IgG Anti-IgE Autoantibodies in Visceral Leishmaniasis

Atta, Ajax Mercês; Sousa-Atta, M.L.B.; Júnior, Argemiro D’Oliveira; Almeida, Roque Pacheco de; Araújo, Maria Ilma; Carvalho, Edgar M.

doi:10.1590/s0074-02762002000100017

Cited by 7 publications

(7 citation statements)

References 16 publications

(16 reference statements)

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“…This study was the second to demonstrate the presence of an IgG autoantibody against IgE as the causative agent of ATR from blood components . IgG autoantibodies against IgE are found in patients with various diseases, such as allergic disorders, systemic lupus erythematosus, parasite infection, systemic sclerosis, Crohn's disease, and visceral leishmaniasis . Such autoantibodies are also detected in normal individuals, as reported in some studies .…”

Section: Discussionsupporting

confidence: 73%

“…14 39 Crohn's disease, 40 and visceral leishmaniasis. 41 Such autoantibodies are also detected in normal individuals, as reported in some studies. 32,40 Although the presence of such a pathogenetic auto anti-IgE antibody in blood components may be rare, it is important to keep in mind that the IgG autoantibody against IgE might be present in blood components, when the mechanisms of ATR are analyzed.…”

Section: Identification Of Causative Agent In the Ffp-atr1 By The Batsupporting

confidence: 67%

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Immunoglobulin (Ig)G antibodies against IgE identified by basophil activation test as the putative causative agent of a serious allergic transfusion reaction: potential utility of the test as a new safety measure for allergic transfusion reactions

et al. 2018

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BACKGROUND In most cases of allergic transfusion reactions (ATRs), the causative agents have not been identified and the mechanisms are largely unknown, with a few exceptions. The basophil activation test (BAT) was recently introduced in the field of transfusion to investigate the causal relationships between ATRs and transfusion, as well as the mechanisms behind them. STUDY DESIGN AND METHODS The BAT was used to screen the residual supernatants (SNs) of 43 blood components associated with serious ATRs for those that can activate basophils of many healthy volunteers. The SNs were then fractionated by centrifugal ultrafiltration and protein G column chromatography and each separated fraction was reexamined by the BAT. RESULTS Of the 43 such blood components, one activated basophils from 19 of 21 healthy volunteers. In the blood component, the IgG antibody against IgE was identified as a putative causative agent. CONCLUSION Blood donors who possessed the IgG antibody against IgE may be dangerous to transfusion recipients. The BAT would be useful in identifying such high‐risk blood donors, when it is used to screen the blood components associated with serious ATRs for residual SNs that can activate the basophils of many healthy volunteers.

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Section: Discussionsupporting

confidence: 73%

Section: Identification Of Causative Agent In the Ffp-atr1 By The Batsupporting

confidence: 67%

Immunoglobulin (Ig)G antibodies against IgE identified by basophil activation test as the putative causative agent of a serious allergic transfusion reaction: potential utility of the test as a new safety measure for allergic transfusion reactions

et al. 2018

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“…IgE immunocapture ELISA using polystyrene microplates containing wells coated with 500 ng of goat IgG anti-human IgE and a goat IgG anti-human IgG peroxidase conjugate from the same source was used to detect anti-IgE IgG autoantibodies (15). This assay detects IgG autoantibodies that recognize IgE epitopes, which are exposed after the capture of this immunoglobulin by goat IgG anti-human IgE adsorbed to the solid phase.…”

mentioning

confidence: 99%

Immunoglobulin E and systemic lupus erythematosus

Atta

Sousa

Carvalho

et al. 2004

Braz J Med Biol Res

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Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by intense polyclonal production of autoantibodies and circulating immune complexes. Some reports have associated SLE with a Th2 immune response and allergy. In the present study 21 female patients with SLE were investigated for total IgE and IgE antibodies to dust house aeroallergens by an automated enzymelinked fluorescent assay, and were also evaluated for antinuclear IgE autoantibodies by a modified indirect immunofluorescence test using HEp-2 cells as antigen substrate. Additionally, immunocapture ELISA was used to investigate serum anti-IgE IgG autoantibodies. Serum IgE above 150 IU/ml, ranging from 152 to 609 IU/ml (median = 394 IU IgE/ml), was observed in 7 of 21 SLE patients (33%), 5 of them presenting proteinuria, urinary cellular casts and augmented production of anti-dsDNA antibodies. While only 2 of 21 SLE patients (9.5%) were positive for IgE antibodies to aeroallergens, all 10 patients with respiratory allergy (100%) from the atopic control group (3 males and 7 females), had these immunoglobulins. SLE patients and healthy controls presented similar anti-IgE IgG autoantibody titers (X = 0.37 ± 0.20 and 0.34 ± 0.18, respectively), differing from atopic controls (0.94 ± 0.26). Antinuclear IgE autoantibodies were detected in 17 of 21 (81%) sera from SLE patients, predominating the fine speckled pattern of fluorescence, that was also observed in IgG-ANA. Concluding, SLE patients can present increased IgE levels and antinuclear IgE autoantibodies without specific clinical signs of allergy or production of antiallergen IgE antibodies, excluding a possible association between SLE and allergy.

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“…Immunocapture ELISA was used to detect anti-IgE IgG autoantibodies (14). Briefly, the polystyrene microwells of an ELISA plate (Maxisorp, Nunc, Roskilde, Denmark) were coated with 500 ng of anti-human IgE goat IgG antibodies (Sigma Chemical Co., St. Louis, MO, USA) and their reactive sites were blocked with 1% BSA in PBS.…”

mentioning

confidence: 99%

Autoantibody production in chronic idiopathic urticaria is not associated with Helicobacter pylori infection

Atta

Rodrigues

Sousa

et al. 2004

Braz J Med Biol Res

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Chronic idiopathic urticaria (CIU) is a dermatological syndrome, characterized by raised erythematous skin lesions, that affects 20% of the general population and has been associated with autoimmunity. However, some reports have also suggested a close relationship between CIU and Helicobacter pylori infection, which is endemic in developing countries and associated with chronic gastritis, peptic ulcer disease, and gastric carcinoma. In the present study, we investigated the occurrence of autoantibodies in sera from 23 CIU subjects infected with H. pylori and from 23 CIU subjects without this infection. The presence of anti-thyroid antibodies was determined by indirect hemagglutination assay and the presence of autoantibodies to IgE and C1INH was determined by ELISA. Antibodies to thyroid antigens were detected at low titers from 100 to 400 in three of 23 (13%) CIU-infected subjects and in four of 23 (17%) CIU-noninfected subjects. The titers of anti-IgE autoantibodies were similar in these CIU groups, presenting absorbances of 1.16 ± 0.09 and 1.07 ± 0.16, respectively, while a titer of 1.14 ± 0.15 was detected in the healthy control group. The concentration of anti-C1INH autoantibodies was the same in the CIU-infected and -noninfected subjects (7.28 ± 1.31 and 7.91 ± 2.45 ng/ml, respectively), and was 7.20 ± 2.25 ng/ml in the healthy control group. However, the serum levels of complexed anti-C1INH antibodies were increased in CIU-infected subjects compared to CIU-noninfected subjects and healthy controls with an absorbance of 1.51 ± 0.21 vs 1.36 ± 0.16 and 1.26 ± 0.23, respectively (P < 0.05), indicating an impaired clearance of immune complexes in CIUinfected patients. In conclusion, no correlation was observed between H. pylori infection and autoantibody production in CIU patients consistent with reports of clinical studies.

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IgG Anti-IgE Autoantibodies in Visceral Leishmaniasis

Abstract: Procedures for IgG depletion in visceral leishmaniasis (VL)

Cited by 7 publications

References 16 publications

Immunoglobulin (Ig)G antibodies against IgE identified by basophil activation test as the putative causative agent of a serious allergic transfusion reaction: potential utility of the test as a new safety measure for allergic transfusion reactions

Immunoglobulin (Ig)G antibodies against IgE identified by basophil activation test as the putative causative agent of a serious allergic transfusion reaction: potential utility of the test as a new safety measure for allergic transfusion reactions

Immunoglobulin E and systemic lupus erythematosus

Autoantibody production in chronic idiopathic urticaria is not associated with Helicobacter pylori infection

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