Isolation of fungi from nature in the region of Botucatu, state of São Paulo, Brazil, an endemic area of paracoccidioidomycosis

Pimenta

Rev. Soc. Bras. Med. Trop.

et al. 2012

Introduction:Little is known about the early events in the interaction between Paracoccidioides brasiliensis and its host. To understand the effect of carbohydrates in the interaction between the fungus and epithelial cell in culture, we analyzed the influence of different carbohydrate solutions on the adhesion of P. brasiliensis yeast cells to CCL-6 cells in culture. Methods: Fungal cells were cultivated with the epithelial cell line, and different concentrations of D-fucose, N-acetyl-glucosamine, D-mannose, D-glucosamine, D-galactosamine, sorbitol and fructose were added at the beginning of the experiment. Six hours after the treatment, the cells were fixed and observed by light microscopy. The number of P. brasiliensis cells that were adhered to the CCL-6 monolayer was estimated. Results: The number of adhesion events was diminished following treatments with D-fucose, N-acetyl-glucosamine, D-mannose, D-glucosamine and D-galactosamine as compared to the untreated controls. Sorbitol and fructose-treated cells had the same adhesion behavior as the observed in the control. P. brasiliensis propagules were treated with fluorescent lectins. The FITC-labeled lectins WGA and Con-A bound to P. brasiliensis yeast cells, while SBA and PNA did not. Conclusions: The perceptual of adhesion between P. brasiliensis and CCL-6 cells decreased with the use of D-mannose, N-acetyl-glucosamine and D-glucosamine. The assay using FITC-labeled lectins suggests the presence of N-acetyl-glucosamine, α-mannose and α-glucose on the P. brasiliensis cell surface. An enhanced knowledge of the mediators of adhesion on P. brasiliensis could be useful in the future for the development of more efficient and less harmful methods for disease treatment and control.

Section: Paracoccidioidomycosis (Pcm) Is a Disease Endemic In Latinmentioning

confidence: 99%

The influence of carbohydrates in the interaction of Paracoccidioides brasiliensis with CCL-6 cells in vitro

Pimenta

Rev. Soc. Bras. Med. Trop.

et al. 2012

“…A espécie em questão difere de V. tectonae Kamal & Kumar pelos conídios clavados; de V. verrucosa Geeson, por apresentar conídios marcadamente elipsóides e verrucosos; de V. carlinae Ellis, por apresentar células conidiogênicas cicatrizadas e de V. constricta Mustafa & Abdul-Wahid pela profunda constrição no septo (Moustafa & AbdulWahid 1990). Montenegro et al (1996) isolaram V. botryosa pela primeira vez para o Brasil em amostras de solo do município de Botucatu, Estado de São Paulo.…”

Section: Pleurotheciopsisunclassified

“…Distribuição geográfica: Brasil, China (Montenegro et al 1996), Cuba (Mercado-Sierra & Castañeda-Ruíz 1987), Escócia (Farr et al 2007), Equador, Peru (Matsushima 1993), Ilhas Salomão (Matsushima 1971), Índia (Rao & Hoog 1986), Itália (Ellis 1971), Reino Unido (Kirk & Spooner 1984).…”

Section: Veronaea Botryosaunclassified

Fungos anamórficos (Hyphomycetes) da Chapada Diamantina: novos registros para o Estado da Bahia e Brasil

2007

“…Descrição taxonômica e ilustração não foram encontradas para o táxon em questão, motivo pelo qual incluem-se aqui. Montenegro et al (1996), em trabalho sobre isolamento de fungos patogênicos de solo da região de Botucatu, Estado de São Paulo, isolaram a espécie e informaram ser aquele o primeiro relato para a América do Sul, sem fornecer dados morfológicos. O material foi obtido em apenas uma amostra e os conidióforos apresentaram-se menores que em outros isolamentos (Ellis 1971, Mercado Sierra & Castañeda-Ruíz 1987.…”

Section: Rap Grandi and Tv Silva Sn (Sp307969)unclassified

Fungos anamorfos decompositores do folhedo de Caesalpinia echinata Lam.

Grandi

2006

Rev. bras. Bot.