Effects of Laurus nobilis (Lauraceae) on Biomphalaria glabrata (Say, 1818)

Abstract

Experiments were carried out using aqueous extracts from leaves and flowers of Laurus nobilis on Biomphalaria glabrata. Treatments were performed on blastula stage (+/- 15 h after first cleavage) and on adult snails (11-18 mm). In both instances they were exposed for 24 h to different concentrations of the extracts on snails (200 to 2500 ppm) and embryos (20 to 300 ppm) at 25 +/- 1 degree C. The embryos were observed for a period of 20 days after treatment and the snails for 10 days. Results obtained with leaf aqueous extracts have shown a degree of toxicity on embryos starting at a concentration of 125 ppm, the flower extract being effective at 35 ppm. The malformation obtained with the different concentrations falls into the unespecific type category, however some cephalic and shell malformations were found in embryos treated with concentrations over 50 ppm (leaves) and 25 ppm (flowers). The LD90 on adult snails obtained by treatments with flower and leaf extract was observed at concentrations of 340 ppm and 1900 ppm respectively.

Molluscicidal activity of some marine substances against the snail Biomphalaria glabrata (Mollusca, Planorbidae)

“…The normal embryonic development has been described in detail (Camey and Verdonk 1970;Kawano et al 1992) and showed well-defined and easily recognizable stages allowing the detection of morphogenetic effects. It has been used at the Butantan Institute for more than 30 years to evaluate the mutagenicity and toxicity of chemical (Estevam et al 2006;Kawano et al 1979;Kawano and Simões 1986;Nakano et al 2003;Oliveira-Filho et al 2010;Rapado et al 2011;Ré and Kawano 1987) and physical agents (Okazaki et al 1996;Tallarico et al 2004). In this work, crude extracts of marine organisms from northern coastline of São Paulo State (Brazil) were assessed to detect molluscicidal and ovicidal properties against B. glabrata.…”

Establishment of the dominant lethal test in the freshwater mollusk Biomphalaria glabrata (Say, 1818)

“…The normal embryonic development has been described in detail [12,13] and showed well-defined and easily recognizable stages, allowing the detection of morfogenetic effects. This organism has been used in our laboratory to evaluate toxic and mutagenic effects of chemical and physical agents using mortality index, alterations in embryonic development and chromosome aberrations as biomarkers [14][15][16][17][18].…”