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Cited by 61 publications
(50 citation statements)
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“…Real-time PCR (qPCR) has additional advantages over conventional PCR: it is able to detect lower concentrations of target DNA, it is quantitative, and it is less labor-intensive, since there is no need for electrophoresis to visualize products. The technique has been used for the identification of the different human schistosome species and in assessing infection intensity (197,209,210). An expansion on qPCR techniques is multiplex PCR, which amplifies more than one DNA target in a single reaction mixture and has been used successfully for case detection (197), for differentiating between S. japonicum, S. mansoni, and S. haematobium, and as an important tool in epidemiological studies and in monitoring schistosomiasis control programs (197,206).…”
Section: Detection Of Schistosoma Dna By Pcrmentioning
confidence: 99%
“…Real-time PCR (qPCR) has additional advantages over conventional PCR: it is able to detect lower concentrations of target DNA, it is quantitative, and it is less labor-intensive, since there is no need for electrophoresis to visualize products. The technique has been used for the identification of the different human schistosome species and in assessing infection intensity (197,209,210). An expansion on qPCR techniques is multiplex PCR, which amplifies more than one DNA target in a single reaction mixture and has been used successfully for case detection (197), for differentiating between S. japonicum, S. mansoni, and S. haematobium, and as an important tool in epidemiological studies and in monitoring schistosomiasis control programs (197,206).…”
Section: Detection Of Schistosoma Dna By Pcrmentioning
confidence: 99%
“…The detection limit was 3 fg of genomic DNA. Since the genome of S. mansoni contains ~580 fg of DNA, this is equivalent to less than a single cell of this multi-cellular parasite (Gomes et al 2006).…”
mentioning
confidence: 99%
“…These assays are performed with a single setup and dye detection within a closed tube, which decreases the risk of DNA contamination. The first method described for schistosomiasis diagnosis used SYBER Green dye for the detection of S. mansoni, targeting a small 96bp fragment on the small subunit-ribosomal ribonucleic acid (SSU rRNA) gene 89 . DNA extracts from adult S. mansoni worms were used in the system evaluation, showing a parasite DNA detection limit of 10fg.…”
mentioning
confidence: 99%