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Cited by 14 publications
(16 citation statements)
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References 6 publications
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“…The reactions were carried out in a thermal cycler (PxE0.2, Thermo Electron Corporation™, Milford, MA, USA) according to the following steps: 94ºC for 5min, 35 denaturation cycles at 94ºC, primer hybridization at 58ºC, extension at 72ºC, 72ºC for 10min, and 4ºC for 10min. All reactions were conducted in triplicate, and the DNA of a L. (L.) chagasi culture (Merivaldo strain, IOC-LC2455, isolated from a VL patient in an endemic area in Jequié) 17 was used as positive control. Finally, the amplicons were analyzed by horizontal electrophoresis on agarose gels 2% (m/v) stained with ethidium bromide 0.2μg/mL at 90V for 90min.…”
Section: Primer-specific Pcrmentioning
confidence: 99%
“…The reactions were carried out in a thermal cycler (PxE0.2, Thermo Electron Corporation™, Milford, MA, USA) according to the following steps: 94ºC for 5min, 35 denaturation cycles at 94ºC, primer hybridization at 58ºC, extension at 72ºC, 72ºC for 10min, and 4ºC for 10min. All reactions were conducted in triplicate, and the DNA of a L. (L.) chagasi culture (Merivaldo strain, IOC-LC2455, isolated from a VL patient in an endemic area in Jequié) 17 was used as positive control. Finally, the amplicons were analyzed by horizontal electrophoresis on agarose gels 2% (m/v) stained with ethidium bromide 0.2μg/mL at 90V for 90min.…”
Section: Primer-specific Pcrmentioning
confidence: 99%
“…Ultrapure water was used instead of the DNA sample in the negative control. The L. infantum DNA strain MHOM/BR2000/MER2 (FIOCRUZ/BA; Merivaldo strain, IOC-LC2455), which had been isolated from a VL patient in an endemic area of the town of Jequié, state of Bahia, Brazil, was used as the positive control (PARANHOS-SILVA et al, 2001). The reactions were carried out in a thermal cycler (PxE 0.2, Thermo Electron Corporation) with the following cycling parameters: 94 °C for 5 minutes; 35 cycles at 94 °C for 45 seconds, 58 °C for 45 seconds and 72 °C for 45 seconds; and an annealing cycle at 72 °C for 10 minutes and 4 °C for 10 minutes.…”
Section: Molecular Diagnosismentioning
confidence: 99%
“…Leishmania amazonensis (MHOM/BR/88/BA-125), Leila strain, was gently provided by Dr. Aldina Barral, Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil; L. braziliensis (MOM3456, isolated from a human case of cutaneous leshmaniosis) was a gift from Dr. Margarida Pompeu, Federal University of Ceará, Brazil and L. chagasi (MHOM/BR2000/Merivaldo2) was isolated from a patient with visceral leishmaniasis, from Jequie, Brazil (Paranhos-Silva et al, 2001). All the parasites were characterized by isoenzymatic profile and reactivity with monoclonal antibody by Dr. Gabriel Grimaldi, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro.…”
Section: Parasite Antigensmentioning
confidence: 99%
“…In the diagnostic routine, however, Leishmania species differing from those implicated in the genesis of disease are used as source of antigens (Paranhos-Silva et al, 2001;Zijlstra et al, 2001;EL-Safi et al, 2002;Gavgani et al, 2002;Borges et al, 2003). The reasons for such practice are (1) the wide cross-reactivity of the immune responses against different Leishmania species and (2) the costs inherent to antigen production and quality control of the antigen batches.…”
Section: Introductionmentioning
confidence: 99%