Crossing experiments detect genetic incompatibility among populations of Triatoma brasiliensis Neiva, 1911 (Heteroptera, Reduviidae, Triatominae)

Abstract

Triatoma brasiliensis is composed of at least four geographic populations (brasiliensis, melanica, macromelasoma, and juazeiro)

Biological and genetic aspects of experimental hybrids from species of the Phyllosoma complex (Hemiptera: Reduviidae: Triatominae)

“…Another reason is the existence of a correlation between geographic distance and degree of reproductive isolation, since the species that have been collected parapatrically in various areas, such as M. longipennis and M. picturatus (Martínez-Ibarra et al 2001, 2008, Magallón-Gastélum 2006, M. pallidipennis and M. longipennis, and M. pallidipennis and M. picturatus, presented lower degrees of reproductive isolation (Martínez-Ibarra et al 2005). Similar results were reported when four morphologically different T. brasiliensis populations were studied; having lower degrees of reproductive isolation, those populations (brasiliensis and macromelanosoma) were collected from geographically close areas (Costa et al 2003). Likewise, the relationship between M. longipennis and M. mazzottii can not be explained, since they have rarely been collected parapatrically, though they are phylogenetically close.…”

“…Similarly, even when crosses between M. mazzottii and M. longipennis did produce a second generation, all F2 males were infertile, showing their degree of reproductive isolation. Those phenomena were similar to that described in Costa et al (2003), where four morphologically different populations of Triatoma brasiliensis Neiva (brasiliensis, melanica, macromelanosoma and juazeiro) were crossed in order to evaluate their genetic and reproductive compatibility. This paper recorded low compatibility levels between brasiliensis and melanica, which became even lower when a melanica female was in the cross, similar to our work where lower compatibility levels were recorded between M. mazzottii and M. picturatus, mainly when the female was from the M. picturatus species.…”

Phylogeny of Triatoma sherlocki (Hemiptera: Reduviidae: Triatominae) Inferred from Two Mitochondrial Genes Suggests Its Location Within the Triatoma brasiliensis Complex

“…2,4,14 Triatoma brasiliensis shows remarkable chromatic variation across its geographic distribution. 15 This characteristic led this "species" to be subjected to a multi-disciplinary study of its morphology, biology, ecology, isoenzymes, and mitochondrial DNA variation.…”

“…15 This characteristic led this "species" to be subjected to a multi-disciplinary study of its morphology, biology, ecology, isoenzymes, and mitochondrial DNA variation. 14,[16][17][18][19][20] It was concluded that T. brasiliensis is in fact a complex of species based on three allopatric evolutionary units: T. brasiliensis Neiva, 1911, T. brasiliensis melanica Neiva and Lent, 1941 and T. brasiliensis macromelasoma Galvão, 1956. 15 Molecular markers based on DNA have led to considerable progress in the understanding of genetic relatedness, phylogeny, and population dynamics in triatomines during the last 10 years.…”

Deposition, Incubation Period and Hatching of Eggs from Triatoma juazeirensis Costa & Felix and Triatoma sherlocki Papa, Jurberg, Carcavallo, Cerqueira & Barata, (Hemiptera: Reduviidae) under Laboratory Conditions

“…Recently, multidisciplinary studies suggest that of T. brasiliensis represent a complex species compounded by four members: Triatoma brasiliensis brasiliensis Neiva, Triatoma brasiliensis macromelasoma Galvão, Triatoma melanica Neiva & Lent, Triatoma juazeirensis Costa & Felix. In these studies, were analyzed the following data concerning four populations: 1 -morphological, demonstrating differences in the ornamentation on the exochorion of the eggs (Costa et al 1997a), 2 -isoenzymatic, where the analysis of nine loci by multilocus enzyme eletroforesis showed significant genetic distances (Costa et al 1997b), 3 -ecological, proving by ecological niche modeling that the staining patterns are stable and not influenced by the different environments in which the populations are distributed (Costa et al 2002 ) 4 -biological, detecting low reproductive compatibility in crossing experiments (Costa et al 2003b), 5 -genetic, by analyzing the mtDNA fragments of Cyt B, it was possible to detect genetic differentiation (monteiro et al 2004). Later, menDonça et al (2009) by carrying out the sequencing of the mtDNA fragments of Cyt B and 16S ribosomal RNA it was possible to place the Triatoma sherlocki Papa, Jurberg, Carcavallo, Cerqueira & Barata in T. brasiliensis complex.…”