Establishment and characterization of a new continuous cell line from Lutzomyia longipalpis (Diptera: Psychodidae) and its susceptibility to infections with arboviruses and Leishmania chagasi

Rey, Gloria; Ferro, C.; Bello, Felio J.

doi:10.1590/s0074-02762000000100017

Cited by 29 publications

(39 citation statements)

References 38 publications

(23 reference statements)

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“…Cell growth in the primary cell cultures until the formation of the confluent monolayer was relatively slow, which is in agreement with previous studies (Rey et al 2000, Bello et al 2001, Sudeep et al 2009). The pattern of cell division and growth depended initially on the embryonic tissue fragments that adhered firmly to the flask surface 12 h after explantation and, at later times, the cells began to migrate and proliferate from these tissues.…”

Section: Discussionsupporting

confidence: 92%

“…The occurrence of vesicles in the cell cultures of insects and other arthropods is very common and has been demonstrated in several studies (Charpentier et al 1995, Rey et al 2000, Silva et al 2008). …”

Section: Discussionmentioning

confidence: 85%

“…quinquefasciatus larvae and pupae extracts from the same colony. The four isozymatic patterns from the cell cultures were compared with a cell line from Lutzomyia longipalpis (Lulo) (Rey et al 2000) and two isozymatic patterns were compared with a cell line from Lutzomyia spinicrassa (Zapata et al 2005). The relative electrophoretic mobility (REM) was calculated using the formula REM = e/a × 100, where "e" corresponds to the distance run in mm for each enzyme in the Cx.…”

Section: Methodsmentioning

confidence: 99%

“…In Colombia, Rey et al (2000), Bello et al (2001) and Zapata et al (2005) generated primary cell cultures and cell lines from different species of mosquitoes and sand flies that were capable of continuous growth. These cells were characterised morphologically, cytogenetically, biochemically and/or molecularly and, in some cases, were assessed for susceptibility to infection with arboviruses and parasites.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)

Segura

Santamaría

Cabrera

et al. 2012

Mem. Inst. Oswaldo Cruz

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 85%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)

Segura

Santamaría

Cabrera

et al. 2012

Mem. Inst. Oswaldo Cruz

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“…La presencia de dos grupos diferentes de células en los cultivos celulares derivados del mosquito A. aegypti se debe, posiblemente, a los diversos tipos de tejidos disponibles, a partir de huevos embrionados utilizados durante la formación de los cultivos primarios (Charpentier et al 1995, Rey et al 2000. Sin embargo, predominaron las formas de apariencia epitelioides, como lo registró Ardila et al (2005), diferente a lo observado en las líneas celulares Mos.…”

Section: Discussionunclassified

Morfología y citoquímica de cultivos celulares de Aedes aegypti (Diptera: Culicidae) y susceptibilidad a Leishmania panamensis (Kinetoplastida: Trypanosomatidae)

Miranda¹,

Sarmiento²,

Caldas³

et al. 2007

RBT

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Morphology and cytochemistry of Aedes aegypti's cell cultures (Diptera: Culicidae) and susceptibility to Leishmania panamensis (Kinetoplastida: Trypanosomatidae). The first cellular line of Aedes aegypti was developed by Grace in 1966; afterwards, other cellular lines of this species have been generated. These have been used for the study of pathogenic organisms like viruses, bacteria and parasites, which demonstrates their importance in biomedical applications. This research describes, for the first time, some cytochemical characteristics of A. aegypti cell cultures, that were infected with (MHOM/CO/87CL412) strain of Leishmania panamensis. A morphological study of the cell culture was also carried out. Maintenance of the cell culture, parasites and infection in vitro were carried out in the Laboratory of Entomology, Cell Biology and Genetics of the Universidad de La Salle. The cell cultures infected with the parasite were maintained in a mixture of mediums Grace/L15, supplemented with 10 % fetal bovine serum (FBS) at pH 6.8 and a temperature of 26 ºC, during 3, 6 and 9 post-infection days. After this, these cell cultures were processed through High Resolution Light Microscopy (HRLM) and Transmission Electron Microscopy (TEM) based on standard protocols defined by the Group of Microscopy and Image Analyses of the Instituto Nacional de Salud. Semi-fine slices of 1 µm colored with toluidine blue were used for the morphological analysis of the culture, and ultra fine cuts of 60 to 90 nm stained with uranyl acetate and lead citrate where used for the ultrastructural study. In addition, PAS and peroxidase staining was carried out in cells fixed with methanol. The morphometric study was analyzed with software ImageJ (NIH). In the semi-fine slices, small cells were observed showing fibroblastic appearance 10.84±2.54 µm in length and 5.31±1.26 µm wide; other cells had epithelial appearance with a great peripheral nucleus, voluminous and vacuolated cytoplasm, 23.04±4,00 µm in length and 13.96±3.70 µm wide. These last ones predominated over the ones with fibroblastic appearance. Regarding the PAS coloration, 7.08 % of the cells presented abundant PAS positive cytoplasmatic granules which indicated polysaccharides presence. The peroxidase test gave a negative result. The greatest percentage of infection (18.90 %) of one total of 101 cells, turned up by day 6. Some cells analyzed by TEM presented a vacuolated aspect cytoplasm; some contained parasites, other fibrillar material and others were empty. The results indicate that A. aegypti cell culture can support the internalization and transformation of the parasite, which demonstrates the capacity that these cell cultures have to be infected with L. panamensis and to maintain the infection for approximately one week. Rev. Biol. Trop. 56 (2): 447-458. Epub 2008 June 30.

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Establishment and characterization of an embryonic cell line from Gampsocleis gratiosa (Orthoptera: Tettigoniidae)

Zhang

Feng

Ding

et al. 2011

In Vitro Cell.Dev.Biol.-Animal

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The first continuous cell line from the embryo of Gampsocleis gratiosa (Orthoptera: Tettigoniidae), designated as RIRI-GG1, was established. This cell line was serially subcultured in modified Grace medium. The cells were grown adherent to a culture flask and had spindle-like and polygonal shapes. The chromosome number ranged from 26 to 79 at the 50th passage, and 68% of cells had a diploid chromosome number. The growth rate was determined at the 53rd passage, and the population doubling time was calculated to be 122.1 h. The rDNA internal transcribed spacer and the mitochondrial cytochrome c oxidase subunit I gene sequence analysis indicated that the RIRI-GG1 cell line was derived from G. gratiosa. This cell line had no apparent susceptibility to Autographa californica nucleopolyhedrovirus and Bombyx mori nucleopolyhedrovirus.

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Establishment and characterization of a new continuous cell line from Lutzomyia longipalpis (Diptera: Psychodidae) and its susceptibility to infections with arboviruses and Leishmania chagasi

Abstract: Embryonic tissue explants of the sand fly Lutzomyia longipalpis (Lutz & Neiva 1912)

Cited by 29 publications

References 38 publications

Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)

Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)

Morfología y citoquímica de cultivos celulares de Aedes aegypti (Diptera: Culicidae) y susceptibilidad a Leishmania panamensis (Kinetoplastida: Trypanosomatidae)

Establishment and characterization of an embryonic cell line from Gampsocleis gratiosa (Orthoptera: Tettigoniidae)

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