Efficacy of an enzyme-linked immunosorbent assay as a diagnostic tool for schistosomiasis mansoni in individuals with low worm burden

Oliveira, Edward; Kanamura, Hermínia Yohko; Lima, Danielle Malta

doi:10.1590/s0074-02762005000400013

Cited by 24 publications

(20 citation statements)

References 12 publications

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“…However, intrinsic problems in schistosomiasis immunodiagnosis, such as the difficulty in accurately differentiating between active infection, past infection, and reinfection, remain unresolved. Also, there are inherent specificity problems due to antibody cross-reactivity with different species of schistosomes, including reactivity with cercarial antigens from animal schistosomes, and with other helminth parasites (89)(90)(91).…”

Section: Immunological Diagnosismentioning

confidence: 99%

Advances in the Diagnosis of Human Schistosomiasis

et al. 2015

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SUMMARY Schistosomiasis is a major neglected tropical disease that afflicts more than 240 million people, including many children and young adults, in the tropics and subtropics. The disease is characterized by chronic infections with significant residual morbidity and is of considerable public health importance, with substantial socioeconomic impacts on impoverished communities. Morbidity reduction and eventual elimination through integrated intervention measures are the focuses of current schistosomiasis control programs. Precise diagnosis of schistosome infections, in both mammalian and snail intermediate hosts, will play a pivotal role in achieving these goals. Nevertheless, despite extensive efforts over several decades, the search for sensitive and specific diagnostics for schistosomiasis is ongoing. Here we review the area, paying attention to earlier approaches but emphasizing recent developments in the search for new diagnostics for schistosomiasis with practical applications in the research laboratory, the clinic, and the field. Careful and rigorous validation of these assays and their cost-effectiveness will be needed, however, prior to their adoption in support of policy decisions for national public health programs aimed at the control and elimination of schistosomiasis.

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Section: Immunological Diagnosismentioning

confidence: 99%

Advances in the Diagnosis of Human Schistosomiasis

et al. 2015

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“…4,13 In Brazil, several techniques have been tested in LEAs, such as São Paulo. 12,15,36,37 In Barra Mansa, as a result of implementation of a special program for controlling schistosomiasis (Programa Especial de Controle da Esquistossomose) in 1976, schistosomiasis prevalence was around 1%. 20 The aim of this study was to compare and evaluate the accuracy of the COPT, IFA-IgM, and parasitological diagnostic techniques in the identification of S. mansoni infections, by means of epidemiological inquiry of randomized fecal and serum samples from individuals living in areas peripheral to Barra Mansa, Rio de Janeiro.…”

Section: Discussionmentioning

confidence: 99%

“…48,50,51 Cross-reactivity with cercariae antigens from organisms that infect other species of animals are possible, 29 as are cross-reaction with other parasites. 15,36,52 53,54 and a decrease in sensitivity in infections with low parasite load. 35,36 The Kato and Miura technique, 25 modified by Katz and others 7 has become the international reference standard by the WHO.…”

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confidence: 99%

Detection of Schistosoma mansoni Antibodies in a Low-Endemicity Area Using Indirect Immunofluorescence and Circumoval Precipitin Test

Espírito-Santo

Pintó

Gargioni

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. Parasitological diagnostic methods for schistosomiasis lack sensitivity, especially in regions of low endemicity. The objective of this study was to determine the prevalence of Schistosoma mansoni infections by antibody detection using the indirect immunofluorescence assay (IFA-IgM) and circumoval precipitin test (COPT). Serum samples of 572 individuals were randomly selected. The IFA-IgM and COPT were used to detect anti-S. mansoni antibodies. Of the patients studied, 15.9% (N = 91) were IFA-IgM positive and 5.1% (N = 29) had COPT reactions (P 0.001 by McNemar's test). Immunodiagnostic techniques showed higher infection prevalence than had been previously estimated. This study suggests that combined use of these diagnostic tools could be useful for the diagnosis of schistosomiasis in epidemiological studies in areas of low endemicity.

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“…Nevertheless, there is a possibility to increase the sensitivity of Kato-Katz smear through examination of multiple samples [5] , but this is a limiting procedure for field study [7] . Moreover, this microscopic method is unsufficient in the areas of low endemicity, in post-treatment situations, and in the control of transmission [8,9] . Therefore, other diagnostic assays like detection of parasite-specific antibodies have been shown to be more sensitive than the parasitological examination and are needed to plan and monitor control measure [3,9] .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of microscopical and serological techniques in the diagnosis of Schistosoma mansoni infection at Sennar State, Central Sudan

Ibrahim

2014

Asian Pacific Journal of Tropical Disease

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Efficacy of an enzyme-linked immunosorbent assay as a diagnostic tool for schistosomiasis mansoni in individuals with low worm burden

Abstract: IgM-ELISA is an immunoenzymatic method useful for detection of IgM antibodies against a fraction of

Cited by 24 publications

References 12 publications

Advances in the Diagnosis of Human Schistosomiasis

Advances in the Diagnosis of Human Schistosomiasis

Detection of Schistosoma mansoni Antibodies in a Low-Endemicity Area Using Indirect Immunofluorescence and Circumoval Precipitin Test

Evaluation of microscopical and serological techniques in the diagnosis of Schistosoma mansoni infection at Sennar State, Central Sudan

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