Eokines: synthesis, storage and release from human eosinophils

Lacy, P; Moqbel, Redwan

doi:10.1590/s0074-02761997000800017

Cited by 34 publications

(25 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[32][33][34] In addition to its granule content of cationic proteins such as ECP, major basic protein, and eosinophil peroxidase with known toxic effects on various cells and tissues including bladder epithelium, 35,36 eosinophils are now known to have the capacity to express, store, and release an array of up to 18 different cytokines, chemokines, and growth factors, including interleukin-3 (IL-3), IL-4, IL-5, IL-6, regulated upon activation normal T lymphocyte expressed and secreted (RAN-TES), granulocyte-macrophage-colony stimulating factor, tumor necrosis factor-␣, and transforming growth factor-␤. 37 Moreover, eosinophils have been shown to express major histocompatibility class II and CD4 molecules and act as antigen presenting cells in vitro. [38][39][40] These findings imply that eosinophils have both toxic and immune regulatory functions and suggest that eosinophils may play an important role in the regulation of the perioval inflammatory reactions.…”

Section: Discussionmentioning

confidence: 99%

Quantitative assessment of eosinophiluria in Schistosoma haematobium infections: a new marker of infection and bladder morbidity.

Reimert¹,

Mshinda²,

Hatz³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Eosinophiluria, as quantified by measuring eosinophil cationic protein (ECP) in urinary extracts, microhematuria, egg excretion, and ultrasound-detectable bladder pathology were recorded in Schistosoma haematobium-infected Tanzanian school children at a baseline survey and during an 18-month post-treatment followup study. Significant correlations were seen between urinary ECP levels, intensity of infection, and bladder pathology. Treatment resulted in a marked reduction in prevalence and intensity of infection, in a delayed and less marked reduction in ECP levels, and in a resolution of pathology. The overall diagnostic efficiency of the ECP test (cut-off value for the ECP Ն5 ng/ml) in relation to infection was comparable with that of egg count and microhematuria, but with a better sensitivity than a single egg count. In relation to bladder pathology, the diagnostic performance of the ECP test (cut-off value for the ECP Ն25 ng/ml) exceeded that of a single egg count. In addition, the ECP was better in discriminating between different grades of bladder pathology. The present study points to the ECP as a useful marker of both S. haematobium infection and of associated bladder morbidity reflecting the inflammatory status of the bladder wall.

show abstract

Section: Discussionmentioning

confidence: 99%

Quantitative assessment of eosinophiluria in Schistosoma haematobium infections: a new marker of infection and bladder morbidity.

Reimert¹,

Mshinda²,

Hatz³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…The latter secretory granules are the site of storage of cytotoxic cationic proteins as well as a number of cytokines, chemokines, and growth factors. 6,7 The membrane-bound crystalloid granule comprises 2 compartments: an electron-dense crystalline core (internum) where major basic protein (MBP) 8,9 is stored and an electron-lucent matrix 6 where 3 cationic proteins-namely, eosinophil cationic protein, eosinophil peroxidase (EPO), and eosinophil-derived neurotoxin 1,8 -together with a number of other cytokines, including interleukin-6 (IL-6) 10 and RANTES (regulated on activation normal T cells expressed and secreted), 11 are stored. Among eosinophilderived mediators, ␤-hexosaminidase (␤-hex) has been shown to localize to both small secretory vesicles and crystalloid granules.…”

Section: Introductionmentioning

confidence: 99%

Translocation of the tetraspanin CD63 in association with human eosinophil mediator release

Mahmudi‐Azer

Downey

Moqbel

2002

Blood

View full text Add to dashboard Cite

The tetraspanin CD63 (also known as LAMP-3) has been implicated in phagocytic and intracellular lysosome-phagosome fusion events. It is also present in eosinophils, with predominant expression on crystalloid granule membrane. However, its role in eosinophil function is obscure. We hypothesized that CD63 is associated with intracellular events involved in eosinophil activation and mediator release. We used a combination of confocal immunofluorescence microscopy, flow cytometry, and secretion assays, including ␤-hexosaminidase, eosinophil peroxidase, and RANTES, to examine CD63 expression, intracellular localization, and its association with cell activation and mediator release. In resting eosinophils, CD63 immunoreactivity was localized to plasma and crystalloid granule membranes. In interferon-␥ (IFN-␥)-or C5a/CB-stimulated cells (10 minutes), intracellular CD63 appeared to shift to the cell periphery and plasma membrane, while stimulation with a cocktail of interleukin-3 (IL-3)/IL-5/granulocyte-macrophage colony-stimulating factor induced the appearance of discrete intracellular clusters of CD63 immunoreactivity. IFN-␥ induced mobilization of CD63 to the cell periphery, which coincided with selective mobilization of RANTES prior to its release, implying CD63 association with piecemeal degranulation. Agonist-induced CD63 mobilization and cell surface upregulation was associated with ␤-hexosaminidase, eosinophil peroxidase, and RANTES release. Dexamethasone as well as genistein (a broad-spectrum inhibitor of tyrosine kinases) inhibited agonistinduced intracellular mobilization of CD63 and RANTES together with cell surface up-regulation of CD63 and mediator release. This is the first report of an association between CD63 mobilization and agonist-induced selective mediator release, which may imply the involvement of CD63 in eosinophil activation and piecemeal degranulation. IntroductionEosinophils are major effector cells in allergic inflammation and asthma. 1-3 They synthesize, store, and release a wide range of proinflammatory mediators, including at least 4 cationic proteins 1,2 and up to 23 cytokines and growth factors. 4,5 Eosinophils contain different populations of mediator-storage organelles, including small secretory vesicles as well as crystalloid granules. The latter secretory granules are the site of storage of cytotoxic cationic proteins as well as a number of cytokines, chemokines, and growth factors. 6,7 The membrane-bound crystalloid granule comprises 2 compartments: an electron-dense crystalline core (internum) where major basic protein (MBP) 8,9 is stored and an electron-lucent matrix 6 where 3 cationic proteins-namely, eosinophil cationic protein, eosinophil peroxidase (EPO), and eosinophil-derived neurotoxin 1,8 -together with a number of other cytokines, including interleukin-6 (IL-6) 10 and RANTES (regulated on activation normal T cells expressed and secreted), 11 are stored. Among eosinophilderived mediators, ␤-hexosaminidase (␤-hex) has been shown to localize to both small secretory vesicle...

show abstract

“…It is known that both isolated or in synergism with IL-5, eotaxin is capable of releasing eosinophils from the bone marrow to the blood stream. 22 Lacy and Moqbel (1997) 23 concluded that the importance of the synthesis of GM-CSF for eosinophils resides in autocrine and paracrine actions, capable of activating and prolonging the survival of their own in vivo eosinophils.…”

Section: Discussionmentioning

confidence: 99%

Efeito da mitomicina C na secreção de fator estimulador de granulócitos macrófagos e interleucina-5 em cultura de estroma de pólipos nasais eosinofílicos

Crosara¹,

Vasconcelos

Guimarães

et al. 2005

Rev. Bras. Otorrinolaringol.

View full text Add to dashboard Cite

The research involving tissue factors, such as granulocyte macrophage colonies stimulating factor (GM-CSF) and interleukin 5 (IL-5), leads to the mechanisms involved in the maintenance of eosinophilia, which is essential for the pathogenesis on eosinophilic nasal polyps. Mitomycin C has been successfully used in otolaryngology. Aim: The objective of this study was to evaluate the effect of mitomycin C in secretion of GM-CSF and IL-5 on eosinophilic nasal polyps. Study design: case-control. Material and Method: This is a comparative and auto-matched experimental study, performed with fragments of polyps which had been obtained from biopsy of patients with eosinophilic nasosinusal polyposis. The fragments of the experimental group were treated with mitomycin C (400 microg/ml) for 5 minutes and then washed in RPMI substrate. At time zero, 12 and 24 hours, the surface material was taken to determination of its GM-CSF levels in 22 patients and of IL-5 levels in 19 patients, by ELISA method. Results: Reduction in GM-CSF expression on the experimental group at time 24 h (p<= 0.05). The treated group presented significant GM-CSF expression between zero time and 12 h time (p=0.013) showing the culture viability such as in the non-treated group. Tendency to decreasing IL-5 levels on the treated groups at 24 hours. Conclusion: This study showed that mitomycin C was efficient in inhibiting GM-CSF synthesis with reduction of IL-5 secretion, but this fact needs complementary studies.

show abstract

Eokines: synthesis, storage and release from human eosinophils

Cited by 34 publications

References 47 publications

Quantitative assessment of eosinophiluria in Schistosoma haematobium infections: a new marker of infection and bladder morbidity.

Quantitative assessment of eosinophiluria in Schistosoma haematobium infections: a new marker of infection and bladder morbidity.

Translocation of the tetraspanin CD63 in association with human eosinophil mediator release

Efeito da mitomicina C na secreção de fator estimulador de granulócitos macrófagos e interleucina-5 em cultura de estroma de pólipos nasais eosinofílicos

Contact Info

Product

Resources

About