganisms have ability to transmit genes for antimicrobial resistance to others in the intestinal tract of humans and animals. Thus, the use of these drugs for clinical treatment could be hindered. (B), vanC-1, aph(3')-llla, ant(6)-la, vanB, vanA, aac(6')-le-aph(2'')-la, erm(A) e tet(M) -este último mais frequente. Estes resultados sugerem sérios problemas para a Saúde Pública, uma vez que esses microrganismos podem possuir a capacidade de transmitir genes de resistência antimicrobiana para outros microrganismos presentes na microbiota intestinal de humanos e animais, podendo inviabilizar o uso destas drogas para tratamentos clínicos.

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Antimicrobial resistance profiles of enterococci isolated from poultry meat and pasteurized milk in Rio de Janeiro, Brazil

Abstract: tetracycline, 23.8% to erythromycin, 11.3% to streptomycin, 4.3% to chloramphenicol, 3.9% to gentamicin, 1.4% to norfloxacin, 1.1% to imipenem, 0.7% to ciprofloxacin, nitrofurantoin, and penicillin, and 0.4%

Cited by 42 publications

References 28 publications

Study of Aminoglycoside Resistance Genes in Enterococcus and Salmonella Strains Isolated From Ilam and Milad Hospitals, Iran

Study of Aminoglycoside Resistance Genes in Enterococcus and Salmonella Strains Isolated From Ilam and Milad Hospitals, Iran

Development of a DNA Microarray for Enterococcal Species, Virulence, and Antibiotic Resistance Gene Determinations among Isolates from Poultry

Resistência antimicrobiana em Enterococcus faecalis e Enterococcus faecium isolados de carcaças de frango

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