The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys

Trypanosoma (Duttonella) vivax: its biology, epidemiology, pathogenesis, and introduction in the New World - a review

“…Serological tests available for antibody detection have problems related to specificity due to cross-reaction with other parasites of the same genus, especially in endemic areas where T. evansi infections occur (Araújo et al 1997). The standardization and evaluation of an ELISA with the crude antigen of a Brazilian isolate of T. vivax and seroepidemiological surveys in cattle from PMS and in various micro regions in the PA, Brazil, have been conducted by Madruga et al (2006). In these studies, the sensitivity and specificity were 97.6% and 96.9%, respectively.…”

Primeiro registro de Trypanosoma vivax em bovinos no Estado do Rio Grande do Sul, Brasil

“…Até recentemente, os estudos sobre T. vivax no Brasil foram conduzidos somente em áreas endêmicas, principalmente após surtos de doença aguda, como ocorridos no Pantanal (SILVA et al, 1996;VENTURA et al, 2001;MADRUGA et al, 2006;CORTEZ et al, 2006). Porém, os relatos recentes de infecção por T. vivax em bovinos da Paraíba (BATISTA et al, 2007), Maranhão (GUERRA et al, 2008) (VENTURA et al, 2001;DAVILA et al, 2003;RODRIGUES et al, 2003;CORTEZ et al, 2009).…”

Trypanosoma vivax infection dynamics in a cattle herd maintained in a transition area between Pantanal lowlands and highlands of Mato Grosso do Sul, Brazil

“…The occurrence of Trypanosoma vivax was detected by Woo test (Woo 1969) and the specific antibodies were diagnosed through enzyme-linked immunosorbent assay (Tv-ELISA-Ab) developed by Madruga et al (2006). Both tests were used for selection and monitoring of the experimental groups.…”

“…Nevertheless, the first species does not show crossed reaction with T. vivax (Luckins 1977), but the latter Trypanosoma species has antigens that induce antibodies which display cross-reactions with T. vivax (Camargo et al 2004). However, the reduced level of cross-reactions in the ELISA test used with sera of animals infected with T. evansi (Madruga et al 2006) and the low prevalence of this haemoprotozoan in the Brazilian and Bolivian Pantanal (Franke et al 1994, Dávila et. al.…”