Superoxide dismutase in Cryptococcus neoformans varieties gattii, grubi, and neoformans

Dias, Amanda Latércia Tranches; Brigagão, Maı́sa Ribeiro Pereira Lima; Colepicolo, Pio; Siqueira, Antonio Martins de; Silva, E. G. Da; Paula, Claudete Rodrigues

doi:10.1590/s0074-02762006000100021

Cited by 7 publications

(3 citation statements)

References 16 publications

(15 reference statements)

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“…Given that different strains had different antioxidant capacity, mainly reflected in the activities of antioxidant enzymes, we suggested that the T. asahii clinical group stains may have higher antioxidant capacity than the environment group strains. According to previous studies, we also obtained that the mean SOD activity of Clinical group strains of T. asahii (10.45 ± 3.87 U/mg) was equivalent to that of Cryptococcus (12 ± 0.5 U/mg) [16], and below that of Candida dubliniensis (27.87 ± 20.82 U/mg) [15], significantly lower than that of C. albicans (151.8 ± 73.27 U/mg) [15]. The mean catalase activity of T. asahi Clinical group strains (48.51 ± 7.60 U/ mg) was significantly higher than that of A. fumigatus (1.75 ± 0.75 U/mg), Aspergillus terreus (3.5 ± 0.8 U/mg) [17].…”

Section: Discussionsupporting

confidence: 60%

Study on Antioxidant Enzymatic Activities of Trichosporon asahii

Zhang

Wang

2016

Indian J Microbiol

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Superoxide dismutase (SOD) and catalase are considered the most important antioxidant enzymes which protect fungus from the oxidant damage of reactive oxygen species. In this study, we collected 44 strains of () from different sources and investigated their SOD and catalase activities. The results showed that the SOD and catalase activities of Clinical group were significantly higher than those of Environment group ( < 0.01). The SOD and catalase activities of in Internal passage group went up gradually after passage in mice, and were significantly higher in 5th generation of Internal passage group ( < 0.05). The SOD and catalase activities of Fluconazole-resistant group strains also increased after resistant induction, and the SOD and catalase activities were significantly higher in the 10th generation of Fluconazole-resistant group ( < 0.05). This implied that has stronger antioxidant ability. The strains of from different sources have different antioxidant abilities, which mainly manifest in the difference of antioxidant enzymatic activities. Clinical group strains have the strongest antioxidant capacity; Internal passage group strains and Fluconazole resistant group strains better; Environmental group strains the lowest. These results also suggested that the antioxidant defensive response of might be relevant to its infection mechanism and drug resistance mechanism.

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Section: Discussionsupporting

confidence: 60%

Study on Antioxidant Enzymatic Activities of Trichosporon asahii

Zhang

Wang

2016

Indian J Microbiol

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“…As a response to zinc deprivation, homologous genes for the transcription factor Zap1 (Eide, 2009;Zhao & Eide, 1997) lead to the process of filamentation in Candida albicans and Aspergillus fumigatus, and have been considered as virulence factors and promising candidates for elucidating the mechanisms of pathogenicity (Bignell et al, 2005;Krappmann et al, 2004;Moreno et al, 2007). In this sense, the presence in our study of a low-affinity zinc ion transporter may indicate, indirectly, the need to eliminate reactive oxygen species through proteins such as cytosolic superoxide dismutase, a copper/zinc-dependent enzyme important for cell detoxification during oxidative metabolism, which is also regarded as a potential virulence factor (Cox et al, 2003;Dias et al, 2006;Narasipura et al, 2003Narasipura et al, , 2005.…”

Section: Discussionmentioning

confidence: 82%

Differential gene expression analysis of Paracoccidioides brasiliensis during keratinocyte infection

Silva

Matsumoto

Braz

et al. 2011

Journal of Medical Microbiology

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Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, one of the most important systemic fungal diseases in Latin America. This initiates in lung tissue and can subsequently disseminate to other tissues. Clinical manifestations range from localized forms to disseminated disease that can progress to lethality, probably depending on the relationships among the virulence of the fungus, the immune response and the ability to interact with the surface structures and invade epithelial cells and mononuclear cells of the host. It is generally regarded as a multifocal disease, with oral lesions as the prominent feature. The aim of this study was to evaluate P. brasiliensis yeast infection in normal oral keratinocytes (NOKs). The differential expression of mRNAs and proteins was also determined when the fungus was placed in contact with the cell in order to characterize differentially expressed genes and proteins during P. brasiliensis infection. After contact with NOKs, the fungus appeared to induce alterations in the cells, which showed cellular extensions and cavitations, probably resulting from changes in the actin cytoskeleton seen at 5 and 8 h after infection. Levels of protein expression were higher after reisolation of the fungus from infected NOK culture compared with culture of the fungus in medium. The analysis identified transcripts related to 19 proteins involved in different biological processes. Transcripts were found with multiple functions including induction of cytokines, protein metabolism, alternative carbon metabolism, zinc transport and the stress response during contact with NOKs. The proteins found suggested that the yeast was in a stress situation, as indicated by the presence of RDS1. Nevertheless, the yeast seemed to be proliferating and metabolically active, as shown by the presence of a proteasome, short-chain acetylator, glucosamine-6-phosphate isomerase and ADP/ATP carrier transcripts. Additionally, metabolic pathways may have been activated in order to eliminate toxic substances from the cell as a zinc transporter was detected, which is a potential target for the development of future drugs. INTRODUCTIONParacoccidioides brasiliensis is a thermodimorphic pathogenic fungus, the aetiological agent of paracoccidioidomycosis (PCM), an endemic disease geographically limited to Latin America and one of the most prevalent human deep systemic mycoses found in Brazil, Colombia and Venezuela (San-Blas et al., 2002). The transition from mycelium at 25 u C to yeast at 37 u C is essential for P. brasiliensis to establish the disease (Franco et al., 1997). P. brasiliensis may be inhaled and, once in the lungs, the fungus initiates a process of morphological transition into the pathogenic yeast form (San-Blas et al., 2002).PCM has a multiplicity of clinical presentations, from cutaneous to systemic forms, and can attack various tissues, especially the lungs (Benard & Franco, 2007). Oral mucosal lesions are commonly multiple, with a granular, mulberry-like surface and microulcerations, and ...

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“…Many of these factors are cuproproteins, and copper serves as the critical determinant for their functions. Identified virulence-associated cuproproteins include superoxide dismutase (SOD), laccase, copper-responsive transcriptional regulators, copper detoxification mechanisms, and copper chaperones [75,78,80,82,100,101,102].…”

Section: Copper Homeostasis In Fungimentioning

confidence: 99%

The Role of Copper Homeostasis at the Host-Pathogen Axis: From Bacteria to Fungi

Ding

2019

IJMS

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Copper is an essential trace element participating in many vital biological processes, however it becomes a toxic agent when in excess. Thus, precise and tight regulation of copper homeostasis processes, including transport, delivery, storage, detoxification, and efflux machineries, is important, ensuring that only the amount needed to sustain basic biological functions and simultaneously prevent copper toxicity in the cell is maintained. Numerous exciting studies have revealed that copper plays an indispensable role at the microbial pathogen-host axis for entities ranging from pathogenic bacteria to deadly fungal species. Analyses of copper homeostases in bacteria and fungi extensively demonstrate that copper is utilized by the host immune system as an anti-microbial agent. The expression of copper efflux and detoxification from microbial pathogens is induced to counteract the host’s copper bombardment, which in turn disrupts these machineries, resulting in the attenuation of microbial survival in host tissue. We hereby review the latest work in copper homeostases in pathogenic bacteria and fungi and focus on the maintenance of a copper balance at the pathogen-host interaction axis.

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Superoxide dismutase in Cryptococcus neoformans varieties gattii, grubi, and neoformans

Cited by 7 publications

References 16 publications

Study on Antioxidant Enzymatic Activities of Trichosporon asahii

Study on Antioxidant Enzymatic Activities of Trichosporon asahii

Differential gene expression analysis of Paracoccidioides brasiliensis during keratinocyte infection

The Role of Copper Homeostasis at the Host-Pathogen Axis: From Bacteria to Fungi

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