Inhibition of growth of Leishmania mexicana mexicana by Leishmania mexicana amazonensis during "in vitro" co-cultivation

Pacheco, Raquel S.; Júnior, Gabriel Grimaldi; Morel, Carlos Médicis

doi:10.1590/s0074-02761987000400011

Cited by 10 publications

(7 citation statements)

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“…We have shown evidences which suggest that the presence of L. guyanensis M4147 (noninfective) presumably inhibited the development of Endotrypanum, with the drastic reduction in the number of positive females, mainly in the group of females exposed to coinfection with the heterogeneous sample (M5725) of E. schaudinni. Effects of intra-or interspecific selective pressures have already been reported previously, among co-cultivated trypanosomatids (Pacheco et al 1987, Coppens et al 1992) and even about the biology of sand flies (Alekseev et al 1975, El-Sawaf et al 1994). On the other hand, the correlation between the massive infection found in the stomodeal valve of females coinfected with L. guyanensis IM4216 and the isoenzymatic char-acterization of isolates suggest that there had been selection in the culture medium.…”

Section: Discussionmentioning

confidence: 69%

Single and concomitant experimental infectionsby Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the Neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae)

Barbosa

Oliveira

Bertho

et al. 2006

Mem. Inst. Oswaldo Cruz

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Section: Discussionmentioning

confidence: 69%

Single and concomitant experimental infectionsby Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the Neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae)

Barbosa

Oliveira

Bertho

et al. 2006

Mem. Inst. Oswaldo Cruz

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“…It has been demonstrated that in co-cultivation of more than one Leishmania the dominant species tend to inhibit the growth of the other; consequently the degree of laboratory detection of such phenomenon remains unclear and likely underestimated [28, 42]. …”

Section: Discussionmentioning

confidence: 99%

Concomitant Infection withLeishmania donovaniandL. majorin Single Ulcers of Cutaneous Leishmaniasis Patients from Sudan

Babiker

Ravagnan

Fusaro

et al. 2014

Journal of Tropical Medicine

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In Sudan human leishmaniasis occurs in different clinical forms, that is, visceral (VL), cutaneous (CL), mucocutaneous (ML), and post-kala-azar dermal leishmaniasis (PKDL). Clinical samples from 69 Sudanese patients with different clinical manifestations were subjected to a PCR targeting the cytochrome oxidase II (COII) gene for Leishmania species identification. Mixed infections were suspected due to multiple overlapping peaks presented in some sequences of the COII amplicons. Cloning these amplicons and alignment of sequences from randomly selected clones confirmed the presence of two different Leishmania species, L. donovani and L. major, in three out of five CL patients. Findings were further confirmed by cloning the ITS gene. Regarding other samples no significant genetic variations were found in patients with VL (62 patients), PKDL (one patient), or ML (one patient). The sequences clustered in a single homogeneous group within L. donovani genetic group, with the exception of one sequence clustering with L. infantum genetic group. Findings of this study open discussion on the synergetic/antagonistic interaction between divergent Leishmania species both in mammalian and vector hosts, their clinical implications with respect to parasite fitness and response to treatment, and the route of transmission with respect to vector distribution and or adaptation.

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“…The different species and subspecies of Leishmania can be distinguished by monoclonal antibodies (24), isoenzyme analysis, or restriction digests of kDNA (26,29,34,35), but these techniques require a quantity of parasites obtainable only by in vitro culture.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of cutaneous leishmaniasis and species discrimination of parasites by PCR and hybridization

et al. 1994

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The aim of this study was to assess the efficacy of PCR methodology in establishing the diagnosis of cutaneous leishmaniasis in patients from areas of endemicity in Venezuela. Biopsies from 233 patients with cutaneous ulcers suggestive of leishmaniasis were analyzed by PCR, employing oligonucleotides directed against conserved regions of kinetoplast DNA (kDNA), and the PCR products were then hybridized to nonradioactively labeled, species-specific, cloned kDNA fragments. The ability of PCR to detect Leishmania cells was compared with those of the conventional methodologies: skin testing with killed promastigotes (Montenegro test), examination of Giemsa-stained biopsy smears, and in vitro culture of biopsy tissue. The PCR-hybridization technique detected the presence of Leishmania cells in 98% of patients clinically diagnosed as having leishmaniasis and also positive by the Montenegro skin test. In comparison, leishmania positivity was found in only 42% of cultures and 64% of biopsy smears. By hybridizing the PCR product to new kDNA * Corresponding author.

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Inhibition of growth of Leishmania mexicana mexicana by Leishmania mexicana amazonensis during "in vitro" co-cultivation

Cited by 10 publications

References 0 publications

Single and concomitant experimental infectionsby Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the Neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae)

Single and concomitant experimental infectionsby Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the Neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae)

Concomitant Infection withLeishmania donovaniandL. majorin Single Ulcers of Cutaneous Leishmaniasis Patients from Sudan

Diagnosis of cutaneous leishmaniasis and species discrimination of parasites by PCR and hybridization

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