Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques

Abstract

Transmission of

Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil

“…According to WHO (1993), infection with Mycobacterium bovis is responsible for about 5% of human tuberculosis (Tb) cases in Brazil, suggesting the importance of better control of transmission from cattle to man (Parreiras et al 2004). In Brazil, the prevalence of the disease in bovines was estimated at 1.…”

Multiple drug-susceptibility screening in Mycobacterium bovis: new nucleotide polymorphisms in the embB gene among ethambutol susceptible strains

“…This is in agreement with previous studies where no M. bovis from animal origin were resistant to any of the antibiotics in question. 33,41 The mauve colour change observed at the EMB-resistance cutoff value suggests that a slight growth of mycobacteria did occur in some of the strains, a result that the d-REMA test was able to reveal. It is possible that this ambiguous chromatic change is a sign of borderline susceptibility as EMB is also a difficult drug to test with a poor agreement even among supranational reference laboratories.…”

“…While mycobacteria isolated from human cases are routinely assessed for drug susceptibility, studies regarding the resistance assessment of M. bovis isolated from animals to antibiotics used to treat human tuberculosis are limited. 32,33 M. bovis may spread to humans through the respiratory route and through the consumption of contaminated meat and dairy products. 2 Farmers and veterinarians are at a higher risk of infection.…”

Drug Susceptibility Testing of Mycobacteria Isolated from Humans and Cattle from Selected Sites of Ethiopia

“…Although the endemic nature of bovine tuberculosis (BTB) and the risk of human infection have been known for long, the pattern and extent of drug susceptibility of the etiologic agent of this human threat largely remained undetermined. Therefore, evaluation of drug susceptibility of M. bovis strains is important, not only for estimating such risk but also to understand the effect of uncontrolled use of antibiotics and the magnitude of influence of the transmission of drug resistant or MDR M. bovis strains on treatment of human TB [20], necessitating continued periodic monitoring of M. bovis isolates for development of antimicrobial resistance [16] [17].…”