Molecular characterization of human Trypanosoma cruzi isolates from endemic areas in Panama

Sousa, Octavio E.; Samudio, Franklyn; Juncá, Corina de; Calzada, José E.

doi:10.1590/s0074-02762006000400018

Cited by 23 publications

(21 citation statements)

References 17 publications

(13 reference statements)

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“…A similar situation have been described in two previous reports that analyzed T. cruzi strains isolated from chagasic patients and from R. pallescens collected in central Panama [17], [28]. However, it must be kept in mind that molecular characterization of parasites isolated from autochthonous patients is necessary before confirming that TCI is the main lineage associated with the human T. cruzi infections found in this region.…”

Section: Discussionsupporting

confidence: 76%

A New Endemic Focus of Chagas Disease in the Northern Region of Veraguas Province, Western Half Panama, Central America

Saldaña

Pineda

Martinez³

et al. 2012

PLoS ONE

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BackgroundChagas disease was originally reported in Panama in 1931. Currently, the best knowledge of this zoonosis is restricted to studies done in historically endemic regions. However, little is known about the distribution and epidemiology of Chagas disease in other rural areas of the country.Methods and FindingsA cross-sectional descriptive study was carried out between May 2005 – July 2008 in four rural communities of the Santa Fe District, Veraguas Province. The study included an entomologic search to collect triatomines, bloodmeal type identification and infection rate with trypanosomes in collected vectors using a dot- blot and PCR analysis, genotyping of circulating Trypanosoma cruzi (mini-exon gene PCR analysis) and the detection of chagasic antibodies among inhabitants. The vector Rhodnius pallescens was more frequently found in La Culaca and El Pantano communities (788 specimens), where it was a sporadic household visitor. These triatomines presented darker coloration and larger sizescompared with typical specimens collected in Central Panama. Triatoma dimidiata was more common in Sabaneta de El Macho (162 specimens). In one small sub-region (El Macho), 60% of the houses were colonized by this vector. Of the examined R. pallescens, 54.7.0% (88/161) had fed on Didelphis marsupialis, and 24.6% (34/138) of T. dimidiata specimens collected inside houses were positive for human blood. R. pallescens presented an infection index with T. cruzi of 17.7% (24/136), with T. rangeli of 12.5% (17/136) and 50.7% (69/136) were mixed infections. In 117 T. dimidiata domestic specimens the infection index with T. cruzi was 21.4%. Lineage I of T. cruzi was confirmed circulating in these vectors. A T. cruzi infection seroprevalence of 2.3% (24/1,056) was found in this population.ConclusionsThis is the first report of Chagas disease endemicity in Santa Fe District, and it should be considered a neglected public health problem in this area of Panama.

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Section: Discussionsupporting

confidence: 76%

A New Endemic Focus of Chagas Disease in the Northern Region of Veraguas Province, Western Half Panama, Central America

Saldaña

Pineda

Martinez³

et al. 2012

PLoS ONE

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“…In Brazil and other countries in southern South America, TcI is less frequent in chronic chagasic patients, but most patients from Panama, Colombia, Ecuador, and Venezuela have been found to be infected with this DTU (Añez et al, 2004;Sousa et al, 2006;Herrera et al, 2007;Falla et al, 2009;Ocaña-Mayorga et al, 2010). Instances of patients infected with TcI who show severe cardiomyopathy have been reported from Colombia, Venezuela, and Paraíba state in northeastern Brazil, as well as from Mexico (Añez et al, 2004;Zafra et al, 2008;Ramírez et al, 2010), including severe clinical manifestations and even fatalities Sánchez-Guillén et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Trypanosoma cruzi I–III in southern Brazil causing individual and mixed infections in humans, sylvatic reservoirs and triatomines

et al. 2011

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The aim of this study was to characterise Discrete Typing Units (DTUs) of 28 isolates of Trypanosoma cruzi from humans (15), triatomines (9), and opossums (4) in the state of Paraná, southern Brazil. For this purpose, we analysed the size polymorphism at the 3' end of the 24Sα ribosomal RNA gene (rRNA) and the restriction fragment length polymorphism (RFLP) of the partial 5' sequence of the mitochondrial Cytochrome Oxidase subunit II gene (COII). Band patterns of the isolates were compared with reference samples of T. cruzi I (Silvio X10 and Col 17G2), T. cruzi II (Esmeraldo and JG), T. cruzi III (222 and 231), T. cruzi IV (CAN III), T. cruzi V (SO3 cl5), and T. cruzi VI (CL Brener). Our results confirmed that rRNA analysis is of limited use for assessing T. cruzi DTUs. COII RFLP analysis was suitable for screening, but for one isolate it was necessary to determine the COII partial sequence to identify the DTU. Only one of the isolates from humans belonged to T. cruzi I; 13 isolates belonged to T. cruzi II and one to T. cruzi III. The four isolates from opossums and five isolates from triatomines were identified as T. cruzi I. Four isolates from triatomines showed patterns of both T. cruzi I and II, indicating mixed infections. This study contributes to the characterisation of the dynamics of T. cruzi populations in southern Brazil.

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“…T. cruzi I, which has a very large geographical distribution from North to South America, predominates from the Amazonian basin northwards, where domestic and sylvatic triatomine species ensure the transmission of Chagas disease in various countries, including Venezuela, Colombia, Central America, and Mexico [9–13]. In the southern cone, countries such as Bolivia, Argentina, and Chile have reported the presence of T. cruzi I circulating in domestic and sylvatic transmission cycles [3, 14, 15].…”

Section: Introductionmentioning

confidence: 99%

Genetic Variability and Phylogenetic Relationships withinTrypanosoma cruziI Isolated in Colombia Based on Miniexon Gene Sequences

Herrera

Guhl

Falla

et al. 2009

Journal of Parasitology Research

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Phylogenetic studies of Trypanosoma cruzi have identified the existence of two groups: T. cruzi I and T. cruzi II. There are aspects that still remain unknown about the genetic variability within the T. cruzi I group. Given its epidemiological importance, it is necessary to have a better understanding of T. cruzi transmission cycles. Our purpose was to corroborate the existence of haplotypes within the T. cruzi I group and to describe the genetic variability and phylogenetic relationships, based on single nucleotide polymorphisms (SNPs) found in the miniexon gene intergenic region, for the isolates from different hosts and epidemiological transmission cycles in Colombian regions. 31 T. cruzi isolates were molecularly characterized. Phylogenetic relationships within T. cruzi I isolates showed four haplotype groups (Ia–Id), associated with their transmission cycle. In previous studies, we reported that haplotype Ia is mainly associated with the domestic cycle and domiciliated Rhodnius prolixus. Haplotype Ib is associated with the domestic cycle and peridomestic cycle, haplotype Ic is closely related with the peridomestic cycle, and haplotype Id is strongly associated with the sylvatic cycle. The phylogenetic methodologies applied in this study are tools that bolster the associations among isolates and thus shed light on Chagas disease epidemiology.

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Molecular characterization of human Trypanosoma cruzi isolates from endemic areas in Panama

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Cited by 23 publications

References 17 publications

A New Endemic Focus of Chagas Disease in the Northern Region of Veraguas Province, Western Half Panama, Central America

A New Endemic Focus of Chagas Disease in the Northern Region of Veraguas Province, Western Half Panama, Central America

Trypanosoma cruzi I–III in southern Brazil causing individual and mixed infections in humans, sylvatic reservoirs and triatomines

Genetic Variability and Phylogenetic Relationships withinTrypanosoma cruziI Isolated in Colombia Based on Miniexon Gene Sequences

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