Isolation of leishmania guyanensis from lesions of the nasal mucosa

Naiff, R. D.; Talhari, S.; Barrett, Toby Vincent

doi:10.1590/s0074-02761988000400022

Cited by 24 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cases of ML have been associated with multiple species [7], [8], [9], but our record of cases of ML caused by L. (V.) guyanensis is unusual. It was previously believed that the occurrence ML caused by L. (V.) guyanensis was extremely low, with only isolated cases having been described.…”

Section: Discussionmentioning

confidence: 66%

See 1 more Smart Citation

Mucosal Leishmaniasis Caused by Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in the Brazilian Amazon

et al. 2011

View full text Add to dashboard Cite

Background Leishmania (Viannia) braziliensis is a parasite recognized as the most important etiologic agent of mucosal leishmaniasis (ML) in the New World. In Amazonia, seven different species of Leishmania, etiologic agents of human Cutaneous Leishmaniasis, have been described. Isolated cases of ML have been described for several different species of Leishmania: L. (V.) panamensis, L. (V.) guyanensis and L. (L.) amazonensis.Methodology Leishmania species were characterized by polymerase chain reaction (PCR) of tissues taken from mucosal biopsies of Amazonian patients who were diagnosed with ML and treated at the Tropical Medicine Foundation of Amazonas (FMTAM) in Manaus, Amazonas state, Brazil. Samples were obtained retrospectively from the pathology laboratory and prospectively from patients attending the aforementioned tertiary care unit.ResultsThis study reports 46 cases of ML along with their geographical origin, 30 cases caused by L. (V.) braziliensis and 16 cases by L. (V.) guyanensis. This is the first record of ML cases in 16 different municipalities in the state of Amazonas and of simultaneous detection of both species in 4 municipalities of this state. It is also the first record of ML caused by L. (V.) guyanensis in the states of Pará, Acre, and Rondônia and cases of ML caused by L. (V.) braziliensis in the state of Rondônia.Conclusions/Significance L. (V.) braziliensis is the predominant species that causes ML in the Amazon region. However, contrary to previous studies, L. (V.) guyanensis is also a significant causative agent of ML within the region. The clinical and epidemiological expression of ML in the Manaus region is similar to the rest of the country, although the majority of ML cases are found south of the Amazon River.

show abstract

Section: Discussionmentioning

confidence: 66%

“…Because of this, ML is an important public health problem and neglected disease in the Brazilian Amazon [5], [6]. L. (V.) panamensis , L. (V.) guyanensis and L. (L.) amazonensis have also been associated with ML, but very few cases of ML have been associated with L. (V.) guyanensis [7], [8], [9].…”

Section: Introductionmentioning

confidence: 99%

Mucosal Leishmaniasis Caused by Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in the Brazilian Amazon

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Review of the literature shows a few reports on Leishmania species causing Old and New Word ML (Table 2). 6,8,16,[22][23][24][25][26][27][28][29][30][31][32][33][34]35 Mucosal L. tropica infection has been reported in Saudi Arabia, 6 whereas L. major and occasionally L. infantum have been isolated from the patients with ML in Sudan 31 and Europe, 36 respectively.…”

Section: Discussionmentioning

confidence: 99%

ThreeLeishmania/L.species –L. infantum,L. major,L. tropica– as causative agents of mucosal leishmaniasis in Iran

Shirian

Oryan

Hatam

et al. 2013

Pathogens and Global Health

View full text Add to dashboard Cite

Cases of human oro-mucosal leishmaniasis are mainly reported in areas where Leishmania (Viannia) braziliensis perpetuates and the damages are mainly located at the cartilaginous nasal septum and frontal portions of the nasal fossa. In Iran, an area free of any L.(V) braziliensis, three Leishmania species are known to perpetuate through distinct (i) blood-feeding sand flies and (ii) rodents or (iii) canidae. Thus while establishing the diagnosis of any human oro-mucosal lesions, three Leishmania species -L. infantum, L. major, and L. tropica -must be considered as potential etiological agents of these damages. With these objectives in mind, features such as localization, extent, severity of oro-mucosal lesions, and duration of symptoms at the time of diagnosis were recorded from 11 patients with respect to the presence or absence of cutaneous lesions in other body parts. The biopsy samples were collected from the oromucosal and cutaneous lesions and were processed for further identification of the Leishmania species. The lesions ranged from mucosal nodules without ulceration, nodules with erosion, and shallow to deep ulcerations. Leishmania major was isolated from six (55%) cases showing lesions or scars. The scars were restricted to upper and lower extremities. For the other five patients who did not display any signs of former or active cutaneous leishmaniasis, L. major, L. tropica, and L. infantum were isolated from their lesions. In conclusion L. major, L. infantum, and L. tropica, regardless of common tropism, can be seen in mucosal tissues. However, L. major was the predominant species detected from the lesions in the nasal, gingival, and hard and soft palates, and L. tropica was isolated from the gingival and lower lip lesions. Leishmania infantum was isolated from two severe cases of deep mucosal damage displayed by the epiglottis, cricoarytenoid muscle, and laryngeal mucosa. One important finding was the association of L. major with active or scarred skin lesions.

show abstract

“…Although the principal causative agent of MCL is L. (V.) braziliensis , other Leishmania species such as L. (V.) guyanensis, L. (V.) panamensis and L. (L.) amazonensis have been reported to affect mucosal tissues [6–8]. …”

Section: Leishmaniasismentioning

confidence: 99%

Molecular Epidemiology for Vector Research on Leishmaniasis

Kato

Gómez

Cáceres

et al. 2010

IJERPH

View full text Add to dashboard Cite

Leishmaniasis is a protozoan disease caused by the genus Leishmania transmitted by female phlebotomine sand flies. Surveillance of the prevalence of Leishmania and responsive vector species in endemic and surrounding areas is important for predicting the risk and expansion of the disease. Molecular biological methods are now widely applied to epidemiological studies of infectious diseases including leishmaniasis. These techniques are used to detect natural infections of sand fly vectors with Leishmania protozoa and are becoming powerful tools due to their sensitivity and specificity. Recently, genetic analyses have been performed on sand fly species and genotyping using PCR-RFLP has been applied to the sand fly taxonomy. In addition, a molecular mass screening method has been established that enables both sand fly species and natural leishmanial infections to be identified simultaneously in hundreds of sand flies with limited effort. This paper reviews recent advances in the study of sand flies, vectors of leishmaniasis, using molecular biological approaches.

show abstract

Isolation of leishmania guyanensis from lesions of the nasal mucosa

Cited by 24 publications

References 0 publications

Mucosal Leishmaniasis Caused by Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in the Brazilian Amazon

Mucosal Leishmaniasis Caused by Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in the Brazilian Amazon

ThreeLeishmania/L.species –L. infantum,L. major,L. tropica– as causative agents of mucosal leishmaniasis in Iran

Molecular Epidemiology for Vector Research on Leishmaniasis

Contact Info

Product

Resources

About