Comparison between precipitin and ELISA tests in the bloodmeal detection of Aedes aegypti (Linnaeus) and Aedes fluviatilis (Lutz) mosquitoes experimentally fed on feline, canine and human hosts

Gomes, Luciana Almeida Martins; Duarte, Rosemere; Lima, Dinair Couto; Diniz, Beatriz Sanson; Serrão, Maria Lúcia; Labarthe, Norma

doi:10.1590/s0074-02762001000500020

Cited by 34 publications

(21 citation statements)

References 8 publications

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“…Of the multiple-reactive mosquitoes, 64.7% reacted to bird antiserum (Table 1). The total proportion of mosquitoes feeding on birds (26.5%) was similar to proportions found in São Paulo state (Gomes et al 2001), as well as those recorded in the majority of observations by Molaei et al (2007). The only two observations by Molaei et al (2007) that were not similar were from Australia (Lee et al 1954;Kay et al 1985), where a very high proportion of bird feeding was recorded.…”

supporting

confidence: 79%

“…The technique is more sensitive than double immunodiffusion (Lorosa et al 1999), and although less sensitive, has better speciÞcity and accuracy in blood source analysis of Aedes aegypti (L.) and Aedes fluviatilis (Lutz) than the enzyme-linked immunosorbent assay (ELISA) (Gomes et al 2001). Forattini et al (1987) used this relatively simple and economical technique and considered the use of 13 antisera to be excessive due to the dilution of material.…”

mentioning

confidence: 99%

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Feeding Patterns ofCulex quinquefasciatus(Diptera: Culicidae) From Eastern Santa Catarina State, Brazil: Table 1.

Alencar¹,

Silva²,

Oliveira³

et al. 2012

J Med Entomol

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Blood-feeding sources of Culex quinquefasciatus Say (Diptera: Culicidae) collected in the eastern region of the Brazilian state of Santa Catarina were analyzed by precipitin technique. One hundred fifty-four female mosquitoes collected by CDC traps in the Navegantes municipality 13-15 February 2005 reacted to one or more of eight antisera, including chicken, dog, goat, sheep, horse, opossum, human and rodent antisera. One hundred thirty-seven specimens (89%) reacted to only one source, and 17 (11%) specimens reacted to two sources. Among the 137 specimens reacting to only one source, reactions to rodent (50.4%), sheep (5.8%), chicken (5.1%), goat (5.1%), dog (2.2%), horse (3.6%), and human (3.6%) antisera were observed. The analyzed species demonstrated a high degree of opportunistic feeding behavior in relation to host preference. Results are compared with results from similar studies, and the low proportion of reactions to human antisera is discussed.

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supporting

confidence: 79%

mentioning

confidence: 99%

Feeding Patterns ofCulex quinquefasciatus(Diptera: Culicidae) From Eastern Santa Catarina State, Brazil: Table 1.

Alencar¹,

Silva²,

Oliveira³

et al. 2012

J Med Entomol

View full text Add to dashboard Cite

show abstract

“…All such specimens eventually tested negative for antigens of potential hosts. This was the best option in the absence of unfed laboratory-reared flies which researchers on other insects have used as negative controls (Blackwell et al, 1994;Gomes et al, 2001;Ponlawat and Harrington, 2005). No success has been achieved with laboratoryrearing of Australian tabanids.…”

Section: Tabanid Sample Preparation For Elisamentioning

confidence: 99%

“…Plates were then incubated in the dark at room temperature for one hour after which Optical Density (OD) was measured at dual wavelengths 414 nm and 492 nm (Hall et al, 1987;Reid and Copeman, 2003) using an automatic ELISA plate-reader (Multiskan Ascent V1.24, Thermo Electron Corporation) interfaced to a computer with Ascent Software Version 2.6. The cut-off value for a negative result in all types of ELISA was calculated as the mean OD of negative controls plus two standard deviations (Beier et al, 1988;Gomes et al, 2001;Reid and Copeman, 2003). A sample was considered positive if ODs for duplicate wells were both above the negative cut-off value.…”

Section: Direct Elisa For Detection Of Cow Antigensmentioning

confidence: 99%

Host preferences of tabanid flies based on identification of blood meals by ELISA

Muzari

Burgess

Skerratt

et al. 2010

Veterinary Parasitology

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“…However, Ae. aegypti does feed on dogs when humans are less accessible, and cats in the laboratory (Gomes et al 2001, Suwonkerd et al 2006. Aedes albopictus is also anthropophilic, with 24% of blood meals derived from humans in North Carolina .…”

Section: Denvmentioning

confidence: 99%

Influence of Hosts on the Ecology of Arboviral Transmission: Potential Mechanisms Influencing Dengue, Murray Valley Encephalitis, and Ross River Virus in Australia

Carver

Bestall

Jardine

et al. 2009

Vector-Borne and Zoonotic Diseases

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Ecological interactions are fundamental to the transmission of infectious disease. Arboviruses are particularly elegant examples, where rich arrays of mechanisms influence transmission between vectors and hosts. Research on host contributions to the ecology of arboviral diseases has been undertaken within multiple subdisciplines, but significant gaps in knowledge remain and multidisciplinary approaches are needed. Through our multidisciplinary review of the literature we have identified five broad areas where hosts may influence the ecology of arboviral transmission: host immunity; cross-protective immunity and antibody-dependent enhancement; host abundance; host diversity; and pathogen spillover and dispersal. Herein we discuss the known and theoretical roles of hosts within these topics and then apply this knowledge to three epidemiologically important mosquitoborne arboviruses that occur in Australia: dengue virus (DENV), Murray Valley encephalitis virus (MVEV), and Ross River virus (RRV). We argue that the underlying mechanisms by which hosts influence arboviral activity are numerous and attempts to delineate these mechanisms further are needed. Investigations that focus on hosts of vector-borne diseases are likely to be rewarding, particularly where the ecology of vectors is relatively well understood. From an applied perspective, enhanced knowledge of host influences upon vector-borne disease transmission is likely to enable better management of disease burden. Finally, we suggest a framework that may be useful to identify and determine host contributions to the ecology of arboviruses.

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Comparison between precipitin and ELISA tests in the bloodmeal detection of Aedes aegypti (Linnaeus) and Aedes fluviatilis (Lutz) mosquitoes experimentally fed on feline, canine and human hosts

Abstract: The identification of arthropod bloodmeals is important in many epidemiological

Cited by 34 publications

References 8 publications

Feeding Patterns ofCulex quinquefasciatus(Diptera: Culicidae) From Eastern Santa Catarina State, Brazil: Table 1.

Feeding Patterns ofCulex quinquefasciatus(Diptera: Culicidae) From Eastern Santa Catarina State, Brazil: Table 1.

Host preferences of tabanid flies based on identification of blood meals by ELISA

Influence of Hosts on the Ecology of Arboviral Transmission: Potential Mechanisms Influencing Dengue, Murray Valley Encephalitis, and Ross River Virus in Australia

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