In vivo lymphocyte activation and apoptosis by lectins of the Diocleinae subtribe

Bessa, Theolis Costa Barbosa; Arruda, Sérgio; Cavada, Benildo Sousa; Grangeiro, Thalles Barbosa; Freitas, Luiz Antônio Rodrigues de; Netto, Manoel Barral

doi:10.1590/s0074-02762001000500016

Cited by 50 publications

(19 citation statements)

References 19 publications

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“…This difference can also be attributed to slight changes in key amino acid sequences in the carbohydrate-binding site involved with sugar interaction that may affect the fine specificity for sugar in the cell membrane as well as the ratio between divalent and tetravalent forms [11]. Moreover, lectins showed distinct pH-dependency in the formation of dimeric or tetrameric arrangements, which can result in different effects [31] because the tetravalent form is only able to cause cross-linking of receptors on the cell membrane, thereby triggering the activation of the signal transduction pathways [4].…”

Section: Discussionmentioning

confidence: 99%

“…This binding can change cell physiology as a result of cell agglutination [1,2]. In addition, lectins have shown strong mitogenic activity to lymphocytes [3,4] and have been used to differentiate malignant tumors from benign cells, and their degree of glycosylation is associated with metastasis [5]. Several studies have also demonstrated that lectins inhibit cell adhesion and proliferation and showed cytotoxic effects on human tumor cells [6].…”

Section: Introductionmentioning

confidence: 99%

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Influence of fetal bovine serum on cytotoxic and genotoxic effects of lectins in MCF‐7 cells

Faheina–Martins

Silveira

Ramos

et al. 2011

J Biochem & Molecular Tox

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Canavalia ensiformis (ConA), Canavalia brasiliensis (Conbr), and Cratylia floribunda (CFL) lectins have exhibited glucose-mannose binding specificity. We investigated the effect of fetal bovine serum (FBS) concentrations (1, 5, 10, and 20%) on the cytotoxic effect of these lectins against breast tumor cell line MCF-7. Cell viability was examined using the MTT reduction assay. When cells were grown in a medium supplemented with a higher serum concentration (10 or 20%), all lectins were much less toxic. When we used 1% FBS, it was possible to achieve a concentration-dependent activity by all examined lectins, with an IC(50) of 3.5, 25, and 60 µg/mL for ConA, Conbr, and CFL, respectively. All lectins incubated with 1% FBS induced apoptosis and DNA damage in MCF-7 cells. We conclude that ConA, Conbr, and CFL lectins' cytotoxic and genotoxic effects were observed only at low concentrations of serum.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of fetal bovine serum on cytotoxic and genotoxic effects of lectins in MCF‐7 cells

Faheina–Martins

Silveira

Ramos

et al. 2011

J Biochem & Molecular Tox

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“…This property has long been exploited to introduce lectins to detect, isolate and characterize oligosaccharide structures from unrelated biological sources [7,8]. Their carbohydrate-binding activity usually signals metabolic activation or interferes/modulates a specific cellular response in free-living cells or animal models [9,10]. Their critical ability to detect changes in oligosaccharide structures is a useful resource in studies where these changed oligosaccharide structures reflects metabolic alterations observed in malignant cells [11,12].…”

Section: Introductionmentioning

confidence: 99%

Embryotoxic activity and differential binding of plant-derived carbohydrate-recognizing proteins towards the sea urchin embryo cells

Macedo

Costa-Lotufo

Wilke

et al. 2005

International Journal of Biological Macromolecules

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“…[31]. Moreover, ConBr showed different biological effects than ConA with respect to peripheral biological activities [11], including induction of rat paw edema [51], peritoneal macrophage spreading in the mouse [52], in vivo human lymphocyte stimulation [53], induction of histamine release [54,55] and NO release [56]. Thus, it is possible that differences in the quaternary conformation of ConBr, as compared with ConA, may be the basis for these divergent phenotypes [30].…”

Section: Discussionmentioning

confidence: 99%

ConBr, a Lectin from Canavalia brasiliensis Seeds, Protects Against Quinolinic Acid-Induced Seizures in Mice

et al. 2011

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Lectins are proteins capable of reversible binding to carbohydrates or glycoconjugates. In the central nervous system of mammals, lectins with affinity for mannose/glucose or galactose can modulate cellular communication. ConBr, a lectin isolated from the seeds of Canavalia brasiliensis, previously showed antidepressant effect in the forced swimming test in mice, with involvement of the monoaminergic system. In this study, we investigated the neuroprotective effects of ConBr against quinolinic acid (QA), a well-known NMDA agonist that produces severe neurotoxicity when administered in vivo. ConBr (10 μg/site) administered via intracerebroventricular (i.c.v.) showed a neuroprotective activity against seizures induced by QA (36.8 nmol/site; i.c.v.) when administered 15 min prior to QA, with a percentage of protection around 50%. ConBr was also able to significantly decrease the severity of the seizures but without changes in the latency of the first convulsion or the duration of the seizures. This effect was dependent on the structural integrity of the ConBr protein and its binding capacity to oligosaccharides residues. ConA, a lectin with high similarity to ConBr, did not reverse the QA-induced seizures. Moreover, ConBr was able to protect against hippocampal cell death caused by QA, which was measured by propidium iodide incorporation. QA caused activation of JNK2 and improved the phosphorylation of Ser831 and 845 on the AMPA receptor GluR1 subunit, and both of these effects were counteracted by ConBr. Our data suggest that the lectin ConBr may exert a modulatory action on NMDA receptors, which inhibits its activity in response to QA.

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In vivo lymphocyte activation and apoptosis by lectins of the Diocleinae subtribe

Cited by 50 publications

References 19 publications

Influence of fetal bovine serum on cytotoxic and genotoxic effects of lectins in MCF‐7 cells

Influence of fetal bovine serum on cytotoxic and genotoxic effects of lectins in MCF‐7 cells

Embryotoxic activity and differential binding of plant-derived carbohydrate-recognizing proteins towards the sea urchin embryo cells

ConBr, a Lectin from Canavalia brasiliensis Seeds, Protects Against Quinolinic Acid-Induced Seizures in Mice

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