Estabelecimento de colonia, em laboratório, de Lutzomyia intermedia Lutz & Neiva, 1912 (Diptera, Psychodidae, Phlebotominae)

Abstract

A laboratory colony of the sandfly Lutzomyia intermedia was established (at present in its seventh generation) in order to provide specimens for experimental infection and for experimental xenodiagnosis of natural infections due to cutaneous leishmanias in Rio de Janeiro. Date are presented on the methodology and on the yield and duration of each stage of development, during the first four generations.

Rearing and Colonization of Lutzomyia evansi (Diptera: Psychodidae), a Vector of Visceral Leishmaniasis in Colombia

“…longipalpis (KillickKendrick et al 1977); Lu. intermedia (Rangel et al 1985); Lu. whitmani (Barretto 1941), Ph.…”

Development of Lutzomyia intermedia and Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae) larvae in different diets

“…Such recipients were kept in the absence of light at 25ºC in the phlebotomine insectary of the Department of Entomology of the Oswaldo Cruz Institut, in Rio de Janeiro, State of Rio de Janeiro, Brazil, from where eggs of L. intermedia and L. longipalpis were obtained for the development of this research. Breeding method for these phlebotomine species was originally described by Rangel et al (1985).…”

Identification of the natural breeding sites of sandflies (Diptera: Psychodidae: Phlebotominae), potential vectors of leishmaniasis, in the province of Chaco, Argentina

“…The jars were then packed in Styrofoam boxes. Wet cotton was placed in the boxes to maintain a humid environment (Rangel et al 1985).…”

“…To identify adults, the key developed by Galati (2003) Rangel et al 1986, Andrade Filho et al 2004). The humidity was maintained with cotton soaked in distilled water and placed under the base of the jar (Rangel et al 1985). After the 60 days of observation, the soil was mixed with a supersaturated solution of sodium chloride as the flotation liquid for at least 20 min.…”