Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method

Abstract

A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBRgreen I to detect telomerase activity in a range of 10 2 to 10 7 parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.

Characterization and in vitro differentiation potential of a new human embryonic stem cell line, ReliCell®hES1

“…(c) Telomerase assay: Telomerase activity was determined using a non‐radioisotopic gel‐based standard telomerase repeat amplification protocol (TRAP) (Gang et al, 2000; Rubiano and Wasserman, 2003) using TRAPeze telomerase detection kit (Chemicon, USA). Briefly, a two‐step PCR reaction was performed (94°C for 30 sec and 59°C for 30 sec) for 35 cycles with 1–6 μg of protein extracted from 50 to 70 hESC colonies.…”