Detection of vanC 1 gene transcription in vancomycin-susceptible Enterococcus faecalis

Moura, Tiane Martin de; Cassenego, Ana Paula Vaz; Campos, Fabrício Souza; Ribeiro, Andréa Machado Leal; Franco, Ana Cláudia; d’Azevedo, Pedro Alves; Frazzon, Jeverson; Frazzon, Ana Paula Guedes

doi:10.1590/s0074-0276108042013009

Cited by 24 publications

(19 citation statements)

References 27 publications

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“…This reaffirms the importance of genetic resources as support for the equilibrium of family units [82]. However, this behavior is not uniform; other studies have described an exploitive relationship with the species in its distribution [83].…”

Section: Discussionsupporting

confidence: 65%

Use of the palm Euterpe edulismartius in landscape units managed by migrants of German origin in Southern Brazil

Milanesi

Peroni

Reis

2013

J Ethnobiology Ethnomedicine

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BackgroundPeople influence their environments through the manipulation of landscapes and species. Human influence on the landscape may lead to the development of differentiated landscape units that originate from past use and may be related to the presence of certain species. This study investigated the presence of the palm Euterpe edulis and its current and past importance in landscape units established by a community of German descendants located in southern Brazil. The objectives of this study were to characterize the use of the species, to identify the importance of E.edulis for the German immigrant community, to identify past and current uses of E.edulis, to describe the historical use of the landscape, and lastly, to identify landscape units in which E.edulis is found.MethodsThe researched community is composed of people of German descent residing in southern Brazil. A variety of research tools were used to achieve the objectives of the research. Semi-structured interviews and free-listings were conducted in all family units. The interviews focused on groups of people in the community who had current or historical connection with the species. Group workshops and guided tours were conducted to identify different landscape units. The data were analyzed using descriptive statistics, use-value index, citation frequency, salience index, and informant perception analysis.ResultsOver the historical period studied, the community demonstrated changes with respect to economic activities. These changes are reflected in the transformation of the landscape. The species E.edulis was and still is very important for people in the community; its importance is reflected in its high use value, citation frequency and salience. The species is found within various landscape units in the community as well as in homegardens and in secondary forests.ConclusionsThe landscape heterogeneity of this community is influenced by changes in economic activities and by the relationship with the conservation unit. Landscape units resulting from this relationship may be identified. The species E.edulis is found within these landscape units and is integrated into the livelihood of the community.

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Section: Discussionsupporting

confidence: 65%

Use of the palm Euterpe edulismartius in landscape units managed by migrants of German origin in Southern Brazil

Milanesi

Peroni

Reis

2013

J Ethnobiology Ethnomedicine

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“…This could explain absence of vanC 1 gene in our E. faecalis isolates, despite the existence of reported cases in the literature [48,49]. This gene may have been transferred from above enterococcal species to E. faecalis horizontally [48].…”

Section: Discussionmentioning

confidence: 72%

“…The vanC genes are intrinsic property (chromosomally encoded) of Enterococcus gallinarum, Enterococcus casseliflavus, and Enterococcus flavescens [12]. This could explain absence of vanC 1 gene in our E. faecalis isolates, despite the existence of reported cases in the literature [48,49]. This gene may have been transferred from above enterococcal species to E. faecalis horizontally [48].…”

Section: Discussionmentioning

confidence: 80%

High prevalence of vancomycin and high-level gentamicin resistance in Enterococcus faecalis isolates

Taji

Heidari

Ebrahim‐Saraie

et al. 2018

AMicr

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Multiple drug-resistant enterococci are major cause of healthcare-associated infections due to their antibiotic resistance traits. Among them, Enterococcus faecalis is an important opportunistic pathogen causing various hospital-acquired infections. A total of 53 E. faecalis isolates were obtained from various infections. They were identified by phenotypic and genotypic methods. Determination of antimicrobial resistance patterns was done according to CLSI guidelines. The isolates that were non-susceptible to at least one agent in ≥3 antimicrobial categories were defined as multidrug-resistant (MDR). Detection of antimicrobial resistance genes was performed using standard procedures. According to MDR definition, all of the isolates were MDR (100%). High-level gentamicin resistance was observed among 50.9% of them (MIC ≥ 500 μg/ml). The distributions of aac(6′)-Ie-aph(2′′)-Ia and aph(3′)-IIIa genes were 47.2% and 69.8%, respectively. The aph(2′′)-Ib, aph(2′′)-Ic, aph(2′′)-Id, and ant(4′)-Ia genes were not detected. Vancomycin resistance was found in 45.3% of strains. The vanA gene was detected in 37.7% of isolates, whereas vanB and vanC 1 genes were not observed in any strain. Erythromycin resistance rate was 79.2% and the frequencies of ermB and ermC genes were 88.6% and 69.8%, respectively. The ermA and msrA genes were not present in any of the isolates. Our data indicate a high rate of MDR E. faecalis strains. All of high-level gentamicin-resistant isolates carried at least one of aac(6′)-Ie-aph(2′′)-Ia or aph(3′)-IIIa genes. Distribution of vanA was notable among the isolates. In addition, ermB and ermC were accountable for resistance to erythromycin.

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“…Also selective pressure by drugs had a major role in spreading of resistance (2,4). Enterococci are also prominent for wide-range antibiotic resistant.…”

Section: Introductionmentioning

confidence: 99%

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Shahraki

Mousavi

2017

Jundishapur J Microbiol

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Background: The enterococci are responsible for infections including bacteremia and endocarditis which are usually resistant to multiple antibiotics. This nosocomial agent probably harbors putative virulence factors which increases their capability to colonize hospitalized patients. Objectives: This study was aimed in order to find the frequency of various virulence factors in enterococci and their relationship with multidrug resistance (MDR). Methods: The samples were collected from different hospital wards including; Intensive care unit (ICU), cardiac care unit (CCU), pediatrics department, internal wards, and transplantation. The isolates were detected by biochemical tests and in order to determine the antibiotic susceptibility pattern, disk diffusion agar (kirby-bauer) was accomplished. Then, MICs (Minimum inhibitory concentrations) of vancomycin were determined by E-test strips. For molecular examinations and detection of drug resistance genes, the simple polymerase chain reaction was used. The multiplex PCR was used in order to detect virulence factors. Results: Total of 85 isolates were obtained from one university teaching hospital in southeast of Iran. Approximately 95% of isolated which were from urine specimens and 34% of isolates were collected from pediatrics units at hospital. Tetracycline resistance (48%) has been observed with a high frequency and related to the tetM gene. Furthermore, eighteen isolates were recognized as MDR strains that carried vanA, aac (6)-Ie-aph (2)-Ia, ermB, and tetM genes. Among virulence factor genes, asa1 (69%) and gelE (55%) are more frequently observed in both species. In general, we found Enterococcus faecalis strains more prevalent. Also, E. faecium was related to antibiotic resistance genes in nosocomial infection. Conclusions:The data was indicated a high prevalence of multiple antibiotics resistance genes with virulence determinants in enterococci and also considered resistant isolate in pediatrics unit. The current results can be recommended in order to change new strategies for antibiotic therapy, because this serious pathogen is important for treatment and eradication in hospitals. Furthermore, the biofilm formation was regulated and constructed by virulence determinants that could be a candidate for enterococcal treatment.

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Detection of vanC 1 gene transcription in vancomycin-susceptible Enterococcus faecalis

Cited by 24 publications

References 27 publications

Use of the palm Euterpe edulismartius in landscape units managed by migrants of German origin in Southern Brazil

Use of the palm Euterpe edulismartius in landscape units managed by migrants of German origin in Southern Brazil

High prevalence of vancomycin and high-level gentamicin resistance in Enterococcus faecalis isolates

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

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