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Cited by 48 publications
(49 citation statements)
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“…These include RT-PCR for hantavirus genome detection in clinical samples and ELISA using N recombinant protein from ARAV for use as the antigen in serological diagnostic tests for such infections [6][7][8] . These diagnostic techniques have been useful for diagnosing HPCS among patients in this region 13 .…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…These include RT-PCR for hantavirus genome detection in clinical samples and ELISA using N recombinant protein from ARAV for use as the antigen in serological diagnostic tests for such infections [6][7][8] . These diagnostic techniques have been useful for diagnosing HPCS among patients in this region 13 .…”
Section: Discussionmentioning
confidence: 99%
“…RT-PCR for genome amplification of S and M fragments of viral RNA segments was performed as previously described 8 . PCR amplicons were identified by size following electrophoresis on agarose gel, and were purified using the QIAquick kit (Qiagen, USA).…”
Section: Human Samplesmentioning
confidence: 99%
“…Campos et al, found maximum antibody titers of 1:400 9 . Seropositive samples were also tested by an RT-PCR that amplifies part of the small and the medium segments of hantavirus RNA, as previously reported, showing negative results 10 . Data from studied patients and test results are shown in Table 1.…”
mentioning
confidence: 99%
“…O diagnóstico laboratorial da infecção aguda por hantavírus pode ser feito por método sorológico, sendo muito utilizado no Brasil o ELISA com detecção de IgM e IgG contra antígenos de vírus Sin Nombre ou Los Andes 8 e por RT-PCR, amplificando os genes das proteínas N e G1, diretamente do sangue ou fragmento de órgão dos pacientes 20 . Nos casos fatais, a reação de imunohistoquímica em tecidos costuma ser utilizada para confirmar o diagnóstico.…”
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