Glycobiology of laminin-integrin interaction and the metastatic phenotype

Glycosylation of Integrins in Melanoma Progression

“…The presence of β1,6-branched complex-type oligosaccharides on the integrin receptors that bind LN and FN was first shown by Chammas et al in mouse melanoma cell line B16-F10 [70] and then confirmed on the β1 subunit sharing integrins in this parent cell line and its highly invasive B16-BL6 variant [73,84,85]. Significantly enhanced β1,6 branching found on highly invasive B16-BL6 cells resulted in their more efficient invasion and migration, as well as impaired adhesion to different ECM proteins (LN, FN, VN, type I and type IV collagen, hyaluronic acid, and Matrigel).…”

“…The vast majority of studies on integrin glycosylation in melanoma have used mouse melanoma cell line B16-F10 and phenotypic variants of it that show different degrees of invasive potential, mainly the weakly invasive cell lines B16-F1 or B16-Flr, and B16-BL6 cells selected for their higher ability to metastasize to the lungs [60,67,[69][70][71][72][73], as well as human melanoma cell lines derived from each stage of melanoma progression, most of which were established by Herlyn's group [3].…”

Matriz extracelular e enzimas degradatórias na hematopoese e doenças onco-hematológicas

“…As interações entre as células e a MEC são cruciais para determinar os padrões de comportamento celular, tais como crescimento, morte, diferenciação e motilidade que, por sua vez, apresentam importância em diversos mecanismos, como morfogênese, inflamação, resposta imune, invasão parasitária, transformação celular e metástase. 9 A interação de células tumorais com a membrana basal e a MEC no processo de invasão tumoral pode ser dividida em três etapas: 1 -Degradação da MEC por enzimas secretadas pelas células tumorais: metaloproteinases, colagenases, plasmina, catepsinas, glicosidases e heparanases. Estas enzimas estão associadas com a invasão, pois levam à desorganização e à fragmentação dos componentes do estroma e da membrana basal; 2 -Adesão da célula tumoral via receptores específicos da superfície celular, que geralmente interagem com componentes da MEC; 3 -Locomoção da célula tumoral na região da MEC previamente degradada pelas enzimas.…”

Integrins and Metastases: An Overview

“…We have been able to demonstrate that piintegrin synthesis or sorting is highly sensi tive to tunicamycin, thus providing a molec ular basis for the morphologic changes ob served after such treatment (especially rounding in shape) [102]. Moreover, studying the effect of swainsonine on B16-F10 cells, we and other groups have shown a decrease in lung-colonizing potential, paral lel to a subtle, but significant, decrease in adhesion to laminin and a delay in the spreading of swainsonine-treated cells on that substrate [103][104][105], Such alterations can be related to terminal galactosyl resi dues present in oligosaccharides displayed in a laminin-binding integrin from B16-F10 [R.C. R.R.B., in preparation].…”