Genetic polymorphism among six Trypanosoma cruzi stocks isolated from chronic chagasic patients

Castro, Ana Maria de; Araújo, Thais Cristine de Carvalho; Jesuino, Rosália Santos Amorim; Soares, Célia Maria de Almeida; Ostermayer, Alejandro Luquetti

doi:10.1590/s0074-02761999000500017

Cited by 2 publications

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“…These findings corroborate the homology found in T. cruzi isolates obtained from chronic patients and does not associate with their respective clinical forms. However, DNA fragment patterns generated by RAPD allowed the distinction of T. cruzi stocks from chagasic patients but were not correlated with the different clinical forms (Castro et al 1999). Although a reduced number of T. cruzi strains were analyzed, an interesting finding is the equal association among strains by their biological characteristics and their RAPD patterns.…”

Section: Discussionmentioning

confidence: 99%

Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms

et al. 2006

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The genetic variability of 61 Trypanosoma cruzi isolates from 47 chronic chagasic patients of Minas Gerais state was analyzed by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) using M13-40, lambdagt11-F, and L15996 primers. Cluster analysis by unweighted pair group method analysis was applied to RAPD profiles, and cluster analysis used to verify a possible correlation among different clinical forms of the disease from these patients. The T. cruzi isolates showed distinct grouping on tree topology, with the isolates not being possible to establish a correlation to the clinical forms of Chagas' disease. These data showed that the T. cruzi isolates from these patients would compose a group of populations well correlated genetically.

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Section: Discussionmentioning

confidence: 99%

Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms

et al. 2006

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“…Internal transcribed spacer-restriction fragment length polymorphism (ITS-RFLP) and random amplification of polymorphic DNA (RAPD) techniques have been applied to evaluate T. cruzi variability (Steindel et al 1993;Bastrenta and Brenière 1998;Gomes et al 1998;de Castro et al 1999;Fernandes et al 1999;Santos et al 2002;Cuervo et al 2002;Rodríguez et al 2002). However, in Colombia, these methods have been employed by only a small number of groups of researchers Rodríguez et al 2002); indeed, most of the studies have focused on analyzing isoenzyme profiles and showing great heterogeneity (Widmer et al 1985;Saravia et al 1987;Rodríguez et al 1998;Triana et al 1999;Jaramillo et al 1999;Ruíz-García et al 2001;Montilla et al 2002).…”

Section: Introductionmentioning

confidence: 99%

ITS–RFLP- and RAPD-based genetic variability of Trypanosoma cruzi I, human and vector strains in Santander (Colombia)

et al. 2009

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Chagas disease is a severe public health problem in Latin-American countries. In Colombia, the predominance of Trypanosoma cruzi I has been described in the literature, with a broad heterogeneity between strains. However, most of the studies carried out centered on isoenzyme analysis, with a smaller number that focus on other molecular methods. In this report, we discuss the results of a molecular analysis of T. cruzi I strains, isolated from the domestic cycle, from the department of Santander, one of the territorial divisions where the prevalence of infection is highest. Internal transcribed spacer-restriction fragment length polymorphism and random amplification of polymorphic DNA were used to characterize 16 strains from human and vector (Triatominae) hosts. The data reveal a clustering based on the biological origin. Human and vector strains grouped separately; however, three vector strains clustered together with human strains. These results indicate that genetic differences exist in the strains that infect both hosts. We conclude that T. cruzi I populations in the domestic cycle of transmission of Chagas disease in Santander are heterogeneous and are composed of different clones. The epidemiological and biological implications are discussed.

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Genetic polymorphism among six Trypanosoma cruzi stocks isolated from chronic chagasic patients

Cited by 2 publications

References 0 publications

Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms

Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms

ITS–RFLP- and RAPD-based genetic variability of Trypanosoma cruzi I, human and vector strains in Santander (Colombia)

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