2003
DOI: 10.1590/s0074-02762003000700010
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Abstract: Ultrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive reaction in amylopectin granules in bradyzoites, as well as in the wall and matrix of the cysts, with excellent increment of the ultrastructural morphology. Thi… Show more

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Cited by 17 publications
(8 citation statements)
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References 7 publications
(6 reference statements)
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“…Tissue-derived cysts from T. gondii, ME-49 strain, were obtained from the brains of previously infected C57BL/6 mice, according to the methods of Guimarães et al (2003), and maintained by serial intraperitonial passage in C57BL/6 mice.…”
Section: Parasitesmentioning
confidence: 99%
“…Tissue-derived cysts from T. gondii, ME-49 strain, were obtained from the brains of previously infected C57BL/6 mice, according to the methods of Guimarães et al (2003), and maintained by serial intraperitonial passage in C57BL/6 mice.…”
Section: Parasitesmentioning
confidence: 99%
“…The energy metabolism of bradyzoites is different from tachyzoites, and the most typical feature is massive amylopectin accumulated in the cytoplasm (Guimaraes et al ., 2003). T. gondii can store glucose in the form of amylopectin that can be found in several life stages (Guimaraes et al ., 2003; Guerardel et al ., 2005). It has been hypothesized that amylopectin granules may be an energy reserve for the conversion of bradyzoites to tachyzoites (Coppin et al ., 2003).…”
Section: Introductionmentioning
confidence: 99%
“…A critical question in the lifelong persistence of host Toxoplasma cysts is how these intracellular parasites are able to survive for long periods within the cyst. The nutrients required by bradyzoites surrounded by the cyst wall may be necessary for its maintenance, multiplication, and amylopectin granule synthesis [23,24]. The cyst wall, due to its limiting, highly invaginated membrane, provides an enhancement of the cyst surface area, affording the traffic of material between the parasite intracyst and the host cell cytosol [25][26][27].…”
Section: Discussionmentioning
confidence: 99%
“…Mice infected with ME-49 strain T. gondii and maintained for 4-8 weeks in C57BL/6 were euthanized and the brain cysts isolated as described [23]. License CEUA LW 50/14 from Fundação Oswaldo Cruz Committee of Ethics for Use of Animals was authorized.…”
Section: Parasitesmentioning
confidence: 99%