Frequency of Infection of Lutzomyia Phlebotomines with Leishmania braziliensis in a Brazilian Endemic Area as Assessed by Pinpoint Capture and Polymerase Chain Reaction

Study on phlebotomine sand fly (Diptera: Psychodidae) fauna in Belo Horizonte, state of Minas Gerais, Brazil

“…Based on the results of the current work, the use of Shannon traps allowed us to evaluate the presence of some species that were not collected (Passos et al 1993) and it has been shown to be highly sensitive in detecting protozoa parasites into phlebotomine sand flies (Rodriguez et al 1999, Miranda et al 2002, Michalsky et al 2002. Nevertheless, previous reports have shown that phlebotomine infectivity rates in nature are low, 0.2 to 2%, making the parasite finding difficult (Rodriguez et al 1999, Miranda et al 2002.…”

Flebótomos de áreas com ocorrências de casos humanos de leishmaniose tegumentar americana no Município de Seropédica, Estado do Rio de Janeiro

“…Em uma área endêmica de LTA na Bahia, foi obtido um índice de 0,4 %, taxa suficiente para a manutenção da endemicidade da infecção 17 . A investigação da infecção em flebotomíneos capturados de forma não direcionada mostra uma taxa ainda mais baixa ou mesmo nula 27 .…”

Investigation of natural infection by Leishmania in sandflies of Paraná State, Southern Brazil

“…braziliensis (Lonardoni et al, 1993;Silveira et al, 1999). The PCR has been widely used in the study of the natural and experimental sandfly infection, using various primers (Pérez et al, 1994;Rodriguez et al, 1999;Aransay et al, 2000;Michalsky et al, 2002;Miranda et al, 2002;PitaPereira et al, 2005;Kato et al, 2005;Jorquera et al, 2005). According to Arevalo et al, (1993), the primers MP1L and MP3H, which amplify a fragment of 70 bp of kDNA from parasites of the Leishmania (Viannia) subgenus, as well as being very sensitive, have been successfully tested for the diagnosis and detection of sandflies naturally infected with promastigotes.…”