Dried blood spots as a practical and inexpensive source for human immunodeficiency virus and hepatitis C virus surveillance

Toledo, Antonio; Januário, José Nélio; Rezende, Renata Maria Silva; Siqueira, Arminda Lúcia; Mello, Bernardo Freire de; Fialho, Érica Ligorio; Ribeiro, Raquel Andrade; Silva, Hélia Lemos da; Pires, E. C. F.; Simões, Taynãna César; Greco, Dirceu Bartolomeu

doi:10.1590/s0074-02762005000400005

Cited by 7 publications

(5 citation statements)

References 25 publications

(12 reference statements)

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“…In these regions, blood samples can be collected using DBS and transported to laboratories without refrigeration allowing more precise analysis in a shortened period of time. Finally, few studies have optimized commercial ELISA for anti‐HCV detection among DBS samples in Brazil [Toledo et al, 2005; Costa et al, 2009], and therefore, the present study may be useful for future researchers in this area.…”

Section: Discussionmentioning

confidence: 95%

“…Viral hepatitis markers have been detected from DBS samples [Tappin et al, 1998; Hutchinson et al, 2004; Costa et al, 2009; Tuaillon et al, 2010; Melgaço et al, 2011; Villar et al, 2011], but anti‐HCV detection from DBS samples exhibited different sensitivities, ranging from 87.5% to 100% [Parker et al, 1997, 1999; Abe and Konomi, 1998; McCarron et al, 1999; Montebugnoli et al, 1999; Solmone et al, 2002; Judd et al, 2003; Mendy et al, 2005; Toledo et al, 2005; Croom et al, 2006; Mahfoud et al, 2010].…”

Section: Introductionmentioning

confidence: 99%

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Dried blood spot samples: Optimization of commercial EIAs for hepatitis C antibody detection and stability under different storage conditions

Marques

Brandão

Silva

et al. 2012

Journal of Medical Virology

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This study was undertaken to optimize and compare the efficiency of two commercial EIAs for anti-HCV detection (HCV Ab Radim, Pomezzia, Italy and ETI-AB-HCVK-4 DiaSorin, Vercelli, Italy), in dried blood spot (DBS) samples. The long-term stability of anti-HCV on DBS samples stored at three environmental conditions was also evaluated at: 2-8 °C, 20-25 °C, and -20 °C. Paired DBS and serum samples were obtained from individuals with or without anti-HCV. The type of elution buffer, sample and conjugate volume, sample incubation time and cut-off values were evaluated. For both EIAs, a larger sample volume was used, and the cut-off value determined by the manufacturer was employed for Radim EIA; however, ROC curve analysis was used for the DiaSorin EIA. The sensitivity and specificity of Radim EIA on DBS were 97.5% and 99.5%, respectively, and of DiaSorin EIA were 88.9% and 98.9%, respectively. Accurate results were obtained for a period of 117 days using DBS samples stored at all storage conditions, but storage at -20 °C resulted in the lowest variation among the absorbance values. Both EIAs demonstrated the same limit of detection (until dilution of 1:10(4) with estimated viral load of 3.1 × 10(-1) UI/ml), but the Radim EIA was associated with the best performance because a low coefficient of variation was observed in the repetition and reproducibility studies. In conclusion, commercial EIAs can be optimized for anti-HCV detection in DBS samples that are extremely stable at different conditions for more than 100 days.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Dried blood spot samples: Optimization of commercial EIAs for hepatitis C antibody detection and stability under different storage conditions

Marques

Brandão

Silva

et al. 2012

Journal of Medical Virology

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“…The disruption of the virus envelope during the DBS drying process ensures biosafety, whereas well preserved antibodies and nucleic acids enable the detection of various infectious diseases (89), for example: measles (90), rubella (91) or even Ebola (92). DBS samples could also help with the actual summary of epidemiological situations concerning asymptomatic or long-incubation period diseases (for example, AIDS or chronic hepatitis, via HIV or HCV monitoring) (93).…”

Section: Applications Of the Dbs Samplesmentioning

confidence: 99%

The dried blood spot sampling method in the laboratory medicine

Andrlová¹,

Kanďár²

2019

BLL

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The minimization is currently expanding in all fi elds of human life. The smaller size of analytical instruments and their higher sensitivity enables an analysis of small amounts of a biological material with a very low consumption of chemicals, what is economically and also environmentally benefi cial. A patient friendly and minimal invasive sample collection is therefore more than required. The dried blood spot (DBS) sampling standardly used in the newborn screening (NS) may be an option. The sample collection is simple, non-invasive, does not require trained medical personnel assistance and also storing and transportation of these samples is much easier in comparison with the whole blood samples. The DBS sampling method is used in the therapeutic drug monitoring or in the diagnosis of infectious diseases and its usage is still spreading. In some cases, it is still only a research object, but it shows a big potential for a future use. It could completely replace the whole venous blood collection in some cases, for example in the metabolic screening of diabetic patients or monitoring of treatment response, and it can overall simplify the sample collection and the transportation process (Tab. 1, Ref. 152).

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“…The use of DBS as a diagnostic tool dates back to the 19th century, pioneered by Robert Guthrie for neonatal metabolic disorder screening [5]. In addition to screening for metabolic disorders, DBS cards have been utilised for human immunodeficiency virus (HIV) screening, laboratory quality control, drug testing and detection of pathogens in diverse sample types, including blood and dried plasma spots [6,7]. Numerous studies have also demonstrated that antibodies can be detected on DBS, such as in the prospective cohort study of congenital cytomegalovirus [8] and HIV infection [9].…”

Section: Introductionmentioning

confidence: 99%

Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review

et al. 2021

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Background Increasingly, vaccine efficacy studies are being recommended in low-and-middle-income countries (LMIC), yet often facilities are unavailable to take and store infant blood samples correctly. Dried blood spots (DBS), are useful for collecting blood from infants for diagnostic purposes, especially in low-income settings, as the amount of blood required is miniscule and no refrigeration is required. Little is known about their utility for antibody studies in children. This systematic review aims to investigate the correlation of antibody concentrations against infectious diseases in DBS in comparison to serum or plasma samples that might inform their use in vaccine clinical trials. Methods and findings We searched MEDLINE, Embase and the Cochrane library for relevant studies between January 1990 to October 2020 with no language restriction, using PRISMA guidelines, investigating the correlation between antibody concentrations in DBS and serum or plasma samples, and the effect of storage temperature on DBS diagnostic performance. We included 40 studies in this systematic review. The antibody concentration in DBS and serum/plasma samples reported a good pooled correlation, (r2 = 0.86 (ranged 0.43 to 1.00)). Ten studies described a decline of antibody after 28 days at room temperature compared to optimal storage at -20°C, where antibodies were stable for up to 200 days. There were only five studies of anti-bacterial antibodies. Conclusions There is a good correlation between antibody concentrations in DBS and serum/plasma samples, supporting the wider use of DBS in vaccine and sero-epidemiological studies, but there is limited data on anti-bacterial antibodies. The correct storage of DBS is critical and may be a consideration for longer term storage.

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Dried blood spots as a practical and inexpensive source for human immunodeficiency virus and hepatitis C virus surveillance

Cited by 7 publications

References 25 publications

Dried blood spot samples: Optimization of commercial EIAs for hepatitis C antibody detection and stability under different storage conditions

Dried blood spot samples: Optimization of commercial EIAs for hepatitis C antibody detection and stability under different storage conditions

The dried blood spot sampling method in the laboratory medicine

Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review

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