Natural lectin activity in the haemolymph of Panstrogylus megistus (Heteroptera: Reduvidae)

Gomes, Yara M.; Furtado, André Freire; Carvalho, Alexandre B. de

doi:10.1590/s0074-02761988000400018

Cited by 10 publications

(3 citation statements)

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“…This is in line with research Gomes (1988) that examined the natural activity of lectins on haemolymph Pangstrongylus megistus through hemagglutinasi test, it was found that in the supernatant and pellet P. megistus contained lectin activity in which the highest hemagglutination titer test was obtained from rabbit erythrocytes.…”

Section: Table 2 Agglutionation Titer Using Vertebrate Erythrocytessupporting

confidence: 87%

The effect of leaf biopesticide Mirabilis jalapa and fungi Metarhizium anisopliae to immune response and mortality of Spodoptera exigua instar IV

Suryani¹,

Anggraeni²

2014

AIP Conference Proceedings

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Spodoptera exigua is one of insect causing damage in agriculture sector. This insect can be controlled by a natural biopesticide by combining two agents of biological control, biopesticides Mirabilis jalapa and entomopathogenic fungi Metarhizium anisopliae, considered to be virulent toward a wide range of insects. The objective of research was to determine the effect of biopesticides M. jalapa and fungi M. anisopliae against immune system and mortality of S. exigua. This research used a complete randomized block design with five concentrations Mirabilis jalapa and optimum dose of M. anisopliae. A high dose of M. jalapa (0.8% w/v) is the most effective one to decrease total haemocytes especially granulocyt and plasmatocyt (cellular immune) and decrease the concentration of lectin (humoral immune) from S. exigua (p < 0.05). The combination of M. jalapa (0, 8% w/v) and lethal dose of M. anisopliae 2.59 x 10 7 spore/ml were significant to increase mortality of S. exigua within 48 hours (p < 0.05).

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Section: Table 2 Agglutionation Titer Using Vertebrate Erythrocytessupporting

confidence: 87%

The effect of leaf biopesticide Mirabilis jalapa and fungi Metarhizium anisopliae to immune response and mortality of Spodoptera exigua instar IV

Suryani¹,

Anggraeni²

2014

AIP Conference Proceedings

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“…Soluble lectins occur in a variety of species (Gold and Balding, 1975;Yeaton, 1982;Ey and Jenkin, 1982;Vasta and Marchalonis, 1983;Ratcliffe, 1986;Ratcliffe et aL, 1996;Richards and Ratcliffe, 1990;Osir, et al, 1995). Agglutination of parasites and erythrocytes by lectins has been reported in DipteraGlossinidae (Ingram and Molyneux, 1988;1993;Osir et al, 1995;Maudlin and Welburn, 1994); sandflies (Wallbanks et al, 1986); black flies (Ham and Smail, 1988); mosquitoes (Mohammed and Ingram, 1994;Mohamed et al, 1992); and Hemiptera -reduviid bugs (Pereira et al, 1981;Gomes et al, 1988;Barrocco and Loch, 1988;Mello et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Lectins in the brown ear tick,Rhipicephalus appendiculatus: detection and partial characterization

Sebitosi¹,

Kaaya²,

Young

et al. 1998

International Journal of Acarology

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Lectins (agglutinins) were detected and isolated from the hemolymph and gut of Rhipicephalus appendiculatus, the vector of Theileriaparva, using various vertebrate red blood cells (RBC).Although the hemolymph lectin agglutinated RBC's of horse at the titre of 4096, mouse (2048), rabbit (512) and rat (128), it did not agglutinate those of domestic animal tick hosts, e.g. cow, goat and sheep. Erythrocytes from some wildlife hosts showed agglutination, with rhino showing the highest titre (4096), zebra (512) and giraffe (256). Virtually no agglutination was observed with erythrocytes from Thomson's gazelle, and the oryx and buffalo gave no agglutination at all. The hemolymph lectin was inhibited by Xylose, N-acetyl galactosamine and partially by 13-d-Fructose. The gut lectin gave an agglutination titre of 4096, with rabbit erythrocytes, mouse (512), horse (4) and 16 for oryx, giraffe and Thomson's gazelle. It also did not agglutinate cattle or sheep erythrocytes but showed trace agglutination (2) for goat rbcs. The gut lectin was inhibited by D(-) mannosamine, D(+) glucosamine, D(+) galactosamine and Fetuin. Free lectins were not detected in the gut lumen, but in the gut integral membrane. The heterogeneity in the agglutination of the different erythrocytes suggests differences in the binding affinity and quantities of lectins or presence of several different lectins. If the lectin binding affinity of the erythrocyte surface membrane of T. parva infected cells is identical to that of uninfected erythrocytes then, the fact that the hemolymph and gut lectins did not agglutinate the domestic animal erythrocytes might be due to evolutionary adaptations and may influence disease transmission.

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“…Berdasarkan tabel diatas, hasil uji HA diperoleh titer agglutinasi tertinggi dari uji menggunakan eritrosit vertebrata, dikarenakan lektin invertebrata (serangga) teragglutinasi terhadap molekul asing secara in vitro dengan menggunakan eritrosit vertebrata (Gul dan Uji Hemaglutinasi Assay pada Hemolimf Spodoptera exigua yang diberi Perlakuan Botani e- ISSN 2654-5160 p-ISSN 1411 Avyali, 2001). Dalam penelitian ini digunakan eritrosit kelinci karena hal ini sejalan dengan penelitian Gomes et al, (1988) yang meneliti aktifitas natural lektin pada hemolimf Pangstrongylus megistus melalui uji hemagglutinasi, menemukan bahwa pada supernatan dan pelet P. megistus terdapat aktifitas lektin dimana, titer hemagglutinasi tertinggi diperoleh dari hasil uji eritrosit kelinci.…”

Section: Prosedur Penelitianunclassified

Uji Hemaglutinasi Assay pada Hemolimf Spodoptera exigua yang diberi Perlakuan Botani Pestisida

Suryani

Anggraeni

2019

bionature

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The larval stage of the armyworm (Spodoptera exigua), has a high and polyphagous feeding activity so that these insects have the potential to be pests in agriculture. The use of botanical pesticides in this case Mirabilis jalapa, is expected to control agricultural pests so that research on the immune system of S. exigua larvae is carried out by observing lectins that play a role in analyzing foreign molecules that enter the insect's body. The results were obtained, the higher the M. jalapa concentration, at the concentration of M. jalapa 0.4% and 0.8%, the lower the hemolymf agglutination titers. This is thought to decrease the binding capacity of lectins to foreign molecules. So that M. jalapa has the potential to be used as an environmentally friendly botanical pesticide.

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Natural lectin activity in the haemolymph of Panstrogylus megistus (Heteroptera: Reduvidae)

Cited by 10 publications

References 0 publications

The effect of leaf biopesticide Mirabilis jalapa and fungi Metarhizium anisopliae to immune response and mortality of Spodoptera exigua instar IV

The effect of leaf biopesticide Mirabilis jalapa and fungi Metarhizium anisopliae to immune response and mortality of Spodoptera exigua instar IV

Lectins in the brown ear tick,Rhipicephalus appendiculatus: detection and partial characterization

Uji Hemaglutinasi Assay pada Hemolimf Spodoptera exigua yang diberi Perlakuan Botani Pestisida

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