Analysis of antibody responses of Schistosoma mansoni infected patients against schistosomal antigens

Corrêa-Oliveira, Rodrigo; Oliveira, Guilherme; Golgher, Denise; Viana, Iramaya R. C.; Colley, Daniel G.; Carvalho, Omar S.; Rocha, Roberto Sena; Katz, Naftale; Gazzinelli, Giovanni

doi:10.1590/s0074-02761987000800031

Cited by 5 publications

(7 citation statements)

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“…Furthermore, since cross-protection with hookworm has been demonstrated [6] it is very important to evaluate the role of these antigens preventing Schistosoma mansoni infection.…”

Section: Kripao Bolivarmentioning

confidence: 99%

“…This reflects past infections since antibodies persist for long periods of time after cure [5]. False positive results could also be caused by cross-reactivity with other intestinal parasitic infections [6,7]. This latter point becomes a problem when ELISA with crude S. mansoni antigens are used for epidemiologic surveillance in endemic areas where intestinal parasites are often prevalent.…”

Section: Introductionmentioning

confidence: 99%

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Do intestinal parasites interfere with the seroepidemiologic surveillance ofSchistosoma mansoniinfection?

et al. 1996

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SUMMARYIn view of the known cross-reactivity of sera from patients with intestinal parasites to some Schistosoma mansoni antigens, field work was conducted in an area of Venezuela non-endemic for schistosomiasis using the routine immunoenzymatic assay (ELISA) with soluble egg antigen (SEA). False positive reactions represented 15-3 % of the total population as determined by SEA-ELISA. SEA-immunoblotting of the false positive sera indicated that protein fractions of 91 and 80 kDa appear to be responsible for cross-reactivity. Sera from hookworm infected individuals produced a higher frequency and intensity of cross-reaction than other sera. SEA-fractions of 105, 54, 46, 42, 32,25 and 15 kDa were the most specific.

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“…Furthermore, since cross-protection with hookworm has been demonstrated [6] it is very important to evaluate the role of these antigens preventing Schistosoma mansoni infection.…”

Section: Kripao Bolivarmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Do intestinal parasites interfere with the seroepidemiologic surveillance ofSchistosoma mansoniinfection?

et al. 1996

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“…The newborns demonstrated the same IgG anti-schistosome antibody activity as their mother. (Correa-Oliveira, Oliveira et al 1987;Novatosilva, Gazzinelli et al 1992) This differed from naive individuals from non-endemic areas, who received their first contact later in life. Furthermore, the production of anti-idiotypic T lymphocytes can begin in utero due to idiotypic sensitisation from maternal anti-schistosome antibodies that express the relevant idiotypes.…”

Section: Humoural Responsementioning

confidence: 96%

“…This technique can be used to diagnose the degree of morbidity and also judge 20 effectiveness of disease control programs over time. (Homeida, Ahmed et al 1988;de Jesus, Miranda et al 2000) Other methods, such as differences in antigenic recognition, have been postulated as diagnostic tools for disease form in individuals infected with schistosomiasis (Correa-Oliveira, Oliveira et al 1987) but suitable candidate proteins for such tools are still being sought.…”

Section: Chemotherapies and Diagnosticsmentioning

confidence: 99%

“…If, as we believe, distinct differences exist in the antigen specificity, disease form-specific antigen/antibody interactions could serve as indicators of chronic disease, and the uniquely recognized antigens could be developed for diagnostic tools that take advantage of differences in antigenic recognition in individuals infected with schistosomiasis. (Correa-Oliveira, Oliveira et al 1987) The experiments in this chapter aimed to determine whether the antibodies in MSS or HSS mouse sera were not only infection specific, but also if the antibody profiles were specific to either form. To this end, we tested whether differences in SEA specificity could be detected in mice with MSS or HSS and in pooled, purified anti-SEA antibody preparations.…”

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confidence: 99%

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Identifying Key Antigens that Drive Antibody-Mediated Regulation of Chronic Schistosomiasis

Jaurigue¹

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Chronic schistosomiasis is a spectral illness where patients present with either moderate intestinal (INT) schistosomiasis or severe hepatosplenic schistosomiasis (HS). The different clinical manifestations are associated with the immune response in the patient against schistosome eggs that end up lodged in the liver and other organs. Inbred male CBA/J mice infected with Schistosoma mansoni present with two distinct syndromes, moderate splenomegaly syndrome (MSS) and hypersplenomegaly syndrome (HSS); the pathologies of which parallel human INT and HS forms, respectively. Furthermore, antibody with a cross-reactive idiotype (CRI) is produced in MSS mice and INT patients (Montesano, Freeman et al. 1990a) that is absent in HSS mice and HS patients and is believed to play a protective role against severe pathology. Our primary aim was to determine the specific antigens in soluble egg antigen (SEA) that react to protective CRI+ anti-SEA antibodies using the CBA/J mouse model of disease. Using one-dimensional Western blotting, we have shown that the specificities of anti-SEA antibodies present in MSS and HSS forms of schistosomiasis are different. Using two-dimensional differential in-gel electrophoresis, we isolated six spots that were of significant interest as disease-specific candidates and a further six candidates that were infection-specific. Finally, we characterized these antigens using mass spectrometry, where we managed to identify several of these isolated antigens. Our infection-specific antigens can be useful in developing diagnostic tools to identify individuals infected withschistosomiasis, and individuals who are at risk of developing severe disease. Additionally, we found evidence for antigens that are specifically reactive to CRI+ antibodies and these can be used as candidates for the development of a vaccine that can drive the production of these protective antibodies. We also found evidence of CRI- antibody-specific antigens, and further analysis of these antigens may help increase our understanding of severe disease induction in chronic patients. Overall, this study identified several unique candidate antigens that merit further research in developing diagnostic and therapeutic tools, and added to our understanding of helminth interactions in human biology.

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Human schistosomiasis mansoni: Immune responses during acute and chronic phases of the infection

Caldas¹,

Campi-Azevedo²,

et al. 2008

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Analysis of antibody responses of Schistosoma mansoni infected patients against schistosomal antigens

Cited by 5 publications

References 0 publications

Do intestinal parasites interfere with the seroepidemiologic surveillance ofSchistosoma mansoniinfection?

Do intestinal parasites interfere with the seroepidemiologic surveillance ofSchistosoma mansoniinfection?

Identifying Key Antigens that Drive Antibody-Mediated Regulation of Chronic Schistosomiasis

Human schistosomiasis mansoni: Immune responses during acute and chronic phases of the infection

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