Evaluation of adenosine deaminase seric activity in the diagnosis of bovine tuberculosis

Silva, Marcio Roberto; Mota, Pedro; Leite, Ricardo de Miranda Henriques; Lobato, Francisco Carlos Faria; Leite, Rômulo Cerqueira; Lage, Andrey Pereira

doi:10.1590/s0074-02762006000400008

Cited by 7 publications

(8 citation statements)

References 17 publications

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“…Missing data were not considered in the Chi-square test for these comparisons, in accordance with previous studies. 24 , l…”

Section: Methodsmentioning

confidence: 99%

“…Data on the TB status in Juiz de Fora are provided by the Municipal Secretariat of Health or found in scientific publications. 24 , 25 , 27 , f , h Furthermore, issues related to TB control remain unresolved: Does the treatment default rate in Juiz de Fora exceed the rate recommended by MS? Is the actual treatment default rate higher than the one reported to SINAN by the municipality?…”

Section: Introductionmentioning

confidence: 99%

“…Os dados da situação da TB em JF são os disponibilizados pela Secretaria Municipal de Saúde ou por publicações científicas. 24 , 25 , 27 , f , h Questões relacionadas ao controle da TB permanecem em aberto: A incidência de abandonos de tratamento em JF acontece acima do recomendado pelo Ministério da Saúde? A incidência real de abandonos é maior que a notificada pelo município ao SINAN A vigilância epidemiológica e a APS cumprem efetivamente seus papéis na detecção e notificação corretas dos encerramentos dos tratamentos?…”

Section: Introductionunclassified

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Profile and follow-up of patients with tuberculosis in a priority city in Brazil

Pereira

Silva

Costa³

et al. 2015

Rev. Saúde Pública

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OBJECTIVE To analyze the cases of tuberculosis and the impact of direct follow-up on the assessment of treatment outcomes.METHODS This open prospective cohort study evaluated 504 cases of tuberculosis reported in the Sistema de Informação de Agravos de Notificação (SINAN – Notifiable Diseases Information System) in Juiz de Fora, MG, Southeastern Brazil, between 2008 and 2009. The incidence of treatment outcomes was compared between a group of patients diagnosed with tuberculosis and directly followed up by monthly consultations during return visits (287) and a patient group for which the information was indirectly collected (217) through the city’s surveillance system. The Chi-square test was used to compare the percentages, with a significance level of 0.05. The relative risk (RR) was used to evaluate the differences in the incidence rate of each type of treatment outcome between the two groups.RESULTS Of the outcomes directly and indirectly evaluated, 18.5% and 3.2% corresponded to treatment default and 3.8% and 0.5% corresponded to treatment failure, respectively. The incidence of treatment default and failure was higher in the group with direct follow-up (p < 0.05) (RR = 5.72, 95%CI 2.65;12.34, and RR = 8.31, 95%CI 1.08;63.92, respectively).CONCLUSIONS A higher incidence of treatment default and failure was observed in the directly followed up group, and most of these cases were neglected by the disease reporting system. Therefore, effective measures are needed to improve the control of tuberculosis and data quality.

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“…Missing data were not considered in the Chi-square test for these comparisons, in accordance with previous studies. 24 , l…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionunclassified

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Profile and follow-up of patients with tuberculosis in a priority city in Brazil

Pereira

Silva

Costa³

et al. 2015

Rev. Saúde Pública

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“…Estimates of the sensitivity of tuberculin test range from 68-95% while specificity is estimated to be 96-99% (Monaghan et al 1994), and in the present study the sensitivity of SICTT was 24/30 (80%). Silva et al (2006) compared the seric levels of adenosine diaminase (ADA) to SICTT results during diagnosis of bovine TB, but the authors reported the impossibility to establish a meaningful cutoff for ADA seric values, concluding that the latter in its present form is of no use as an auxiliary test for diagnosis of bovine TB.…”

Section: Isolation and Identification Of Bovine Tuberculosis In A Bramentioning

confidence: 99%

Isolation and identification of bovine tuberculosis in a Brazilian herd (São Paulo)

Nassar

Miyashiro

Oliveira³

et al. 2007

Mem. Inst. Oswaldo Cruz

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Mycobacterium was verified in animals from a Brazilian dairy herd, a total of 42 samples from 30 cows were submitted to culture and the isolated strains were analyzed by two polymerase chain reaction (PCR), the first specific for species belonging to the Mycobacterium complex (MTBC) (Bouvet et al. 1993). From a clinical and epidemiological perspective, a rapid method to differentiate both species is important for timely diagnosis, effective treatment (due to the intrinsic resistance of M. bovis to pirazynamide) and epidemiology of TB (Shah et al. 2002).The bovine population in Brazil consists of about 180 million animals, with prevalence of TB ranging between 0.9 and 2.9% depending on the region and kind of production (Kantor & Ritacco 1994). Between 1989 and 1998, official notification data indicated a national prevalence of 1.3% of infected animals (Brasil 2005). Due to disgonic and very slow growth, the identification of M. bovis by conventional biochemical methods is cumbersome and timeconsuming. Polymerase chain reaction (PCR) is a sensitive and fast diagnostic tool that can be used to detect the agent in clinical samples in 48 h, but the presence of inhibitors in samples can interfere with its performance (Haddad et al. 2004, Singh et al. 2004, Brasil 2005.Among the several PCR systems developed for detection of species composing the M. tuberculosis complex (MTBC) (Wards et al. 1995, Brasil 2005, we focused on two. One was described by Niyaz Ahmed et al.(1999) and based on specific primers (NZ1 and NZ2) for the insertion sequence IS 1081, selectively present in organisms of the MTBC. A multiplex-PCR with primers JB and specific primers for the gene encoding for pyrazinimidase (pncA) was reported by Shah et al. (2002) to differentiate between M. bovis and M. tuberculosis on colonies in culture and this method could detect less than 20 pg of DNA, and this last reaction was the second applied reaction in the present work for differentiation between M. bovis and M. tuberculosis.We here present our work aiming at the detection of M. bovis mainly in retrofaryngeal lymphnode samples of bovines from a herd of Tatuí (São Paulo, Brazil) by microbiological culture, and MTBC and M. bovis specific PCR systems, and comparing the microbiological results with the positive or inconclusive results at single intradermal comparative tuberculin test (SICTT) presented by these animals.Thirty bovines from a Tatuí herd in São Paulo, Southeast Brazil and with positive (> 4 mm) or inconclusive (2 to 3.9 mm) response to SICTT were taken to sanitary slaughter. We collected 42 samples composed of the retrofaryngeal lymphnodes independent of the presence or not of suggestive lesions for TB while this was perfomed for prescapular lylmphnodes, lung, and liver only when presenting characteristic lesions of TB (Table). Samples were maintained in sterile bags at 4ºC for transport to the Laboratory of Tuberculosis of Instituto Biológico, São Paulo, Brazil until futher analysis.For culturing, samples were decontaminated by means of the Pet...

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“…Its activity has been reported in several diseases, including AIDS in humans, and is even considered to be an auxiliary diagnostic tool in some diseases such as tuberculosis [4,7]. In animals, total ADA activity is elevated in bovine leukosis [31], liver diseases [1], tuberculosis [26] and canine liver disease [3].…”

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confidence: 99%

Adenosine Deaminase Activity in Cats Infected with Feline Immunodeficiency Virus

Hankanga

Kobayashi

Yamada

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. Adenosine deaminase (ADA), an enzyme involved in purine metabolism, has been shown to be of clinical importance in several diseases in humans. To investigate whether ADA is of any clinical significance in cats, plasma adenosine deaminase (P-ADA) and T cell adenosine deaminase (T-ADA) activities were measured in feline immunodeficiency virus (FIV) negative and positive cats. The AIDS-related complex (ARC) group showed a significant elevation in P-ADA activity compared to the asymptomatic carrier (AC), and FIV-negative groups (P<0.005). T-ADA activity was significantly elevated in FIV-positive cats compared to the FIV-negative group (P<0.05) and this elevation was attributed to the increase in the ARC group (P<0.01). A correlation was found between P-ADA and T-ADA activities in the FIV-negative group. T-ADA activity and CD4 + cell number showed a strong negative correlation in FIV-positive cats (P<0.0005). CD4 + cell numbers were significantly reduced in the ARC group compared to the healthy controls (P<0.005). Our results showed that T-ADA is increased in FIV-positive cats during the ARC stage. These results also suggest that ADA may be an indicator of T cell activation in the ARC stage of FIV infection.

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Evaluation of adenosine deaminase seric activity in the diagnosis of bovine tuberculosis

Abstract: Determination of seric levels of adenosine deaminase (ADA), an enzyme produced by monocytes/macrophages and lymphocytes, has been used in the diagnosis of human tuberculosis (TB

Cited by 7 publications

References 17 publications

Profile and follow-up of patients with tuberculosis in a priority city in Brazil

Profile and follow-up of patients with tuberculosis in a priority city in Brazil

Isolation and identification of bovine tuberculosis in a Brazilian herd (São Paulo)

Adenosine Deaminase Activity in Cats Infected with Feline Immunodeficiency Virus

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